Selection of Beauveria bassiana sensu lato and Metarhizium anisopliae sensu lato isolates as microbial control agents against the boll weevil (Anthonomus grandis) in Argentina

Nussenbaum, Ana Laura; Lecuona, Roberto E.

doi:10.1016/j.jip.2012.01.010

Cited by 37 publications

(17 citation statements)

References 39 publications

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“…In contrast to the effects of fungal conidia (Lopes et al 2011; Sabry et al 2011; Khalid et al 2012; Nussenbaum et al 2012), researchers have paid more attention to the role of fungal secondary metabolites on host insects in recent years (Sokolova et al 1999; Xia et al 2000; Xia et al 2001; Zibaee et al 2009a; Zibaee et al 2011). Abdul-Wahid et al (2012) reported the pathogenicity of both a spore suspension and metabolite solution of two fungi, Trichoderma harzianum and Fusarium solani , against cockroaches ( Periplaneta americana ).…”

Section: Discussionmentioning

confidence: 99%

The Effect ofBeauveria brongniartiiand its Secondary Metabolites on the Detoxification Enzymes of the Pine Caterpillar,Dendrolimus tabulaeformis

Fan

Xie

Xue

et al. 2013

Journal of Insect Science

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The mortality of pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu (Lepidoptera: Lasiocampidae), larvae treated with Beauveria brongniartii (Saccardo) Petch (Hypocreales: Clavicipitaceae) conidia and cell-free culture supernatants enriched for the secondary metabolites of the fungus was investigated. In addition, the effects of the treatments on the activities of two insect-related defense response proteins, glutathione S-transferase (GST) and esterase (EST), were measured over time. Bioassays were performed using a range of fungal spore (6 × 105 through 6 × 107 spores/mL) and supernatant extract concentrations (5.5–550 µg/mL). The results showed that the mortalities of D. tabulaeformis larvae were closely related to the concentration of the conidia and the metabolites of B. brongniartii. The differences among the treatments all reached a significant level. The activities of the two detoxifying enzymes, GST and EST, in the larvae increased simultaneously post-treatment. After infection with the conidial suspensions, the highest GST activity appeared at 3 days, and the activities of the caterpillars infected with 6 × 106 spores/mL and 6 × 107 spores/mL were significantly higher than in the control. Using α-naphthyl, the highest activity of EST also appeared at 3 days, and the differences for the three different concentrations were significant. A similar trend of change in the EST activity was observed using β-naphthyl. After treatment with the secondary metabolite solution, the highest GST activity appeared at 6 hr, and significant differences were found both for the different durations (2, 4, 6, 12, 24, and 48 hr) and in the three concentration groups. When using α-naphthyl, the EST activity peak appeared at 24 hr, and the differences were significant among the durations of 2, 4, 6, 12, 24, and 48 hr. The effect of the concentration of the secondary metabolite solution notably induced the EST activity in the insects, and a similar result was obtained using β-naphthyl. The data suggest that B. brongniartii produces secondary metabolites that disable the immune mechanisms of D. tabulaeformis, allowing the fungus to overcome and then kill its host. It was concluded that both the conidial suspensions and the metabolites of B. brongniartii were toxic to D. tabulaeformis larvae.

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Section: Discussionmentioning

confidence: 99%

The Effect ofBeauveria brongniartiiand its Secondary Metabolites on the Detoxification Enzymes of the Pine Caterpillar,Dendrolimus tabulaeformis

Fan

Xie

Xue

et al. 2013

Journal of Insect Science

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show abstract

“…In contrast to the effects of fungal conidia (Lopes et al 2011;Sabry et al 2011;Khalid et al 2012;Nussenbaum et al 2012), researchers have paid more attention to the role of fungal secondary metabolites on host insects in recent years (Sokolova et al 1999;Xia et al 2000;Xia et al 2001;Zibaee et al 2009a;Zibaee et al 2011). Abdul-Wahid et al (2012) reported the pathogenicity of both a spore suspension and metabolite solution of two fungi, Trichoderma harzianum and Fusarium solani, against cockroaches (Periplaneta americana).…”

Section: Discussionmentioning

confidence: 99%

Bioefficacy of Alpinia galanga (Zingiberaceae) Rhizome Extracts, (E)-p-Acetoxycinnamyl Alcohol, and (E)-p-Coumaryl Alcohol Ethyl Ether Against Bactrocera dorsalis (Diptera: Tephritidae) and the Impact on Detoxification Enzyme Activities

Sukhirun¹,

Pluempanupat²,

Bullangpoti³

et al. 2011

jnl. econ. entom.

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“…2011). Detoxification enzymes significantly eliminate exotic chemcials and maintain regular physiological processes (Nussenbaum & Lecuona 2012).…”

Section: Discussionmentioning

confidence: 99%

Biochemical analysis and pathogenicity of entomopathogenic fungi to Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Naeem

Freed

Akmal

2020

Entomological Research

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Asiatic citrus psylla, Diaphorina citri, Kuwayama (Hemiptera: Liviidae) is an economic pest of citrus groves and a vector of the bacterium, Candidatus Liberibacter spp., one of the causative agents of citrus greening. In order to estimate the infectivity of six different isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea, fungal bioassay was performed on the adults of D. citri. Adults of D. citri were treated individually with 1 × 10 5 , 1 × 10 6 , 1 × 10 7 , 1 × 10 8 , 2 × 10 8 spores/mL fungal concentrations by the immersion method. Subsequent to fungal bioassay, treated D. citri were used to determine the levels of esterase and glutathione S-transferases (GST) enzymes over a period of 3-7 days. The mortality results suggest that I. fumosorosea isolates (If-02) caused 82.2% mortality on the seventh day of treatment. However, B. bassiana isolate (Bb-08) with lowest LC 50 (1.4 × 10 7 spores/mL) proved to be highest potential isolate against D. citri. Biochemical determination of esterase and GST activity assay showed significant differences in activities after infection of fungi. Significantly high activity of esterase was observed by Bb-01 (27.0 unit per mg protein) on the seventh day, while Ma-11.1 and If-2.3 (16.9 and 36.3 unit per mg protein) on the third day post treatment. However, maximum GST's activity was showen by isolates Bb-08,Ma-M 2 and If-2.3 (37.6, 1.40 and 10.9 unit per mg protein) on the third day. The current investigation will help to explore the relations between the insect defense system and entomopathogenic fungi. Moreover, the determination of enzymatic activities will be useful for selecting the most pathogenic isolates.

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Selection of Beauveria bassiana sensu lato and Metarhizium anisopliae sensu lato isolates as microbial control agents against the boll weevil (Anthonomus grandis) in Argentina

Cited by 37 publications

References 39 publications

The Effect ofBeauveria brongniartiiand its Secondary Metabolites on the Detoxification Enzymes of the Pine Caterpillar,Dendrolimus tabulaeformis

The Effect ofBeauveria brongniartiiand its Secondary Metabolites on the Detoxification Enzymes of the Pine Caterpillar,Dendrolimus tabulaeformis

Bioefficacy of Alpinia galanga (Zingiberaceae) Rhizome Extracts, (<I>E</I>)<I>-p</I>-Acetoxycinnamyl Alcohol, and (<I>E</I>)<I>-p</I>-Coumaryl Alcohol Ethyl Ether Against Bactrocera dorsalis (Diptera: Tephritidae) and the Impact on Detoxification Enzyme Activities

Biochemical analysis and pathogenicity of entomopathogenic fungi to Diaphorina citri Kuwayama (Hemiptera: Liviidae)

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