Selection of a Highly Virulent Fungal Isolate, Metarhizium anisopliae Ma TB 101, for Control of Taro Beetle, Papuana uninodis (Coleoptera: Scarabaeidae)

Theunis, W.; Aloali'i, I.

doi:10.1080/09583159830252

Cited by 7 publications

(4 citation statements)

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“…LT 50 values of CLO 53 for H. philanthus at 20°C were 7 weeks at the concentration 10 6 conidia/g soil. The studies of Rath et al (1995a) and Theunis and AloaliÕi (1998) demonstrated that at 20°C the LT 50 for thirdinstar A. couloni and Papuana uninodis Dynastinae (Coleoptera: Scarabaeidae) was 3.5-4.0 weeks at the same concentration. This discrepancy may be explained by differences in origin of both the fungi and the insect.…”

Section: Discussionmentioning

confidence: 98%

Selection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus

Ansari

Vestergaard²,

Tirry

et al. 2004

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 98%

Selection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus

Ansari

Vestergaard²,

Tirry

et al. 2004

Journal of Invertebrate Pathology

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“…Metarhizium anisopliae and B. bassiana are naturally associated with SPW and have been reported to cause 70 -80% mortality (Su et al, 1988;Rana and Villacarlos, 1991;Ondiaka et al, 2008). In PNG, field infections of M. anisopliae have been reported on coconut pests (Prior and Arura, 1985), sugarcane borer (Sesamia grisescens) (Arzumanov et al, 2005) and taro beetles (Papuana uninodis) (Theunis and Aloali'i, 1998). In the Bundaberg area of Queensland, Australia, where sweet potato is cultivated commercially, natural infection by M. anisopliae has been associated with sugarcane white grubs (Antitrogus consanguineus) (Allsopp et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Screening of tropical isolates of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) for virulence to the sweet potato weevil, Cylas formicarius (Coleoptera: Brentidae)

Dotaona

Wilson

Stevens

et al. 2015

Int. J. Trop. Insect Sci.

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The sweet potato weevil (SPW), Cylas formicarius, is a serious pest of sweet potato in Australia and Papua New Guinea. Ten strains of Metarhizium sp. isolated from Australian soil samples were evaluated for their growth characteristics and screened for virulence to adult SPW under laboratory conditions. All isolates except QD62 (48.6%) had moderate to high germination (66 -97%), and all took 2 to 4 days to sporulate at 25 8C. The optimal temperature for radial growth for the majority of isolates was 30 8C, and there was a significant interaction between isolate and temperature (P , 0.05). Isolate QS155 showed the fastest radial growth at 30 8C. The internal transcribed spacer sequences showed slight variations among the isolates; however, all isolates were shown to be Metarhizium anisopliae. Isolates varied greatly in their virulence. At 10 days after inoculation (DAI) by immersion in a suspension of 1 £ 10 7 conidia/ml, 9 of the 10 isolates were virulent, causing 80-100% mortality of adult SPW. Only two isolates (QS001-6 and QS155) caused more than 50% mortality at 5 DAI. In dose-mortality bioassays, isolate QS155 had the lowest 20-day LC 50 and LC 90 values; however, there were no statistically significant differences in mortality among the three most promising isolates tested (QD66, QS001-6 and QS155). These results show that M. anisopliae isolate QS155 has potential as a microbial control agent for SPW, and that further evaluation under glasshouse and field conditions is warranted.

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“…This entomopathogenic fungus has been currently observed in several scarabaeid beetles species [31], [32], [33] including T. lusitanicus (personal observation) what it is important to assure the pathogenic capacity of the conidia formulation. We examined temporal changes in the total protein contents, proPO, PO and variation in the mortality rates of inoculated individuals submitted to diets based on acorns or cow dung.…”

Section: Introductionmentioning

confidence: 99%

Acorn Consumption Improves the Immune Response of the Dung Beetle Thorectes lusitanicus

et al. 2013

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Thorectes lusitanicus, a typically coprophagous species is also actively attracted to oak acorns, consuming, burying them, and conferring ecophysiological and reproductive advantages to both the beetle and the tree. In this study, we explored the possible relation between diet shift and the health status of T. lusitanicus using a generalist entomopathogenic fungus (Metarhizium anisopliae) as a natural pathogen. To measure the health condition and immune response of beetles, we analysed the protein content in the haemolymph, prophenoloxidase (proPO) content, phenoloxidase (PO) activity and mortality of beetles with diets based on either acorns or cow dung. Protein content, proPO levels and PO levels in the haemolymph of T. lusitanicus were found to be dependent on the type of diet. Furthermore, the beetles fed with acorns developed a more effective proPO-PO system than the beetles fed with cow dung. Furthermore, a significant decrease in mortality was observed when infected individuals were submitted to an acorn-based diet. In addition to enhancing an understanding of the relevance of dietary change to the evolutionary biology of dung beetles, these results provide a more general understanding of the ecophysiological implications of differential dietary selection in the context of fitness.

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Selection of a Highly Virulent Fungal Isolate, Metarhizium anisopliae Ma TB 101, for Control of Taro Beetle, Papuana uninodis (Coleoptera: Scarabaeidae)

Cited by 7 publications

References 0 publications

Selection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus

Selection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus

Screening of tropical isolates of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) for virulence to the sweet potato weevil, Cylas formicarius (Coleoptera: Brentidae)

Acorn Consumption Improves the Immune Response of the Dung Beetle Thorectes lusitanicus

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