Selection for virus resistance in tomato exposed to tissue culture procedures

Hanus-Fajerska, Ewa; Lech, M.; Pindel, A.; Miczyński, Kazimierz

doi:10.1007/s11738-000-0045-y

Cited by 2 publications

(1 citation statement)

References 26 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, it can complement the usual field selection. Plants tolerant to various abiotic and biotic stresses might be obtained by applying the chosen cultivation protocol with appropriate selecting agents such as NaCl (for generating salt tolerance), PEG or mannitol (for drought tolerance), trace metals/metalloids (for heavy metals/metalloids tolerance), pathogen culture filtrate, phytotoxin, or pathogen itself (for disease resistance) (Hanus-Fajerska et al 2000 ; Ashrafzzadeh and Leung 2015 ; Wiszniewska et al 2015 , 2017 ; El-Minisy et al 2016 ; Kumar et al 2016 ). Only the explants capable of long run surviving in the presence of proper agent are selected due to the induction of genetic variation among cells, tissues, and/or organs in cultured and regenerated plants (Rai et al 2001 ; Sakhanokho and Kelley 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype

Muszyńska

Hanus-Fajerska

Koźmińska

2018

Water Air Soil Pollut

Self Cite

View full text Add to dashboard Cite

In vitro techniques may provide a suitable tool for effective propagation and conservation of plant species representing various ecological niches. The elaboration of such protocols is also prerequisite for selection of heavy-metal-tolerant plant material that could be afterwards used for restoration or remediation of polluted sites. In this study, culture protocol for Gypsophila fastigiata propagation was developed. The highest multiplication coefficient, which reached 6.5, and the best growth parameters were obtained on modified MS medium supplemented with 1.0 mg L−1 2iP and 0.2 mg L−1 IAA. The obtained cultures were treated with different concentrations of lead nitrate (0.1, 0.5, and 1.0 mM Pb(NO3)2) or cadmium chloride (0.5, 2.5, and 5.0 μM CdCl2). The growth parameters, photosynthetic pigments, and phenolic compound content were examined in order to evaluate whether tested metal salts can have an adverse impact on studied culture. It was ascertained that Pb ions induced growth disturbances and contributed to shoot wither. On the contrary, the proliferative shoot cultures were established on media containing Cd ions and the multiplication coefficients and shoot length increased on all media enriched with CdCl2. Chlorophylls and carotenoid contents were negatively affected by application of 5.0 μM of cadmium; nevertheless, in shoots treated with 2.5 μM CdCl2, increased accumulation of photosynthetic pigments occurred and their amount was similar to untreated culture. Adaptation to Cd was associated with stimulation of phenolic compound synthesis. Hence, we have reported on unambiguous positive result of in vitro selection procedure to obtain vigorous shoot culture tolerant to cadmium.Electronic supplementary materialThe online version of this article (10.1007/s11270-018-3702-8) contains supplementary material, which is available to authorized users.

show abstract