Selection of Powdery Mildew and Yellow Mosaic-Resistant Greengram Plants Regenerated Through Organogenesis

Ramanathan, A.; Vidhyasekaran, P.; Rathinakumar, Luke

doi:10.1080/03235400214212

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…Generally, the most prevalent diseases of mung bean in the surveyed districts were leaf blight, leaf spot and powdery mildew. The result of this study is in agreement with(Ramanathan, et al, 2002;Srinives, 1996), who reported that most diseases of mung bean are leaf blight, leaf spot and powdery mildew Poehlman, (1991),. also stated leaf spot caused by (Cercospora canescens), powdery mildew(Erysiphe polygoni) are the most important diseases of mung bean.The intensities of diseases were varied across locations and seasons.…”

supporting

confidence: 92%

Assessment of Mung Bean (Vignaradiata) pests in the Low Land Areas of North Shewa Zone

Wondwosen

2019

IJSRST

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Mung bean known locally as “Masho” is a recent introduction in the Ethiopian pulse production and grown in few areas of the country and constrained by different biotic factors.However, the relative importance of each pest across locations has not been assessed and well profiled to sound management strategy. The objective of this study was to assess distribution and intensity of major pests on mungbean.The survey was made in 2016 and 2017 main cropping seasons following the main roads and accessible routes in each survey district (lowland areas of North Shewa Zone) and stops were made at every 5 -10 km intervals based on vehicles odometers as per mung bean fields available. Five stops were made in each mung bean field by moving “X” fashion at each stop interval using quadrants and data were collected from each. The result indicated that, Apion clavipes was most prevalent insect pest of mung bean in surveyed areas with maximum prevalence of 100%. Similarly, leaf blight and leaf spot were most prevalent diseases of mung bean in all districts. On the other hand, survey revealed that Cyperus spp. was frequently observed weed in both seasons with the highest weed density per m2. In 2017, weed Spps. Cyperus rotundus and Echinocloacolona L. were more frequently obtained weeds of mung bean in the surveyed districts with the consequent frequencies (77.42, 70.97%) and densities (16.11, 2.8 per m2). The current study indicated that a complex of pests exist on mung bean in the studied areas. Therefore, pest management strategies need to be devised.

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confidence: 92%

Assessment of Mung Bean (Vignaradiata) pests in the Low Land Areas of North Shewa Zone

Wondwosen

2019

IJSRST

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“…Biotic and abiotic inducers play important role in activating the defense genes in plants [47]. Coumarins are phytoalexins produced as a result of infection caused due to fungi in various plants [48][49][50].…”

Section: Induction Of Enzymes Of the Phenylpropanoid Pathwaymentioning

confidence: 99%

Phenyl Derivative of Pyranocoumarin Precludes Fusarium oxysporum f.sp. lycopersici Infection in Lycopersicon esculentum via Induction of Enzymes of the Phenylpropanoid Pathway

Sangeetha

Sarada

2014

Appl Biochem Biotechnol

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Binding of phenyl derivative of pyranocoumarin (PDP) modulated activity of fungal endopolygalacturonase in silico. Induced fit docking study of PDP with endopolygalacturonase (1HG8) showed a bifurcated hydrogen bond interaction with the protein at Lys 244 with a docking score of -3.6 and glide energy of -37.30 kcal/mol. Docking with endopolygalacturonase II (1CZF) resulted hydrogen bond formation with Lys 258 with a docking score of -2.3 and glide energy of -30.42 kcal/mol. It was hypothesized that this modulation favors accumulation of cell wall fragments (oligogalacturonides) which act as elicitors of plant defense responses. In order to prove the same, in vivo studies were carried out using a formulation developed from PDP (PDP 5EC) on greenhouse grown Lycopersicon esculentum L. The formulation was effective at different concentrations in reduction of seed infection, improvement of vigor and control of Fusarium oxysporum f.sp. lycopersici infection in L. esculentum. At a concentration of 2 %, PDP 5EC significant reduction in seed infection (95.83 %), improvement in seed vigor (64.31 %) and control of F. oxysporum f.sp. lycopersici infection (96.15 %) were observed. Further application of PDP 5EC to L. esculentum challenged with F. oxysporum f.sp. lycopersici significantly increased the activity of enzymes of the phenylpropanoid pathway, namely, peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), and enhanced the total phenolic content when compared to the control.

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“…Despite its known activity in the adenine derived compounds (cytokinins), adenine itself is used in tissue cultures for plant regeneration. Adenine as a potent growth promoter, when used along with 6-BAP or kinetin often proved more advantageous in organogenesis of many species like Vigna (Ayyasami et al, 2002), jojoba (Hassan et al, 2003), pear (Yancheva et al, 2006) etc.…”

Section: Regeneration Experimentsmentioning

confidence: 99%

Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

Pushyami¹,

Beena²,

Sinha³

et al. 2012

JPS

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A repeatable protocol was developed for shoot regeneration from hypocotyl explants in jute (C. capsularis) that resulted in 25% regeneration using a combination of 6-BAP (7.5 mgl-1) and adenine hemisulfate (50 mgl-1). Rapid shoot proliferation was observed on medium containing NAA (0.1 mgl-1) and 6-BAP (1 mgl-1). The elongated shoots rooted on basal MS and could be successfully acclimatized, which later developed fertile flowers and viable seeds. After establishing regeneration protocol for jute from hypocotyl explants, the possibility of its response for protoplast isolation and culture was studied. The efficiency of isolation of protoplasts from hypocotyls was influenced by conditions of seedling growth, incubation time in enzyme solution, constitution of the enzyme mixture, osmoticum and purification methods. Isolated non-vacuolated protoplasts when cultured in modified Kao's medium with 7.2 gl-l sucrose produced microcalli in about four weeks. However, these microcalli did not develop further.

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Selection of Powdery Mildew and Yellow Mosaic-Resistant Greengram Plants Regenerated Through Organogenesis

Cited by 3 publications

References 20 publications

Assessment of Mung Bean (Vignaradiata) pests in the Low Land Areas of North Shewa Zone

Assessment of Mung Bean (Vignaradiata) pests in the Low Land Areas of North Shewa Zone

Phenyl Derivative of Pyranocoumarin Precludes Fusarium oxysporum f.sp. lycopersici Infection in Lycopersicon esculentum via Induction of Enzymes of the Phenylpropanoid Pathway

Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.

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