Selection and Screening of Endorhizosphere Bacteria from Solarized Soils as Biocontrol Agents Against Verticillium dahliae of Solanaceous Hosts

Tjamos, E. C.; Tsitsigiannis, Dimitrios Ι.; Tjamos, Sotiriοs E.; Antoniou, Polymnia P.; Katinakis, Panayiotis

doi:10.1023/b:ejpp.0000010132.91241.cb

Cited by 124 publications

(83 citation statements)

References 27 publications

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“…Of the 112 isolates, five isolates were selected according to their high efficiency in the plate assay. These results have been demonstrated in previous research (Tjamos et al 2004;Zheng et al 2011).…”

Section: Discussionsupporting

confidence: 90%

Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Amini¹,

Agapoor²,

Ashengroph³

2016

Journal of Plant Protection Research

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Abstract:In this study, about 112 isolates of Streptomyces were isolated from chickpea rhizospheric soils. Among the isolated strains, five showed strong inhibitory effects against chickpea Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris in vitro using plate assay and selected for further studies. The selected strains were identified as Streptomyces spp. based on morphological and biochemical characterization as well as 16S rDNA sequences analysis. Our results assigned them to strains related to genus of Streptomyces.In vitro, antagonistic effects of Streptomyces strains against the disease were evaluated through the dual-culture method, volatile and non-volatile metabolites, siderophore, protease and chitinase production. All bacterial strains inhibited mycelial growth of the pathogen ranging from 26 to 44.2% in dual culture assay. The non-volatile extract of five of the Streptomyces strains inhibited more than 50% growth of the pathogen, whereas volatile compounds were less effective on mycelial growth inhibition (20.2 to 33.4%). The ability of the biocontrol agents to produce siderophore and protease were varied, whereas, production of chitinase was detected for all strains. Results of the greenhouse assay indicated that all biocontrol agents reduced disease severity (ranging from 38.7 to 54.8%).Accordingly, strain KS62 showed higher control efficacy (54.8%). In addition, the biomass of chickpea plants (plant height and dry weight) significantly increased in plants treated with Streptomyces strains compared to non-bacterized control. The results of this study showed that it may be possible to manage chickpea Fusarium wilt disease effectively by using Streptomyces species, as biocontrol agents. Therefore, evaluating their efficiency under field conditions is needed.

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Section: Discussionsupporting

confidence: 90%

Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Amini¹,

Agapoor²,

Ashengroph³

2016

Journal of Plant Protection Research

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“…An inhibition zone assay on PDA was then employed to screen for strains with high antagonistic potential. This assay had been used as a basic procedure in numerous screens for antagonistic agents (Ahmed Idris et al, 2007;Tjamos, 2004;Weller, 1985;Hagedorn et al, 1989;Ahmadzadeh and Sharifi-Tehrani, 2009). However, in some works there was no clear correlation between the results of this assay and in situ biological control results (Expert and Digat, 1995;Lindow, 1988;Reddy et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Shirzad¹,

Fallahzadeh-Mamaghani²,

Pazhouhandeh³

2012

The Plant Pathology Journal

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“…Successful biocontrol agents at the field scale need to satisfy all of the following criteria: (a) reduce microsclerotia in the soil bank; (b) reduce incidence of Verticillium wilt on the crop; (c) improve yields of infected plants to levels comparable with healthy plants or crops grown in fumigated soils. Although several biological agents have been identified for potential activity against V. dahliae in recent years (6,157), none have met the above criteria for the successful development of an applied field product.…”

Section: Klosterman Et Almentioning

confidence: 99%

Diversity, Pathogenicity, and Management of Verticillium Species

Klosterman

Atallah

Vallad

et al. 2009

Annu. Rev. Phytopathol.

610

564

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The genus Verticillium encompasses phytopathogenic species that cause vascular wilts of plants. In this review, we focus on Verticillium dahliae, placing emphasis on the controversy surrounding the elevation of a long-spored variant as a new species, recent advances in the analysis of compatible and incompatible interactions, highlighted by the use of strains expressing fluorescent proteins, and the genetic diversity among Verticillium spp. A synthesis of the approaches to explore genetic diversity, gene flow, and the potential for cryptic recombination is provided. Control of Verticillium wilt has relied on a panoply of chemical and nonchemical strategies, but is beset with environmental or site-specific efficacy problems. Host resistance remains the most logical choice, but is unavailable in most crops. The genetic basis of resistance to Verticillium wilt is unknown in most crops, as are the subcellular signaling mechanisms associated with Ve-mediated, race-specific resistance. Increased understanding in each of these areas promises to facilitate management of Verticillium wilts across a broad range of crops.

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Selection and Screening of Endorhizosphere Bacteria from Solarized Soils as Biocontrol Agents Against Verticillium dahliae of Solanaceous Hosts

Cited by 124 publications

References 27 publications

Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Diversity, Pathogenicity, and Management of Verticillium Species

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