Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Abstract

Abstract:In this study, about 112 isolates of Streptomyces were isolated from chickpea rhizospheric soils. Among the isolated strains, five showed strong inhibitory effects against chickpea Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris in vitro using plate assay and selected for further studies. The selected strains were identified as Streptomyces spp. based on morphological and biochemical characterization as well as 16S rDNA sequences analysis. Our results assigned them to strains related to genus of Streptomyces.In vitro, antagonistic effects of Streptomyces strains against the disease were evaluated through the dual-culture method, volatile and non-volatile metabolites, siderophore, protease and chitinase production. All bacterial strains inhibited mycelial growth of the pathogen ranging from 26 to 44.2% in dual culture assay. The non-volatile extract of five of the Streptomyces strains inhibited more than 50% growth of the pathogen, whereas volatile compounds were less effective on mycelial growth inhibition (20.2 to 33.4%). The ability of the biocontrol agents to produce siderophore and protease were varied, whereas, production of chitinase was detected for all strains. Results of the greenhouse assay indicated that all biocontrol agents reduced disease severity (ranging from 38.7 to 54.8%).Accordingly, strain KS62 showed higher control efficacy (54.8%). In addition, the biomass of chickpea plants (plant height and dry weight) significantly increased in plants treated with Streptomyces strains compared to non-bacterized control. The results of this study showed that it may be possible to manage chickpea Fusarium wilt disease effectively by using Streptomyces species, as biocontrol agents. Therefore, evaluating their efficiency under field conditions is needed.

Antagonistic effect of volatile and non-volatile compounds from Streptomyces strains on cultures of several phytopathogenic fungi

“…This was the case of A. alternata, revealing the importance of VOCs in controlling plant diseases. Amini et al (2016) also observed that the VOCs from Streptomyces species caused a higher reduction of F. oxysporum f. sp. ciceris.…”

Championing Sustainable Treatment of Oil Palm Basal Stem Rot Disease via Biological Control Agents

“…Pythium aphanidermatum on cucumber (El-Tarabily et al, 2009) and Fusarium oxysporum f. sp. ciceris on chickpea (Amini et al, 2016). However, not many reports on actinomycetes against BSR disease were documented.…”

Volatile Organic Compounds (VOCs) Produced By The Rhizosphere Actinobacteria Streptomyces Sp. and Amycolatopsis Sp. Strains and Their Antifungal Activity Against Colletotrichum Musae

“…The relative concentrations of the constituents are represented in the loadings graph (Figure 6b) by circles with three different colors, where red, dark blue and light blue are associated to the highest concentration constituents from the actinobacteria (ACTB-77), C. musae and co-culture, respectively. Figure 6b shows 3-methyl-butan-1-ol (1), 2-methyl-butan-1-ol (2), α-phellandrene (12), β-phellandrene (15), phenylethyl alcohol (24), p-mentha-1,5-dien-8-ol (29), menthol (31) and aristolochene (59) as constituents from the C. musae group with the highest relatively concentrations. Among them, compounds 1, 12, 15, 24 and 59 represent ca.…”