In-service
granular activated carbon (GAC) may transform into biological
activated carbon (BAC) and remove contaminants through both adsorption
and biodegradation, but it is difficult to determine its biodegradative
capacity. One approach to understand the GAC biodegradative capacity
is to compare the performance between unsterilized and sterilized
GAC, but the sterilization methods may not ensure effective microbial
inhibition and may affect adsorption. This study identified the 14C-glucose respiration rate as the best metric to evaluate
the effectiveness of three sterilization methods: sodium azide addition,
autoclaving, and γ irradiation. The sterilization protocols
were refined, including continuously feeding 300 mg/L of sodium azide,
three cycles of autoclaving, and 10–12 kGy of γ irradiation.
Parallel minicolumn tests were conducted to identify sodium azide
addition as the most broadly effective sterilization method with an
insignificant effect on adsorption in most cases, except for the adsorption
of anionic compounds under certain conditions. Nevertheless, this
problem was solved by decreasing the azide dosage as long as it is
still sufficient to provide effective microbial inhibition. This study
helps to develop an approach that differentiates adsorption and biodegradation
in GAC, which could be used by future studies to advance our understanding
of BAC filtration.