Selection and Characterization of Aptamers against Salmonella typhimurium Using Whole-Bacterium Systemic Evolution of Ligands by Exponential Enrichment (SELEX)

Duan, Nuo; Wu, Shijia; Chen, Xiujuan; Huang, Yukun; Xia, Yu; Ma, Xiaoyuan; Wang, Zhouping

doi:10.1021/jf400767d

Cited by 153 publications

(87 citation statements)

References 25 publications

(36 reference statements)

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“…LOD is 3 CFU/ml in bacterial suspension.Ma et al (2014) S. typhimurium whole live cellsDNASandwich-type system with fluorescent detection. LOD is 25 CFU/ml in bacterial suspension.Duan et al (2013a) S. typhimurium whole live cellsDNAImpedimetric aptasensor. LOD is 600 CFU/ml in bacterial suspension.Labib et al (2012b) and Kolovskaya et al (2013) S. enteritidis whole live cellsDNAImpedimetric biosensor.…”

Section: Antiviral Aptamersmentioning

confidence: 99%

Aptamers against pathogenic microorganisms

Davydova

Vorobjeva

Pyshnyi

et al. 2015

Critical Reviews in Microbiology

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An important current issue of modern molecular medicine and biotechnology is the search for new approaches to early diagnostic assays and adequate therapy of infectious diseases. One of the promising solutions to this problem might be a development of nucleic acid aptamers capable of interacting specifically with bacteria, protozoa, and viruses. Such aptamers can be used for the specific recognition of infectious agents as well as for blocking of their functions. The present review summarizes various modern SELEX techniques used in this field, and of several currently identified aptamers against viral particles and unicellular organisms, and their applications. The prospects of applying nucleic acid aptamers for the development of novel detection systems and antibacterial and antiviral drugs are discussed.

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Section: Antiviral Aptamersmentioning

confidence: 99%

Aptamers against pathogenic microorganisms

Davydova

Vorobjeva

Pyshnyi

et al. 2015

Critical Reviews in Microbiology

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“…sigmaaldrich.com). The aptamers used in our research were prepared in our laboratory using previously reported methods [26,27]. Bacteria aptamers were synthesized by the Shanghai Sangon Biological Science & Technology Company (Shanghai, China) (http://www.sangon.com).…”

Section: Materials and Instrumentationmentioning

confidence: 99%

Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles

et al. 2014

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We report on a method for simultaneous detection of the pathogens Vibrio parahaemolyticus and Salmonella typhimurium. It is based on dual fluorescence resonance energy transfer (FRET) from green-emitting quantum-dots (gQDs) and red-emitting quantum-dots (rQDs) as donors, and on novel amorphous carbon nanoparticles (CNPs) that act as acceptor. The gQDs were modified with an aptamer (Apt 1) recognizing V. parahaemolyticus, and the rQDs with an aptamer (Apt 2) recognizing S. typhimurium. The fluorescence of both QDs is strongly quenched in the presence of CNPs. However, on addition of the target analytes, the QDsaptamer-target complex is formed and quenching by CNPs is suppressed. The fluorescence of the QDs is linearly proportional to the concentration of the two pathogens in the range from 50 to 10 6 cfu·mL −1 , with detection limits as low as 25 cfu·mL −1 for V. parahaemolyticus, and of 35 cfu·mL −1 for S. typhimurium. The assay was applied to real food samples, and the results were consistent with the results obtained with plate counting methods. We presume that this strategy can be extended to the detection of other pathogenic bacteria and biomolecules by simply substituting the aptamer.

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“…The S. typhimurium aptamer were prepared in our laboratory. 26 The sequence of S. typhimurium aptamer is 5′-SH-ATAGGAGTCACGACGAC-CAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAG-GAAAAGATATGTGCGTCTACCTCTTGACTAAT-3′ (apt1), and 5′-Bio-ATAGGAGTCACGACGACCAGAAAGTAATGCC-CGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGC-GTCTACCTCTTGACTAAT-3′ (apt2), synthesized by the Shanghai Sangon Biological Science & Technology Company (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Magnetic Nanoparticles-based Aptasensor Using Gold Nanoparticles as Colorimetric Probes for the Detection of Salmonella typhimurium

Duan

Xu²,

et al. 2016

ANAL. SCI.

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This paper presents a sensitive and convenient visual methodology for Salmonella typhimurium detection using gold nanoparticles (AuNPs) as colorimetric probes and magnetic nanoparticles (MNPs) as concentration elements. In the protocol, the aptamers were first immobilized onto the surface of AuNPs and MNPs, respectively. Then, S. typhimurium were added into the above solution and incubated for 45 min. During the incubation, aptamer on the surface of nanoparticles could specifically bind to the target and form a MNPs-aptamer-S. typhimurium-aptamer-AuNPs sandwich structure complex. In a magnetic field, the formed complexes were easily separated from the solution, resulting in a fading of the AuNPs suspension and a decrease of the ultraviolet visible (UV/Vis) signal. The assay shows a linear response toward S. typhimurium concentration through a range of 25 to 10 5 cfu/mL, and the detection limit was improved to 10 cfu/mL. The applicability of the bioassay in real food samples was also investigated; the results were consistent with the experimental results obtained from plate-counting methods. It is believed that the developed aptasensor will broaden the application in bioassays.

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Selection and Characterization of Aptamers against Salmonella typhimurium Using Whole-Bacterium Systemic Evolution of Ligands by Exponential Enrichment (SELEX)

Cited by 153 publications

References 25 publications

Aptamers against pathogenic microorganisms

Aptamers against pathogenic microorganisms

Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles

Magnetic Nanoparticles-based Aptasensor Using Gold Nanoparticles as Colorimetric Probes for the Detection of Salmonella typhimurium

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