Selected antioxidant properties of alfalfa, radish, and white mustard sprouts biofortified with selenium

Abstract

The aim of this study was to evaluate the effect of application of two mineral selenium forms (selenite Se<sup>4+</sup> or selenate Se<sup>6+</sup>) on the accumulation of this element by alfalfa (<em>Medicago sativa</em>), radish (<em>Raphanus sativus</em> var. <em>sativus</em>), and white mustard (<em>Sinapis alba</em>) at early stages of plant development for biofortification of sprouts with selenium, and the impact of this process on selected phytochemical traits. For this purpose, selenium-biofortified sprouts were analyzed for the contents of l-ascorbic acid and anthocyanin as well as their antioxidant activity. Additionally, the concentration of selenium in the biomass was determined. It was demonstrated that the application of selenium contributed to increased bioaccumulation of the element in the sprouts, constituting an effective method for the production of selenium-biofortified food. Selenate was accumulated less efficiently than was selenite. It was found that a concentration of 20 µmol L<sup>−1</sup> Se in the form of both selenate and selenite was an optimal dose for enrichment of the sprouts with this element. Biofortification of the experimental species with selenium (20 µmol L<sup>−1</sup>) generally increased accumulation of anthocyanins but did not significantly alter the level of l-ascorbic acid and free radical scavenging activity. Therefore, it seems that consumption of selenium-biofortified sprouts can be an effective way to supplement low-selenium diets with this element.

Methods of Selenium Application Differentially Modulate Plant Growth, Selenium Accumulation and Speciation, Protein, Anthocyanins and Concentrations of Mineral Elements in Purple-Grained Wheat

“…In the present study, we showed that the increased concentration of anthocyanin in the purple-grained wheat cultivar was more effective by foliar application of Se than by soil application of Se ( Figure 6A). Similar results are obtained by Woch and Hawrylak-Nowak (2019) in alfalfa, radish, and white mustard sprouts applied with Se. In addition, we showed that the levels of total protein and total free amino acids were positively correlated with the anthocyanin concentrations in the grain of the purple-grained wheat cultivar ( Figures 6B, C), but the dry weight of grain was not.…”

Effects of selenium application on biochemical characteristics and biofortification level of kohlrabi (Brassica oleracea L. var. gongylodes) produce

“…These results are in agreement with the Se beneficial effect on ascorbic acid biosynthesis recorded in shallot [36] and broccoli sprouts [37]. Differently, no beneficial effect of Se on AA accumulation was recorded in mature broccoli [9], Indian mustard [19] and sprouts of alfalfa, radish and white mustard sprouts [38]. From the present research a significant Se beneficial effect on AA biosynthesis in kohlrabi arose, which is characteristic of this species with a decreasing intensity from Sonata F 1 to Dobrynya F 1 to White Vienna 1390.…”

Antioxidant and antimicrobial activities of White Radish

“…Table 2 displays the data collected. Studies on the antioxidant properties of white radish have been published from a variety of countries, including Thailand, Saudi Arabia, Poland, and Romania (Bors et al, 2015;Somman and Siwarungson, 2015;Woch and Hawrylak-Nowak, 2019;Noman et al, 2021). For the first time, the TAS, TOS, and OSI values of white radish tuber and aerial sections were determined in this research.…”