Seizure-induced thoracolumbar burst fractures — Not to be missed

Robichaud, Aaron; Barry, Tricia K.; Barry, Seán P.

doi:10.1016/j.ebr.2019.100352

Cited by 8 publications

(10 citation statements)

References 16 publications

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“…The instrument was initially configured to mirror previous parameters for IR-MALDESI analysis on an Orbitrap platform. 35 The initial parameters used placed the laser axis 1 mm from the electrospray emitter tip and 5 mm from the inlet capillary. Varying these measurements by up to several millimeters had only subtle effects on signal for the Triton X-100 solution; therefore, the initial positioning was retained.…”

Section: Resultsmentioning

confidence: 99%

“…IR-MALDESI utilizes an infrared laser for sampling and generates ions through interactions between the aerosol of ablated material and charged electrospray droplets. 12,13 The mechanism of ionization is very similar to ESI or DESI, where the ions are produced primarily from protonation/ deprotonation reactions. 14 IR-MALDESI has two key features that make it suitable for detection of analytes in a high-throughput fashion.…”

Section: Infrared Matrix-assisted Laser Desorption Electrospray Ionizmentioning

confidence: 99%

“…A proof-ofconcept imaging experiment was performed using an existing platform for imaging, adapted for the IM-QTOF platform. 12 A locally sourced oak leaf on the NC State campus was harvested for imaging analysis and analyzed with sequential line scans ( Figure 7A). IMS-MS spectra were extracted from the Agilent.d file and converted to .imzML format 48 as a single row of TOF scans, which was subsequently loaded with a modified version of MSiReader 1.01 49,50 that is currently under development to support IMS-MS data.…”

Section: Intact Protein Analysismentioning

confidence: 99%

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Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications

Ekelöf

Dodds

Khodjaniyazova

et al. 2020

J. Am. Soc. Mass Spectrom.

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Section: Resultsmentioning

confidence: 99%

Section: Infrared Matrix-assisted Laser Desorption Electrospray Ionizmentioning

confidence: 99%

Section: Intact Protein Analysismentioning

confidence: 99%

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Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications

Ekelöf

Dodds

Khodjaniyazova

et al. 2020

J. Am. Soc. Mass Spectrom.

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show abstract

“…An IR-MALDESI source was used for all imaging experiments adapted from previous work. 34,37,38 A single laser pulse from an IR OPO laser (Opotek, Carlsbad, CA) tuned to 2.94 μm wavelength was used at each spot to ablate the ice-covered samples. The desorbed plume was extracted and ionized by a perpendicular electrospray that carried the sample into the mass spectrometer (ThermoFisher Q Exactive Plus, Bremen, Germany).…”

Section: Sample Preparation and Ir-maldesi Msi Operationmentioning

confidence: 99%

“…[31][32][33] We have previously demonstrated MSI analysis of ARVs in hair using infrared matrix assisted laser desorption ionization (IR-MALDESI). 34,35 While we have demonstrated the detection of ARVs in hair, we have yet to investigate the longitudinal profiles of ARVs in a controlled setting. Understanding a longitudinal profile will require evaluating the profile of different observed dosing strategies, understanding dose-response relationships, and evaluating the response of adherent versus non-or remotely adherent patients.…”

mentioning

confidence: 99%

Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging of Human Hair to Characterize Longitudinal Profiles of the Antiretroviral Maraviroc for Adherence Monitoring

et al. 2019

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Here, we assess infrared matrix assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) analysis of hair as a clinical tool for monitoring patient adherence to the antiretroviral maraviroc (MVC). A custom MATLAB-based algorithm has been developed to streamline data analysis and generate longitudinal profiles of drug incorporation along the length of hair strands. Hair strands from volunteers enrolled in a directly observed therapy study were analyzed by IR-MALDESI MSI and processed using this tool to characterize the profiles of single doses and a daily dose regimen of MVC. Single dose responses were 1.7 [1.1, 2.5] mm (median [range]) wide along the length of the hair and were detected in 8 out of 12 volunteers. Daily dose profiles capturing 28 days of continuous dosing were approximately 5 times the intensity of single dose profiles and 10.5 [7.0, 13] mm wide, corresponding to 1 month of hair growth. MVC ion abundance was observed in all 12 volunteers for the daily dosing period. Daily dosing profiles were consistent with a model of MVC accumulation in hair based on linear superposition of a single dose response, indicating the potential for prediction of daily drug-taking behavior based on deconvolution of a complex longitudinal profile in hair.

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Application of a Scavenger Receptor A1-Targeted Polymeric Prodrug Platform for Lymphatic Drug Delivery in HIV

et al. 2020

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We have developed a macromolecular prodrug platform based on poly (L-lysine succinylated) (PLS) that targets scavenger receptor A1 (SR-A1), a receptor expressed by myeloid and endothelial cells. We demonstrate the selective uptake of PLS by murine macrophage, RAW 264.7 cells, which was eliminated upon cotreatment with the SR-A inhibitor polyinosinic acid (poly I). Further, we observed no uptake of PLS in a SR-A1-deficient RAW 264.7 cell line, even after 24 h incubation. In mice, PLS distributed to lymphatic organs following i.v. injection, as observed by ex vivo fluorescent imaging, and accumulated in lymph nodes following both i.v. or i.d. administration, based on immunohistochemical analysis with high-resolution microscopy. As a proof-of-concept, the HIV antiviral emtricitabine (FTC) was conjugated to the polymer's succinyl groups via ester bonds, with a drug loading of 14.2% (wt/wt). The prodrug (PLS-FTC) demonstrated controlled release properties in vitro with a release half-life of 15 h in human plasma and 29 h in esterase-inhibited plasma, indicating that drug release occurs through both enzymatic and non-enzymatic mechanisms. Upon incubation of PLS-FTC with human peripheral blood Supporting Information The Supporting Information is available free of charge via the Internet at http://pubs.acs.org SEC chromatograms of PLS-488 and PLS-Cy7.5; 1 H NMR spectra of PLS, partially capped PLS, fully capped PLS, FTC, 2-(2methoxyethoxy)ethanamine, and FTC; binding and uptake of PLS-488 in RAW 264.7 cells following co-treatment with partially capped PLS, fully capped PLS, poly C, or poly I; flow cytometry plots of RAW 264.7 and clone 1/2 cells following 24 h incubation with PLS-488; confocal images of PLS-488 uptake in RAW 264.7 and clone ½ cells; whole-body images of PLS-Cy7.5 treated mice; ex vivo images of organs harvested 6 h after PLS-Cy7.5 i.v. injection; IHC staining controls; standard curves of FTC with and without NaOH hydrolysis.

show abstract

Seizure-induced thoracolumbar burst fractures — Not to be missed

Cited by 8 publications

References 16 publications

Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications

Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications

Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging of Human Hair to Characterize Longitudinal Profiles of the Antiretroviral Maraviroc for Adherence Monitoring

Application of a Scavenger Receptor A1-Targeted Polymeric Prodrug Platform for Lymphatic Drug Delivery in HIV

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