Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications

Abstract

Because of its high degree of selectivity and chemical resolution, mass spectrometry (MS) is rapidly becoming the analytical method of choice for high-throughput evaluations and clinical diagnostics. While advances in MS resolving power have increased by an order of magnitude over the past decade, advances in sample introduction are still needed for high-throughput screening

Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: A brief review and perspective

“…Ekelof et al described the first inclusion of DTIMS to IR-MALDESI ( Figure 2F) for effective imaging. 50 Validation of the modified instrument demonstrated calculated CCS differences of less than 3.1% of previously reported values. Of note in this study, both proteins and small molecules were efficiently ionized It is important to note that this added data dimension presents significant computational challenges.…”

“…124,[126][127][128][129] and IR-MALDESI demonstrating the potential for its expanded use in the field. 43,45,46,50,83,133,134 In 2014, Jackson and coworkers utilized MALDI-DTIMS for imaging various lipid classes where distinct mobility trends for each lipid subclass were shown to correlate with lipid head group size. 132 Many recent advancements in DTIMS resolving power [135][136][137][138][139] may provide the impetus for investigators to develop further strategies for integrating DTIMS with IMS.…”

“…Ultimately, we foresee an increase in commercial software capable of handling ion mobility-IMS data in the near future, in addition to the publication and distribution of lab-built, stand-alone software. For example, the Muddiman group is currently updating MSiReader to include ion mobility support 50,146,147 while the Van de Plas 37,100,101 and Laskin 148 laboratories are each presently developing software capable of handling ion mobility-IMS data. Readily available and robust data analysis software that is reasonably easy to use will be more quickly incorporated within academic labs and enable higher quality analysis and interpretation of these complex data.…”

“…Ion mobility has been integrated with several IMS techniques including matrix‐assisted laser desorption/ionization (MALDI), 41,42 desorption electrospray ionization (DESI), 43,44 laser‐assisted electrospray ionization (LAESI), 45,46 liquid‐extraction surface analysis (LESA), 47,48 liquid‐microjunction surface sampling probe (LMJ‐SSP), 49 and infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) 50 (Figure 2). Briefly, MALDI relies on laser desorption for introduction of analytes to the mass spectrometer, where LESA, DESI, and LMJ‐SSP are electrospray‐based techniques that use solvent to desorb analytes for detection 8,51–53 .…”

Nanospray Desorption Electrospray Ionization (nano-DESI) Mass Spectrometry Imaging of Drift Time-Separated Ions

“…[31][32][33] Several ion mobility instruments have been successfully coupled with MSI techniques including MALDI, 34,35 DESI, 36,37 liquid extraction surface analysis (LESA), 38 laser desorption electrospray ionization (LAESI) 39 and infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI). 40 The advantages of such coupling include an improved molecular coverage and sensitivity, the ability to generate background-free images, and rapid isomeric separation. 25,41 Herein, we describe the design and performance of a portable high-resolution nano-DESI imaging platform coupled to a linear ion mobility quadrupole time-of-flight mass spectrometer (IM-QTOF MS), which enables imaging of drift time-separated ions.…”