2016
DOI: 10.1017/s143192761600074x
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Segmentation Method of Time-Lapse Microscopy Images with the Focus on Biocompatibility Assessment

Abstract: Biocompatibility testing of new materials is often performed in vitro by measuring the growth rate of mammalian cancer cells in time-lapse images acquired by phase contrast microscopes. The growth rate is measured by tracking cell coverage, which requires an accurate automatic segmentation method. However, cancer cells have irregular shapes that change over time, the mottled background pattern is partially visible through the cells and the images contain artifacts such as halos. We developed a novel algorithm … Show more

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Cited by 3 publications
(2 citation statements)
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“…As it is seen in phase contrast (Fig. 6 B, PhC) around the microcell is empty area that indicates to rise endocytosis and cell viability [ 35 ].
Fig.
…”
Section: Resultsmentioning
confidence: 99%
“…As it is seen in phase contrast (Fig. 6 B, PhC) around the microcell is empty area that indicates to rise endocytosis and cell viability [ 35 ].
Fig.
…”
Section: Resultsmentioning
confidence: 99%
“…It is typically performed within a controlled environment to maintain optimal culture conditions for the viability of the specimen, including temperature, humidity, and gas concentrations. Time-lapse microscopy is well documented in the literature, playing a pivotal role in the study of various aspects of cell behavior [ 51 , 52 ], morphological changes [ 53 , 54 ], and responses to drug treatment [ 55–57 ]. Notably, time-lapse microscopy removes the need for human intervention during the culture process and obviates the need for the removal of the specimen from its culture environment for visual inspection.…”
Section: Morphology-based Imagingmentioning
confidence: 99%