Seeds of Amazonian Fabaceae as a source of new lectins

Fernandes, Andréia Varmes; Ramos, Márcio V.; Gonçalves, José Francisco de Carvalho; Maranhão, Paulo A.C.; Chevreuil, Larissa Ramos; Souza, Luiz Augusto Gomes de

doi:10.1590/s1677-04202011000300008

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Em estudos com o gênero Ormosia, resultados relataram que o mesmo apresenta uma grande diversidade de compostos, tais como alcaloides quinolizidínicos altamente complexos (alcaloides Ormosia), isoflavonas, lecitinas, proantocianidinas e cerebrósidos, indicando seu potencial de aproveitamento (Fernandes et al, 2011;Pouny et al, 2014).…”

Section: Introductionunclassified

Germinação de sementes e crescimento de Ormosia arborea em diferentes temperaturas e substratos

et al. 2016

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-Olho-de-cabra species belonging to the Fabaceae family, distributed through of Atlantic rain forest and semideciduous stationary forest. Its wood is suitable for manufacture of furniture and tree recommended for urban forestry and also considered medicinal. Taking into account their potential use this work was developed with the objective of evaluates the effect of different temperatures and substrates on the germination rate and initial root growth. The seeds used in this experiment were collected in the Savannah areas, Mato Grosso do Sul state and submitted to constant temperatures of 20, 25, 30, 35 °C and alternating, 20-30 and 25-35 °C, in three substrates, sand, vermiculite and paper roll, in germination cameras. The statistical design was a completely randomized design and means were compared by Tukey test at 5%. The data indicated that the species need a period of time longer than 15 days for the formation of aerial part and that the development of its root system is faster. The obtained results show that the best temperatures to the seeds germination is 30 °C, sand, while the best seedling development was observed in the alternating temperature, 25-35 °C, also in sand, with its results statistically higher than other treatments. Key Words: Forest seeds, initial growth, Fabaceae, native species.Resumo -A espécie olho-de-cabra pertence à família Fabaceae, ocorrendo principalmente na Floresta Pluvial Atlântica e latifoliada semidecídua; sua madeira é indicada para construção de móveis e a árvore é recomendada para arborização urbana, além de ser considerada medicinal. Levando-se em consideração seu potencial de utilização, este trabalho foi desenvolvido com o objetivo de verificar o efeito de diferentes temperaturas e substratos sobre as taxas de germinação e crescimento radicular inicial. As sementes utilizadas foram coletadas em áreas de Cerrado, Mato Grosso do Sul e submetidas a seis temperaturas, constantes de 20, 25, 30 e 35 °C e alternadas, 20-30 e 25-35 °C, em três tipos de substratos, areia, vermiculita e rolo de papel, em câmaras de germinação. O delineamento estatístico utilizado foi o inteiramente casualizado e as médias comparadas pelo teste de Tukey a 5%. Os dados obtidos indicaram que a espécie necessita de um período de tempo maior que 15 dias para a formação de sua parte aérea e que o desenvolvimento do seu sistema radicular é mais rápido, com maior percentagem de germinação e vigor de sementes na temperatura de 30 °C, substrato areia. O melhor desenvolvimento das plântulas foi observado na temperatura alternada de 25-35 °C, também no substrato areia, sendo seus resultados estatisticamente superiores aos demais tratamentos. palavras chave: crescimento inicial, espécies nativas, Fabaceae, sementes Florestais.Resumen -La especie "olho-de-cabra", perteneciente a la familia de las Fabáceas, es típica de las selvas pluviales atlánticas y los bosques estacionales semidecíduos. Su madera es apropiada para la construcción de muebles y el árbol es recomendado para forestaciones urbanas, t...

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Section: Introductionunclassified

Germinação de sementes e crescimento de Ormosia arborea em diferentes temperaturas e substratos

et al. 2016

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“…Furthermore, leguminous trees in the Amazon have been reported as other important source of protein molecules, such as lectins (Fernandes et al 2011). Thus, this work was aimed at detecting and characterizing partially these proteins in tissue storage of seeds from Parkia pendula, P. discolor, P. multijuga and P. nitida.…”

Section: Introductionmentioning

confidence: 99%

Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

Chevreuil

Gonçalves

Calderon³

et al. 2014

Hoehnea

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-(Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)). Leguminous seeds (Fabaceae) have a high content of inhibitors of which serine protease inhibitors are the most widely studied. However, there are only a few studies related to the investigation of these proteins in tree species belonging to the Amazon flora. The protein content presented in seeds of four Amazonian Leguminosae species, Parkia pendula, P. discolor, P. multijuga and P. Nitida, was extracted by using NaCl 0.15 mol L -1 and then partially fractionated by using affinity chromatography performed on a trypsinSepharose 4B. These inhibitors presented different affinities between trypsin and chymotrypsin serine proteases, showing a higher inhibition to trypsin compared to chymotrypsin, except for P. nitida, which showed high inhibition against both enzymes. The SDS-PAGE analysis showed that the species from Parkia genus have a main band corresponding to partially purified trypsin inhibitors. The apparent molecular mass inhibitors (approximately 13-18 kDa) and the high specificity for trypsin suggest the occurrence of Bowman-Birk and Kunitz type inhibitors. Keywords: Amazonia, Bowman-Birk inhibitor, Kunitz inhibitor, leguminous seeds, serine protease RESUMO -(Purificação parcial de inibidores de tripsina de sementes de Parkia (Fabaceae)). Sementes de leguminosas (Fabaceae) apresentam alto conteúdo de inibidores, incluindo os inibidores de serinoproteinases que têm sido extensivamente estudados. Todavia, poucos estudos foram realizados quanto à investigação dessas proteínas em espécies arbóreas pertencentes à flora amazônica. As proteínas presentes nas sementes de quatro espécies de leguminosas da Amazônia, Parkia pendula, P. discolor, P. multijuga e P. nitida, foram obtidas pela extração usando NaCl 0.15 mol L -1 e, parcialmente purificadas usando a cromatografia de afinidade em tripsina-Sepharose 4B. Os inibidores exibiram afinidades diferentes entre a tripsina e a quimotripsina, exceto para P. nitida, a qual apresentou alta inibição contra as duas enzimas. A análise em SDS-PAGE mostrou que as espécies do gênero Parkia contém uma banda principal correspondendo aos inibidores de tripsina parcialmente purificados. As massas moleculares aparentes determinadas para os inibidores (aproximadamente, 13 a 18 kDa) e a alta especificidade pela tripsina sugerem a ocorrência de inibidores do tipo Bowman-Birk e Kunitz. Palavras-chave: Amazônia, Inibidor Bowman-Birk, Inibidor Kunitz, sementes de leguminosas, serinoproteinase 1.

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“…The tree is distributed throughout the states of Amazonas, Pará, Roraima, and Mato Grosso in Brazil, where it is popularly known as "tento" or "olho de cabra" (Campos Filho and Sartorelli, 2015). Ormosia sp has a diversity of chemical compounds important for the industry such as alkaloids, isoflavones, lecithins, and proanthocyanidins (Fernandes et al, 2011;Pouny et al, 2014). It also presents a potential for use in landscape projects, recovery of degraded areas, and handicrafts (Carneiro et al, 1998;Lorenzi, 2002;Frausin et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Relationships between substrate and the mobilization of reserve with temperature during seed germination of Ormosia coarctata Jack.

et al. 2020

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Seed germination studies provide essential information for biodiversity conservation and ecological restoration programs. This work aimed to investigate the relationship between the substrates and the mobilization of reserves during germination of Ormosia coarctata seeds under different temperatures. Samples were collected every 48 h for up to 240 h for quantification of lipids, soluble sugars, starch, and soluble proteins. The optimum temperature range for germination was 25 to 35 °C. The highest germination percentages were obtained using sand or paper roll. Carbohydrate, lipid, and protein contents decreased during germination, regardless of temperature.

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Seeds of Amazonian Fabaceae as a source of new lectins

Cited by 7 publications

References 22 publications

Germinação de sementes e crescimento de Ormosia arborea em diferentes temperaturas e substratos

Germinação de sementes e crescimento de Ormosia arborea em diferentes temperaturas e substratos

Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

Relationships between substrate and the mobilization of reserve with temperature during seed germination of Ormosia coarctata Jack.

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