Seedling imaging analysis and traditional tests to assess okra seed vigor

Abstract

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

Use of the software Seed Vigor Imaging System (SVIS®) for assessing vigor of carrot seeds

“…Similar behavior was observed by Kikuti and Marcos Filho (2013) and Marcos Filho et al, 2006 studying okra seeds and soybean seeds, respectively. Accord- ing to the authors, this behavior was due to uniform germination of the seeds which is related to the high vigor of the same.…”

“…Seed vigor can be evaluated by the physiological, biochemical and stress tolerance tests which are commonly used for carrot and other species (Pereira et al, 2008;Chiquito et al, 2012;Kikuti and Marcos Filho, 2013). Computerized image analysis of seedlings has been used for seed vigor evaluation in several species; these are non-destructive methods and can provide objective information in a short period of time with less human interference (McCormac et al, 1990;Marcos Filho et al, 2006).…”

“…The process involves scanning the seedlings and then generating vigor, growth and uniformity indexes. Further studies were conducted using this technique and successfully evaluated seed vigor, for crops such as soybean seeds (Hoffmaster et al, 2003), corn (Hoffmaster et al, 2005), melon (Marcos-Filho et al, 2006), peanut (Marchi et al, 2011), cucumber (Chiquito et al, 2012) and okra (Kikuti and Marcos Filho, 2013).…”

Performance of Bell Pepper Seeds in Response to Drum Priming with Addition of 24-Epibrassinolide

“…Initial seedling growth. Seed Vigor Imaging System software was used to verify the initial seedling growth (Hoffmaster et al, 2005;Kikuti and Marcos-Filho, 2013). Four replications of 25 seeds were used for each seed lot.…”

Seedling image analysis and traditional tests to evaluate onion seed vigor

“…After that, Sako et al (2001) developed an automated system for evaluating the vigor of lettuce seeds through computerized seedling analysis, the Seed Vigor Imaging System (SVIS ® ), which supplies information such as seedling length and indexes of vigor, growth, and uniformity of growth. This software has already been successfully tested on other species, such as melon (Marcos-Filho et al, 2006), soybean (MarcosFilho et al, 2009), maize (Mondo et al, 2011), sweet corn (Alvarenga et al, 2012), cucumber (Chiquito et al, 2012), rattlepod (Silva et al, 2012), okra (Kikuti and Marcos-Filho, 2013), lettuce (Kikuti and Marcos-Filho, 2012), dry edible bean (Gomes Junior et al, 2014), eggplant (Silva and Cícero, 2014a), and tomato (Silva and Cícero, 2014b).…”