Seedless mechanism of a new mandarin cultivar ‘Wuzishatangju’ (Citrus reticulata Blanco)

Ye, Weijia; Qin, Yonghua; Ye, Zixing; Silva, Jaime A. Teixeira da; Lingxiao, Zhang; Wu, Xiaoying; Lin, Shunquan; Hu, Guibing

doi:10.1016/j.plantsci.2009.03.005

Cited by 61 publications

(45 citation statements)

References 25 publications

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“…Seedlessness is important in relation to fruit quality, and the production of fruits with few or no seeds has steadily increased (Ye et al, 2009). Seedlessness in citrus mandarin cultivars can be induced by many factors, and many seedless citrus cultivars have been originated from the bud mutation of seedy cultivars (Yamamoto et al, 1995;Shen, 1997;Vardi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Murcott seedless: influence of gamma irradiation on citrus production and fruit quality

Bermejo

Pardo

Caño

2012

Span. j. agric. res.

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A Seedlessness is an important economic trait relating to fruit quality, and gamma irradiation is a common technique used to obtain seedless citrus fruits. Herein, we report a study of new seedless 'Murcott' mandarin clones obtained by bud irradiation from the self-compatible not parthenocarpic 'Murcott' mandarin. All irradiated clones examined presented lower seed numbers (from 0.23 to 2.47 seeds per fruit) and reduced pollen germination (from 1.40% to 8.55%) whereas the wild-type 'Murcott' showed an average number of 9.03 seeds per fruit and a pollen germination value of 47.15%. Fruit quality and nutritional bio-components were affected differently; some clones presented no changes compared to the control 'Murcott' mandarin, while other clones showed significant differences. High-performance liquid chromatographic methods were used to identify and quantify of these compounds, using photodiode array, mass and refractive index detectors. Our results indicated high contents in natural antioxidants as vitamin C (from 20.13 to 25.73 mg/100 mL) and phenolic compounds, as flavonoids, in these citrus varieties cultived under the Mediterranean climate. Some of these clones, which ripen late in the season and whose fruit quality is maintained or improved, are in the process of registration. In conclusion, budwood irradiation is a suitable technique to improve cultivars, produce seedless cultivars, adjust ripening time or raise the content of health-promoting compounds. Also this study investigates the influence of temperature during flowering on the number of seeds formed. Findings indicate that low temperatures during flower formation decreased pollen germination and seed number.Additional key words: citrus clones; mandarin; nutritional bio-components; pollen germination; temperature. ResumenMurcott sin semillas: influencia de la irradiación gamma sobre la producción de cítricos y la calidad de la fruta El carácter ''sin semillas'' es un rasgo importante relacionado con la calidad de la fruta, y la inducción de mutaciones mediante irradiaciones gamma es una técnica común utilizada para obtener frutos sin semillas de cítricos. Presentamos aquí el estudio realizado en los nuevos clones que hemos obtenido mediante irradiación, a partir de la variedad autocompatible y no partenocárpica 'Murcott'. Todos los clones irradiados examinados presentaron menor número de semillas (de 0,23 a 2,47 semillas por fruto) y reducción de la germinación del polen (de 1,40% a 8,55%), mientras que la mandarina 'Murcott' control mostró 9,03 semillas por fruto y 47,15% de germinación del polen. La calidad de la fruta y el contenido en compuestos bioactivos y nutricionales se vieron afectados de manera diferente, algunos clones no presentaron cambios con respecto al control, mientras que otros clones sí mostraron diferencias significativas. Nuestros resultados indican un alto contenido en antioxidantes naturales como la vitamina C (20,13 a 25,73 mg/100 mL) y compuestos fenólicos, como flavonoides, en estas variedades de cítricos cultivad...

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Section: Introductionmentioning

confidence: 99%

“…and wampee (Clausena lansium Lour.) (Yamamoto et al, 1995;Raza et al, 2003;Ye et al, 2009). Among citrus cultivars, mutation breeding has been used to improve sweet orange (Citrus sinensis (L.) Osb.…”

Section: Introductionmentioning

confidence: 99%

Murcott seedless: influence of gamma irradiation on citrus production and fruit quality

Bermejo

Pardo

Caño

2012

Span. j. agric. res.

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“…Even fruits with no viable seeds should be considered seedless (Varoquaux et al, 2000). Absence of seeds in citrus fruit is a pleasing trait to fresh fruit consumers and a needed attribute for the juice industry (Hu et al, 2007;Ye at al., 2009). Some cultivars were selected and are of great value because of their seedlessness.…”

Section: Introductionmentioning

confidence: 99%

‘URS Campestre’ seedless orange: a new mutant with female sterility

Bender

Santos

Guerra

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Seedlessness in fruit is a trait that is much sought after by juice making industries.Close to the city of São Sebastião do Caí, in the state of Rio Grande do Sul (RS), Brazil, a new mutant orange originating from natural mutation was identified and selected as a seedless material. To determine the mechanisms involved in the absence of seeds, the reproductive structures of this new mutant by comparison with a Valencia sweet orange as control, a cultivar with a profusion of seeds, was analyzed in terms of meiotic behavior, meiotic index, pollen viability, in vitro germination, and ovule features to determine the grounds for seed absence.Other morphological analyzes allowed for visualizing the structures of normal appearance and size in both cultivars. Meiotic analysis identified chromosome normal pairing with a predominance of bivalents at diakinesis and metaphase 1. URS Campestre flowers at different developmental stages had anthers and ovaries whose dimensions are typical while pollen grain analysis pointed to a standard developmental pattern, normal meiosis, high viability (84 %) and elevated in vitro pollen tube germination rates (63 %). The cv. Valencia and URS Campestre ovules had a similar shape and morphology, sharing an anatropous orientation, and two integuments. In the internal ovule analyses of Valencia sweet oranges, normal embryo sac cells were identified: presence of one egg cell and two synergids, three antipodes and a bigger and central cell containing two polar nuclei. However, the analysis of ovules from URS Campestre reveals an apparent senescence or non-formation of an embryo sac, where only a few highly stained and collapsed cells could be identified. These results led to the conclusion that female sterility in URS Campestre, with a total absence of a female gametophyte, is the limiting factor for fertilization and seed production.

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“…This may be addressed by using conventional breeding methods, nonconventional breeding approaches and molecular techniques (Raza et al, 2003;Vardi et al, 2008). Conventional methods of breeding seedless Citrus cultivars through hybridization have been exploited successfully using diploid parents with parthenocarpy, male sterility (MS) (Iwamasa, 1966;Sykes and Lewis, 1996), self-incompatibility, and ovule and embryo abortion (Ye et al, 2009;Yoshida et al, 2005). Breeding triploid seedless Citrus cultivars producing highly sterile pollen and embryo sac has also been developed through hybridization of tetraploid and diploid cultivars Cameron, 1980, 1985).…”

Section: Introductionmentioning

confidence: 99%

Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus

Dewi

Wakana

Tanimoto

et al. 2013

J. Japan. Soc. Hort. Sci.

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In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and 'Kiyomi') were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with 'Sudachi' as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of 'Yuzu', lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while 'Sudachi' with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R 1 and two complementary genes R 2 and R 3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R 3 is located downstream from R 2 , and gene R 1 is epistatic to R 2 and R 3 genes. Genotypes (S)r 1 r 1 r 2 r 2 --are responsible for undeveloped anthers while genotypes (S)r 1 r 1 R 2 -r 3 r 3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.

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Seedless mechanism of a new mandarin cultivar ‘Wuzishatangju’ (Citrus reticulata Blanco)

Cited by 61 publications

References 25 publications

Murcott seedless: influence of gamma irradiation on citrus production and fruit quality

Murcott seedless: influence of gamma irradiation on citrus production and fruit quality

‘URS Campestre’ seedless orange: a new mutant with female sterility

Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus

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