Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa

Loarca, Daniel; Díaz, Dánae; Quezada, Héctor; Guzmán-Ortiz, Ana Laura; Rebollar-Ruiz, Abril; Presas, Ana María Fernández; Ramírez-Peris, Jimena; Franco-Cendejas, Rafael; Maeda, Takeyasu; Wood, Thomas K.; García‐Contreras, Rodolfo

doi:10.3389/fmicb.2019.02322

Cited by 9 publications

(3 citation statements)

References 24 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracellular protease was quantified using aliquots obtained from the supernatants of LB overnight cultures by determining the hydrolysis of azocasein following the procedures described in ( Loarca et al., 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Resistance against two lytic phage variants attenuates virulence and antibiotic resistance in Pseudomonas aeruginosa

García-Cruz,

Rebollar-Juarez,

Limones-Martinez

et al. 2024

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

BackgroundBacteriophage therapy is becoming part of mainstream Western medicine since antibiotics of clinical use tend to fail. It involves applying lytic bacteriophages that self-replicate and induce cell lysis, thus killing their hosts. Nevertheless, bacterial killing promotes the selection of resistant clones which sometimes may exhibit a decrease in bacterial virulence or antibiotic resistance.MethodsIn this work, we studied the Pseudomonas aeruginosa lytic phage φDCL-PA6 and its variant φDCL-PA6α. Additionally, we characterized and evaluated the production of virulence factors and the virulence in a Galleria mellonella model of resistant mutants against each phage for PA14 and two clinical strains.ResultsPhage φDCL-PA6α differs from the original by only two amino acids: one in the baseplate wedge subunit and another in the tail fiber protein. According to genomic data and cross-resistance experiments, these changes may promote the change of the phage receptor from the O-antigen to the core lipopolysaccharide. Interestingly, the host range of the two phages differs as determined against the Pseudomonas aeruginosa reference strains PA14 and PAO1 and against nine multidrug-resistant isolates from ventilator associated pneumonia.ConclusionsWe show as well that phage resistance impacts virulence factor production. Specifically, phage resistance led to decreased biofilm formation, swarming, and type III secretion; therefore, the virulence towards Galleria mellonella was dramatically attenuated. Furthermore, antibiotic resistance decreased for one clinical strain. Our study highlights important potential advantages of phage therapy’s evolutionary impact that may be exploited to generate robust therapy schemes.

show abstract

Section: Methodsmentioning

confidence: 99%

Resistance against two lytic phage variants attenuates virulence and antibiotic resistance in Pseudomonas aeruginosa

García-Cruz,

Rebollar-Juarez,

Limones-Martinez

et al. 2024

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Transferring into quartz cells, the samples were read at 595 nm. 19,20 Determination of alginate. 1000 µl were taken from the stock flasks.…”

Section: Determination Of Pyocyaninmentioning

confidence: 99%

Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors inPseudomonas aeruginosa

González,

García Contreras,

García Valdés

et al. 2024

Preprint

View full text Add to dashboard Cite

ObjetiveTo evaluate the inhibitory effect of Quorum Sensing-dependent virulence factors inPseudomonas aeruginosaby 2 molecules; curcumin and 5-bromoindole-3-carboxyaldehyde.MethodsFor the tests, 3 strains ofP. aeruginosawere used; Pa01, Pa14 and ATCC 27853. The strains were reseeded in LB medium boxes for 12 hours at 37°. ON cultures of each strain were inoculated in LB broth, grown for 24 hours at 37°. Subsequently, new cultures of the strains were inoculated at starting from the ON cultures with an initial O.D 600 nm of approximately 0.0125, the following concentrations of the molecules were evaluated with these cultures: curcumin (1 and 5 μg/ml) and 5-bromoindole-3-carboxyaldehyde (30, 50 and 100 μg/ml). The factors were read virulence with their respective wavelength; protease (595 nm), elastase (495 nm), pyocyanin (520 nm) and alginate (530 nm).Results5-bromoindole-3-carboxyaldehyde significantly inhibited pyocyanin production in Pa01 (92.5% at 100 μg/ml), elastase in Pa01 (79.85% at 100 μg/ml), protease in Pa01 (92.8% at 50 μg/ml), and alginate in ATCC 27853 (76.16% at 100 μg/ml). Curcumin showed substantial inhibition of pyocyanin in ATCC 27853 (100% at 5 μg/ml), elastase in Pa14 (56.19% at 5 μg/ml), protease in Pa01 (87.04% at 5 μg/ml), and alginate in Pa01 (48.26% at 5 μg/ml).ConclusionsCurcumin and 5-bromoindole-3-carboxyaldehyde demonstrated effective inhibition of QS-dependent virulence factors inP. aeruginosa. These findings suggest the potential of these compounds as anti-virulence agents, providing a promising alternative to conventional antibiotics in combatingP. aeruginosainfections.

show abstract

“…For example, Vibrio cholerae produces chitinase to break down chitin into oligosaccharides, which supports growth (Drescher et al, 2014 ). In Pseudomonas aeruginosa , quorum sensing and other stationary phase regulators control the production of extracellular proteases that have a role in virulence (Loarca et al, 2019 ; Sandoz et al, 2007 ) and growth on polymeric nutrient sources (Li & Lee, 2019 ). Another example is the Trichoderma reesei cellulases, which are involved in depolymerising plant cell wall polysaccharides to access carbon (Martinez et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis extracellular protease production incurs a context‐dependent cost

Rosazza

Eigentler

Earl

et al. 2023

Molecular Microbiology

View full text Add to dashboard Cite

Microbes encounter a wide range of polymeric nutrient sources in various environmental settings, which require processing to facilitate growth. Bacillus subtilis, a bacterium found in the rhizosphere and broader soil environment, is highly adaptable and resilient due to its ability to utilise diverse sources of carbon and nitrogen. Here, we explore the role of extracellular proteases in supporting growth and assess the cost associated with their production. We provide evidence of the essentiality of extracellular proteases when B. subtilis is provided with an abundant, but polymeric nutrient source and demonstrate the extracellular proteases as a shared public good that can operate over a distance. We show that B. subtilis is subjected to a public good dilemma, specifically in the context of growth sustained by the digestion of a polymeric food source. Furthermore, using mathematical simulations, we uncover that this selectively enforced dilemma is driven by the relative cost of producing the public good. Collectively, our findings reveal how bacteria can survive in environments that vary in terms of immediate nutrient accessibility and the consequent impact on the population composition. These findings enhance our fundamental understanding of how bacteria respond to diverse environments, which has importance to contexts ranging from survival in the soil to infection and pathogenesis scenarios.

show abstract

Seeding Public Goods Is Essential for Maintaining Cooperation in Pseudomonas aeruginosa

Cited by 9 publications

References 24 publications

Resistance against two lytic phage variants attenuates virulence and antibiotic resistance in Pseudomonas aeruginosa

Resistance against two lytic phage variants attenuates virulence and antibiotic resistance in Pseudomonas aeruginosa

Effect of curcumin and 5-bromoindole-3-carboxyaldehyde on the inhibition of Quorum sensig-dependent virulence factors inPseudomonas aeruginosa

Bacillus subtilis extracellular protease production incurs a context‐dependent cost

Contact Info

Product

Resources

About