Seed treatment with 5-azacytidine reduces ageing-induced damage in onion seeds

Yalamalle, V. R.; Ithape, Dinesh Manohar; Kumar, Ashok; Bhagat, Kiran; Ghosh, S. K.; Singh, Mayank

doi:10.15258/sst.2020.48.3.09

Cited by 5 publications

(4 citation statements)

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“…Seed ageing leads to accumulation of ROS, which damages the cellular membrane. The degraded membranes leach the solutes leading to loss of seed viability and vigour (Kurek et al, 2019;Yalamalle et al, 2020). In the present study, there was an increase in the EC with the seed age.…”

Section: Resultssupporting

confidence: 47%

Loss of seed viability in onion (Allium cepa L.) in relation to degradation of lipids during storage

Yalamalle

Gaikwad

Ithape

et al. 2020

JANS

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Onion seeds have one of the lowest longevity among the cultivated crops as it loses viability within a year. The unmarketable seeds have no reported food value or feed value. Onion seeds contain considerable oil content which has potential industrial utility. The present study reports the changes in the quality of seed oil extracted from seeds stored for one year (2019-2020), after which the seeds were unmarketable due to low germination. The physico-chemical properties of seed oil were studied in four onion (Allium cepa L.) varieties (Bhima Safed, Bhima Super, Bhima Dark Red and Bhima Shakti) by standard analytical test. The per cent oil content varied from 12.94% - 16.02% and storage resulted in 0.64% loss in seed oil content. The mean seed germination in freshly harvested seeds was 80.50%, which declined to 52.33% after one year of ambient storage. The electrical conductivity, which measures the membrane integrity increased by 108.60% in one-year-old seeds. The physico-chemical properties of the seed oil deteriorated with the age of the seed. In comparison to the control, the acid values and peroxide values of the seed oil increased by 51.11% and 12.90% respectively, while saponification value decreased by 36.18%. Among the varieties, Bhima Dark Red had the highest degradation in the seed storability and vigour, which also had the highest deterioration in the lipid quality. The present study reports for the first time the degradation of seed lipids during ageing in onion which might be contributing to the poor storability of onion seeds.

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Section: Resultssupporting

confidence: 47%

Loss of seed viability in onion (Allium cepa L.) in relation to degradation of lipids during storage

Yalamalle

Gaikwad

Ithape

et al. 2020

JANS

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“…As a result, enzymes in the seeds are deactivated, and the germinability of the seed is gradually lost. Seed aging reduces the activities of enzymes associated with seed germination, such as SOD, CAT, and POD, thereby hindering germination [68]. The present experiment revealed that SOD, CAT, and POD activities decreased in aged wheat seeds.…”

Section: Discussionsupporting

confidence: 44%

Neodymium Nitrate Improves the Germination of Aged Wheat Seeds by Increasing Soluble Substances and Activating Antioxidative and Metabolic Enzymes in Seeds

Hu,

Zhou,

Zhu

et al. 2023

Agronomy

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Seeds stored for a prolonged period are subject to aging and a reduction in germination potential (GP), which will negatively affect seed sales. Rare-earth elements have a synergistic effect on the improvement of seed GP. In this study, we examined the effects of neodymium on biochemical components, the antioxidant protective system, and metabolism-related enzymes during germination of naturally and artificially aged seeds of three wheat cultivars. Seed germination indices, biochemical substance contents, and enzyme activities decreased after seed aging. Soaking seeds in a neodymium nitrate solution revived aged wheat seeds at an optimal concentration of 20 µmol/L for 8 h. Soaking in a neodymium nitrate solution increased the GP4 (by 2.25–60.9%), germination index (by 1.69–29.2%), and vigor index (by 3.36–18.7%) of aged seeds. Compared with non-soaked seeds, soaking significantly changed the contents of biochemical substances, and the activities of antioxidant protective enzymes and metabolic enzymes in seedlings were increased. Soaking with neodymium may revive aged seeds by regulating the synthesis of soluble sugars, soluble proteins, chlorophyll, and carotenoids and decomposing malondialdehyde in the germinating seed. Root dehydrogenase and amylase showed different responses to the aging modes. The differential responses of root dehydrogenase and amylase may reflect differences in the resistance of enzymes to long-term mild seed aging and short-term severe environmental aging.

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“…Consequently, there is a huge need for new biochemical or molecular markers of seed viability that improve the process of monitoring plant material during storage in gene banks and to help with the understanding of the concomitant aging process occurring in seeds. These studies have developed rapidly over the last decade and substantiate the importance of volatile organic compounds [ 94 , 95 , 98 ], proteomic studies [ 191 ], RNA integrity [ 192 , 193 , 194 , 195 , 196 ] and DNA damage markers or epigenetic marks [ 96 , 97 , 99 , 190 , 197 , 198 , 199 , 200 ] that are highly promising and should be more widely explored.…”

Section: Discussionmentioning

confidence: 99%

Choosing the Right Path for the Successful Storage of Seeds

Trusiak

Plitta-Michalak

Michalak

2022

Plants

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Seeds are the most commonly used source of storage material to preserve the genetic diversity of plants. However, prior to the deposition of seeds in gene banks, several questions need to be addressed. Here, we illustrate the scheme that can be used to ensure that the most optimal conditions are identified to enable the long-term storage of seeds. The main questions that need to be answered pertain to the production of viable seeds by plants, the availability of proper protocols for dormancy alleviation and germination, seed tolerance to desiccation and cold storage at −20 °C. Finally, it is very important to fully understand the capability or lack thereof for seeds or their explants to tolerate cryogenic conditions. The proper storage regimes for orthodox, intermediate and recalcitrant seeds are discussed.

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Seed treatment with 5-azacytidine reduces ageing-induced damage in onion seeds

Cited by 5 publications

References 0 publications

Loss of seed viability in onion (Allium cepa L.) in relation to degradation of lipids during storage

Loss of seed viability in onion (Allium cepa L.) in relation to degradation of lipids during storage

Neodymium Nitrate Improves the Germination of Aged Wheat Seeds by Increasing Soluble Substances and Activating Antioxidative and Metabolic Enzymes in Seeds

Choosing the Right Path for the Successful Storage of Seeds

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