Seed protein yield from someCrotalaria spp andin vitro nutritional quality of that fromC. juncea

Pandey, V. N.; Srivastava, Anoop Kumar

doi:10.1007/bf01104142

Cited by 10 publications

(4 citation statements)

References 4 publications

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“…They are used as a source of fibers, silage, and green manure for nitrogen fix-ation (Dempsey 1975;Ramos et al 2001); as cover crops for control of weeds, nematodes, and soil erosion; and as ornamentals (Miller 1967). Some Crotalaria species can be used as forage for horses and cattle owing to the large amounts of water-soluble gums and proteins in their seeds (Purseglove 1981;Pandey and Srivastava 1990). Despite this commercial value, the genetic diversity of Crotalaria species germplasm has not been assessed.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Crotalaria germplasm assessed through phylogenetic analysis of EST-SSR markers

Wang¹,

Mosjidis²,

Morris³

et al. 2006

Genome

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The genetic diversity of the genus Crotalaria is unknown even though many species in this genus are economically valuable. We report the first study in which polymorphic expressed sequence tag-simple sequence repeat (EST-SSR) markers derived from Medicago and soybean were used to assess the genetic diversity of the Crotalaria germplasm collection. This collection consisted of 26 accessions representing 4 morphologically characterized species. Phylogenetic analysis partitioned accessions into 4 main groups generally along species lines and revealed that 2 accessions were incorrectly identified as Crotalaria juncea and Crotalaria spectabilis instead of Crotalaria retusa. Morphological re-examination confirmed that these 2 accessions were misclassified during curation or conservation and were indeed C. retusa. Some amplicons from Crotalaria were sequenced and their sequences showed a high similarity (89% sequence identity) to Medicago truncatula from which the EST-SSR primers were designed; however, the SSRs were completely deleted in Crotalaria. Highly distinguishing markers or more sequences are required to further classify accessions within C. juncea.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of Crotalaria germplasm assessed through phylogenetic analysis of EST-SSR markers

Wang¹,

Mosjidis²,

Morris³

et al. 2006

Genome

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“…Because of inadequate supplies and shortage of food proteins, there has been a constant search of unconventional legumes as new protein sources for use as both functional food ingredients and nutritional supplements (Tjahjadi et al, 1988;Pandey and Sirvastava, 1990;Morrow, 1991;Onweluzo et al, 1994). Onweluzo et al (1994) have reported the potential application of some lesser known legume seed fractions from the developing countries as functional food ingredients.…”

Section: Introductionmentioning

confidence: 99%

Functional Properties of Protein Concentrates from Three Chinese Indigenous Legume Seeds

Chau

Cheung

Wong

1997

J. Agric. Food Chem.

178

134

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The functional properties of the protein concentrates (PCs) from Phaseolus angularis, Phaseolus calcaratus, and Dolichos lablab seeds were investigated and compared with those of soybean PC. The minimum nitrogen solubilities of P. angularis and P. calcaratus PCs were at pH 5, while that of D. lablab PC was at pH 4. These three PCs had lower viscosities, similar emulsifying activities and emulsion stabilities, and higher water- and oil-holding capacities and foam capacities than soybean PC. Their minimum emulsifying activities, minimum foam capacities, and maximum foam stabilities were at pH 4. Moreover, emulsion stabilities of these three PCs were high (above 93.2%) over the pH range of 2−10. Keywords: Phaseolus angularis; Phaseolus calcaratus; Dolichos lablab; functional property; protein concentrate

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“…Species of this genus are wide spread throughout tropical, subtropical and to lesser extent temperate countries. It is used as forage for cattle owing to the large amounts of water soluble gums and proteins in their seeds (Pandey and Srivastava 1990). Although there are few reports of regeneration in vitro earlier, (Ohara et al 2000) the viability of transgenics of this plant has not been exploited as it is a legume.…”

Section: Introductionmentioning

confidence: 99%

Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics

Rao¹,

Agrawal²,

Mahmood

et al. 2011

Physiol Mol Biol Plants

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A transformation system which is free of in vitro plant regeneration following Agrobacterium infection is established for the forage legume, Sunnhemp (Crotalaria juncea L.) where in the entire embryo axis of the germinating seed was used as the target tissue for transformation. After standardization of transformation conditions, the cotyledonary node of the embryo axis was infected with Agrobacterium host LBA 4404 harboring the recombinant vector pCAMBIA 2301. The bivalent 1D gene of the two major foot and mouth disease virus (FMDV) serotypes 'O' and 'A 22 ' and the neomycin phosphotransferase (nptII) gene were used as the markers for optimization of the protocol. The embryo axes were pricked randomly on the cotyledonary node and co-cultivated with Agrobacterium. The germlings were then allowed to grow under standard growth room conditions in to mature fertile plants. 60 T 0 plants were established from 3 separate experiments. Three hundred seeds from the 60 T 0 plants were sown to raise the T 1 generation of which 180 were analyzed for integration of bivalent FMDV gene 1D "O" and "A 22 " and the nptII gene. Eighteen out of these 180 plants amplified both the marker genes. Two independent transgenic lines 24 and 37, showed elevated levels of expression of 12 μg and 8 μg (per gm of fresh leaf) of the bivalent ID antigen "O" and "A 22 " . The results showed that the transformation efficiency was 3 %. To the best of our knowledge, this is the first successful attempt of Agrobacterium tumefaciens mediated transformation of Sunnhemp. The protocol can generate whole plant transformants with relative ease and should be compatible to all genotypes of Sunnhemp.

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Seed protein yield from someCrotalaria spp andin vitro nutritional quality of that fromC. juncea

Cited by 10 publications

References 4 publications

Genetic diversity of Crotalaria germplasm assessed through phylogenetic analysis of EST-SSR markers

Genetic diversity of Crotalaria germplasm assessed through phylogenetic analysis of EST-SSR markers

Functional Properties of Protein Concentrates from Three Chinese Indigenous Legume Seeds

Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics

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