Seed protein electrophoresis in taxonomic and evolutionary studies

Ladizinsky, G.; Hymowitz, T.

doi:10.1007/bf00263044

Cited by 180 publications

(78 citation statements)

References 64 publications

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“…Total seed proteins electrophoresis has been considered valid for addressing taxonomic studies (Ladizinsky and Hymowitz, 1979), and some of them have determined genetic variability in several legumes (Jha and Ohri, 2002;Vaz et al, 2004;Emre et al, 2006). Interestingly, L. mariae-josephi exhibited marked different protein pattern when it was compared with Old World and New World lupins (Figure 2).…”

Section: Protein Electrophoresismentioning

confidence: 99%

Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)

Múzquiz

Guillamón

Burbano

et al. 1970

Span. j. agric. res.

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A new lupin from Valencia (Spain) has been described recently. The new species Lupinus mariae-josephi H. Pascual (Fabaceae) differs from any other Mediterranean and North American taxon in banner position, flower colour and inflorescence structure. This species was found in a calcareous area, and when it was grown in experimental fields it has always performed better in alkaline soils. This work attempts to establish the content of protein, oil and nutritionally active factors (alkaloids, α-galactosides and inositol phosphates) in this new species of lupin, to investigate differences with other Lupinus ssp. The analysed raw seed samples of this new species showed a total protein content of 329.4 g kg -1 and oil content of 54.0 g kg -1 . The total content of alkaloids, α-galactosides and phytate was similar to other bitter Lupinus species being lupanine, stachyose and myo-inositol hexaphosphate (IP 6 ) the main bioactive compounds. However, the profile of alkaloids and proteins, which is an important chemotaxonomic criterion, was characteristic of this species. It is worth to notice that this Lupinus is able to grow in calcareous soils what makes this species very promising for soil recovery.Additional keywords: alkaloids; fatty acids; lupin; phytate; protein pattern; α-galactosides. ResumenComposición química de una nueva especie de Lupinus encontrada en España: Lupinus mariae-josephi H. Pascual (Fabaceae) Recientemente se ha descrito una nueva especie de Lupinus en Valencia (España). Esta nueva especie, Lupinus mariae-josephi H. Pascual (Fabaceae) difiere de otras especies de Lupinus del área mediterránea o de Norteamérica en la posición del estandarte, el color de la flor y la estructura de la inflorescencia. Fue encontrada en un área calcárea, y en estos últimos años, cuando se ha cultivado, ha crecido en suelos calizos. El objetivo de este trabajo ha sido estudiar el contenido de proteína, grasa y factores nutricionalmente activos (alcaloides, α-galactósidos y fitatos) en esta nueva especie, para ver las diferencias que existen con otras especies de Lupinus. Los resultados han mostrado un contenido en proteína de 329,4 g kg -1 y un contenido en grasa de 54,0 g kg -1 . El contenido total de alcaloides, α-galactósidos y fitatos fue similar a otras especies amargas de Lupinus siendo la lupanina, la estaquiosa y el mioinositol hexafosfato (IP 6 ) los principales componentes bioactivos. Sin embargo, el perfil de alcaloides y el perfil proteico, que son un importante criterio taxonómico, fueron característicos de esta especie. Es importante resaltar que esta especie de Lupinus es capaz de crecer en suelos calizos, lo que la hace muy prometedora para la recuperación de suelos.

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Section: Protein Electrophoresismentioning

confidence: 99%

Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)

Múzquiz

Guillamón

Burbano

et al. 1970

Span. j. agric. res.

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“…Electrophoresis of proteins has been successfully used for the characterization of different taxonomic, evolutionary and genetic relationship studies [23][24][25].…”

Section: Sds Protein Profilementioning

confidence: 99%

Potential Plant Growth-Promoting Activity of Pseudomonas sp Isolated from Paddy Soil in Malaysia as Biocontrol Agent

Saini¹,

Sarin²

2012

J Plant Pathol Microbiol

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“…Electrophoretic analysis of seed proteins has proven to be a valuable tool in tracing the evolution of crop plants, especially for identification of the wild progenitors of the respective crops and gathering additional information on the evolutionary and domestication patterns [47,48]. The presumed ancestral forms and evolutionary patterns of chickpea [49], maize [50], wheat [51] and soybean [52,53] have been identified and established by studying the electrophoretic variability of their respective seed proteins.…”

Section: Phaseolin Polypeptides As Evolutionary Markers -French Bean mentioning

confidence: 99%

Phaseolin: Structure and Evolution

Emani¹,

Hall²

2008

TOEVOLJ

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Abstract:Phaseolin is the salt-soluble glycoprotein or the group of polypeptides of the French bean (Phaseolus vulgaris L.) that account for some 50% of the total protein in mature bean seeds. It was one of the first plant proteins to be translated in vitro from mRNA and one of the first plant genes isolated. It was also the first developmentally regulated plant gene to be expressed in a heterologous plant species through Agrobacterium-mediated transformation. Studies on phaseolin have provided insight to many aspects of plant protein synthesis, from fundamental molecular mechanisms to practical goals such as the improvement of the French bean's nutritional quality. The present review is a comprehensive account of the structural and functional features of phaseolin that have implications regarding its evolution. Additionally, future directions in phaseolin evolutionary studies and suggestions regarding effective and safe biotechnological approaches for the nutritional improvement of French bean seed are outlined.

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Seed protein electrophoresis in taxonomic and evolutionary studies

Cited by 180 publications

References 64 publications

Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)

Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)

Potential Plant Growth-Promoting Activity of Pseudomonas sp Isolated from Paddy Soil in Malaysia as Biocontrol Agent

Phaseolin: Structure and Evolution

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