Seed priming with selenium nanoparticle and plant growth promoting rhizobacteria improve seedling development of foxtail millet (Setaria italica) under salinity stress

Abstract

Much research has been carried out in agriculture and physiology to minimize the adverse effects of salinity on crops. This study was conducted to investigate the effect of selenium nanoparticles and plant growth-promoting bacteria (PGPRs) pretreatment on increasing the resistance of foxtail millet to salinity stress. Experimental treatments consisted of selenium nanoparticles at a concentration of 1 mg L-1, Bacillus cereus and Pseudomonas fluorescens and three different levels of saline solution with concentrations of 0, 100 and 200mM sodium chloride. In this study, foxmillet seeds were primming with solutions of selenium nanoparticles, a variety of bacteria and a mixture of both, and then the pretreated seeds were planted in pots and after two weeks were affected by salinity stress. After 10 days of salinity stress, plants were harvested for analysis. The results of measuring the growth, biochemical and physiological parameters of this study showed that soaking millet seeds in a solution of selenium nanoparticles can increase the plant's ability to cope with salinity stress by increasing photosynthetic pigments, compatible solutes, reducing oxidative stress, protecting the cell membrane and reducing sodium uptake, and it has more effects in the presence of PGPRs bacteria (such as Bacillus cereus and Pseudomonas fluorescens). Therefore, it is suggested that these two biostimulators be used together for greater effects. In addition, since most of the positive functions of these bacteria were not affected by salinity stress, so this type of bacteria can be used with selenium in saline soils.

Antifungal activity of green synthesized selenium nanoparticles and their effect on physiological, biochemical, and antioxidant defense system of mango under mango malformation disease

“…SeNPs also protect the chloroplast structure from severe oxidative damage, such as the breakdown of stroma and grana lamellae and speed up chlorophyll biosynthesis by protecting chloroplast enzymes [ 87 ]. Nasibi et al [ 88 ] reported that pre-inoculation of fox tail seeds with SeNPs under stress, significantly increased chlorophyll content compared to plants grown under salinity stress without seed priming. Additionally, it has been communicated that Pseudomonas fluorescens strains ameliorated chlorophyll fluorescence and chlorophyll pigments in sweet corn under water-deficit stress [ 89 ] Also, ALKahtani et al [ 90 ] observed that treatment of sweet pepper plants with PGPRs, increased the chlorophyll fluorescence and chlorophyll pigment.…”

“…The current study supports the findings of prior research [ 36 , 86 , 92 ]. Nasibi et al [ 88 ], reported that the amount of proline and soluble sugar in foxtail millet plants increased under salinity stress. Proline levels in plant tissues have likely increased because of higher production, less usage in protein synthesis, and enhanced protein hydrolysis as well as decreased proline degradation.…”

A Recent Update on the Impact of Nano-Selenium on Plant Growth, Metabolism, and Stress Tolerance

“…In a previous study [ 149 ], Se-NPs (1 mg L −1 ), various bacteria ( Bacillus cereus, and Pseudomonas fluorescens) , and a combination of both were used to prime foxmillet seeds before they were planted in pots and subjected to salinity stress (0, 100, and 200 mM) after two weeks. The results revealed that the ability of a plant to handle salinity stress could be improved by soaking millet seeds in Se-NP solution, improving photosynthetic pigments, lowering oxidative stress, preserving the cell membrane and suitable solutes, and lowering sodium uptake.…”

Evaluation of Selenium Nanoparticles in Inducing Disease Resistance against Spot Blotch Disease and Promoting Growth in Wheat under Biotic Stress

“…The present results agreed with those of Rady et al [ 83 ], who reported that SeNPs promote physiological attributes against Phaseolus vulgaris . Nasibi et al [ 84 ] reported that pre-inoculation of fox tail seeds with SeNPs under stress significantly increased physiological parameters as compared to plants grown under salinity stress without seed priming. The high uptake of water by plants causes an increase in chlorophyll content [ 85 , 86 ].…”