Seed priming with Salicylic Acid (SA) and Hydrogen Peroxide (H2O2) Improve Germination and Seedling Growth of Wheat (Triticum aestivum) under Salt Stress

Abstract

Aim: Salinity is a major barrier to successful crop production. Seed priming and exogenous application of different signaling molecules can efficiently confer salinity tolerance. Wheat is a major cereal crop in the world and salinity drastically reduces the wheat seedling growth and yield. Therefore, the present study was conducted to explore the potentiality of different signaling molecules such as salicylic acid (SA) and H2O2 to alleviate the salinity-induced growth inhibition of wheat.
 Place and Duration of the Study: The study was conducted in the Department of Seed Science and Technology, Bangladesh Agricultural University, from September-October, 2021.
 Methodology: The wheat (cv. BARI-Gom 24) seeds were soaked in normal tap water (hydro-priming), 1 mM SA, 2 mM SA, 0.1 mM H2O2, and 0.15 mM H2O2 solutions for 30 minutes. The untreated seeds were used as control. Eventually, primed seeds were exposed to 150 mM NaCl in Petri dishes during germination. Primed and non-primed seedlings were grown for 15 days under 150 mM NaCl stress condition.
 Results: The result revealed that salt stress significantly reduced germination percentage (GP), germination index (GI), seed vigor index (SVI), shoot and root length. The results also exhibited that photosynthetic pigments, total chlorophyll, carotenoids, lycopene, and beta-carotene contents were significantly reduced by salt stress. Seed priming with SA and H2O2 and hydro-priming promoted the germination percentage, seedling growth (including shoot and root length), SVI, and photosynthetic pigments.
 Conclusion: Pretreatment with 1 mM SA and 0.1 mM H2O2 was observed to be relatively more efficient in conferring salinity tolerance of wheat compared with other treating conditions. Overall, this study suggests that wheat seed priming with SA and H2O2 and hydro-priming can improve salinity tolerance.
 Aim: Salinity is a major barrier to successful crop production. Seed priming and exogenous application of different signaling molecules can efficiently confer salinity tolerance. Wheat is a major cereal crop in the world and salinity drastically reduces the wheat seedling growth and yield. Therefore, the present study was conducted to explore the potentiality of different signaling molecules such as salicylic acid (SA) and H2O2 to alleviate the salinity-induced growth inhibition of wheat.
 Place and Duration of the Study: The study was conducted in the Department of Seed Science and Technology, Bangladesh Agricultural University, from September-October, 2021.
 Methodology: The wheat (cv. BARI-Gom 24) seeds were soaked in normal tap water (hydro-priming), 1 mM SA, 2 mM SA, 0.1 mM H2O2, and 0.15 mM H2O2 solutions for 30 minutes. The untreated seeds were used as control. Eventually, primed seeds were exposed to 150 mM NaCl in Petri dishes during germination. Primed and non-primed seedlings were grown for 15 days under 150 mM NaCl stress condition.
 Results: The result revealed that salt stress significantly reduced germination percentage (GP), germination index (GI), seed vigor index (SVI), shoot and root length. The results also exhibited that photosynthetic pigments, total chlorophyll, carotenoids, lycopene, and beta-carotene contents were significantly reduced by salt stress. Seed priming with SA and H2O2 and hydro-priming promoted the germination percentage, seedling growth (including shoot and root length), SVI, and photosynthetic pigments.
 Conclusion: Pretreatment with 1 mM SA and 0.1 mM H2O2 was observed to be relatively more efficient in conferring salinity tolerance of wheat compared with other treating conditions. Overall, this study suggests that wheat seed priming with SA and H2O2 and hydro-priming can improve salinity tolerance.

Priming Effect on Seed Germination, Seedling Vigor, and Yield of Bread Wheat (Triticum aestivum L.)

“…Germination was enhanced in distilled water and KNO 3 @ 0.1%. The positive in uence of priming wheat seed in distilled water is in agreement with the ndings of (Tania et al 2021) and that of KNO 3 is in line with the report by (Salehzade et al 2009). Seeds primed with KNO 3 showed improved germination performance, early growth, and vigor index of wheat seedlings (Steiner et al 2018).…”

“…However, Salicylic acid @ 0.06% negatively in uenced the germination of wheat seed. Similarly, (Tania et al 2021) reported that wheat seed primed with 2 mM Salicylic acid for 30 minutes performed lower germination compared to the unprimed control under non-saline stress. Mannitol @ 2% resulted in lower and similar germination capacity with unprimed control.…”

Gibberellic Acid Enhances the Germination and Growth of Maize under Salinity Stress

“…One of the main types of stress that significantly reduces seed germination and crop establishment is salt stress. According to the literature, many crops like wheat, faba beans, and rice can acquire salinity resistance through priming and the addition of various signaling molecules [37][38][39]. The priming of maize seeds with GA 3 improved germination and seedling characteristics under salt stress, as we recently observed [40].…”

Effects of Seed Priming with Proline and Glycine Betaine on Germination, Seedling Growth, and Photosynthetic Pigments of Rice (Oryza sativa) under Chilling Stress

“…Seed germination and seedling establishment is very crucial for plant growth and productivity which often interrupted by some adverse conditions called stress like moisture, salinity, temperature, pathogen, weed, etc. Plants are known to be more tolerant when seeds are primed before being exposed to any stress [13][14]. In this study, it was investigated that seed priming could be helpful for faster and higher seed emergence, better survival and growth of rice seedlings when exposed to cold stress.…”