Seed priming with ascorbic acid enhances salt tolerance in micro-tom tomato plants by modifying the antioxidant defense system components

Alves, Rita de Cássia; Rossatto, Davi Rodrigo; Silva, Jeferson dos S. da; Checchio, Mirela Vantini; Oliveira, Kevein Ruas; Oliveira, Francisco de Asevedo Mendes de Rodrigo; Queiroz, Samira Furtado de; Cruz, Mara Cristina Pessôa da; Gratão, Priscila Lupino

doi:10.1016/j.bcab.2021.101927

Cited by 23 publications

(15 citation statements)

References 71 publications

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“…In general, primed seed produced a lower content of MDA, which keeps the cell membrane stable and reduces ROS production by detoxifying them into non-toxic molecules, resulting in reduced oxidative stress and reduced membrane damage [52]. The effectiveness of AA priming agents in reducing ROS levels and the avoiding of lipid peroxidation was confirmed by Alves et al [29]. In the defense of plants against oxidative stress, vitamins with their antioxidant properties play an important role as free radical scavengers.…”

Section: Discussionmentioning

confidence: 92%

“…The most pronounced effect on total germination was achieved with ascorbic acid (AA), while after H 2 O 2 treatment, seeds germinated faster. According to Alves et al [ 29 ], a positive effect of AA is as a result of its influence on numerous processes that improve the physiological activity of embryos and future seedlings. The positive effects of the application of H 2 O 2 as a priming agent on germination dynamics have been confirmed previously [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…The regulatory effect of seed priming is closely related to the activation of the antioxidant protection system, the removal of reactive oxygen species (ROS), or the increased biosynthesis of phenolic compounds [ 3 ]. In addition, MDA content, as a final byproduct of lipid peroxidation, represents an indicative measure of oxidative damage [ 29 ]. In general, primed seed produced a lower content of MDA, which keeps the cell membrane stable and reduces ROS production by detoxifying them into non-toxic molecules, resulting in reduced oxidative stress and reduced membrane damage [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

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Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Kanjevac

Jakovljević

Todorović

et al. 2022

Plants

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Radish (Raphanus sativus L.) is a vegetable cultivated worldwide because of its large succulent hypocotyls. The priming method initiates metabolic processes at early stages and regulates the metabolic events in seed necessary for germination. This research was conducted to examine the influence of various priming treatments on physiological performance (germination, growth, lipid peroxidation, primary and secondary metabolism) and antioxidant activity of radish seedlings. On the basis of germination and growth characteristics, vigor index, and relative water content in leaves, it was confirmed that priming treatments with 0.01% ascorbic acid (AA) and 1% KNO3 improves the initial stages of radish development. Furthermore, the efficiency of AA as a priming agent was confirmed through the reduction of malondialdehyde (MDA) level compared to unprimed seedlings. On the other hand, hormopriming with indole-3-acetic acid (IAA) significantly increased the concentration of photosynthetic pigments and total soluble leaf proteins compared to non-primed seedlings. The highest content of total phenolic compounds, including flavonoids, were obtained after hormopriming with 1 mM IAA and halopriming with 1% MgSO4. On the basis of the percentage of inhibition of DPPH radicals, it was confirmed that treatments with IAA and AA can improve the antioxidant activity of radish seedlings. This study provides useful information regarding the possibilities of pregerminative metabolic modulation through the seed priming for the biochemical and physiological improvement of radish, and this topic should be further investigated in order to determine the potential use of AA and IAA as suitable priming agents in radish commercial production.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Kanjevac

Jakovljević

Todorović

et al. 2022

Plants

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“…Although AsA has been known to improve plant defense antioxidant system against salt enriched conditions, little is known about the physiological and biochemical responses of AsA-mediated alleviation of salt stress in tomatoes. It has been previously reported that AsA is able to reverse the adverse effects of salt stress on plant growth through enhancing stress-responsive proteins [59]; thus, it has been employed as a priming agent to alleviate the deleterious effects of salt in tomatoes [60]. Our results demonstrated that salt tolerant genotypes, such as LA1579, V1 and V6, exposed to salt stress, maintained their leaf AsA content at control levels, while V2 and AC showed a significant decrease (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Utilization of Tomato Landraces to Improve Seedling Performance under Salt Stress

2021

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Salt stress is considered as one of the most frequent factors limiting plant growth and productivity of crops worldwide. The aim of the study was to evaluate physiological and biochemical responses of nine diverse tomato genotypes exposed to salt stress. In this regard, four-week-old seedlings of one modern variety, five landraces, the salt-sensitive accession of ‘Ailsa Craig’, the salt-tolerant wild accession of S. pimpinellifolium ‘LA1579’, as well as the vitamin C-rich S. pennellii introgression line ‘IL12-4’, were exposed to moderate salt stress (200 mM NaCl) for 10 days. At the end of the stress treatment, agronomical traits and stress indices were evaluated, while gas exchange-related parameters, root electrolyte leakage, malondialdehyde content and ascorbic acid were also determined. All parameters were significantly affected by salt stress, but to a different extent, verifying the diverse degree of tolerance within the selected genotypes, and further highlighting the different stress-induced mechanisms. The landrace originated from ‘Santorini’ island, as well as the modern variety, which originated from traditional cultivars, demonstrated a better performance and adaptivity under moderate salt stress, accompanied by reduced lipid peroxidation and enhanced ascorbic acid content, indicating that they could be potential promising genetic material for breeding programs or as grafting rootstocks/scions.

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“…One way to increase resistance is to increase the level of substrates of antioxidant enzymes and intracellular antioxidants such as ascorbic acid (Paciolla et al, 2019). Ascorbic acid is a small water-soluble molecule with high antioxidant properties and acts as a primary substrate in cyclic pathways to detoxify and neutralize single superoxide and oxygen radicals (Alves et al, 2021). External use of ascorbic acid increases resistance to drought stress and reduces the harmful effects of oxidative stress (Khazaei and Estaji, 2020).…”

Section: Introductionmentioning

confidence: 99%

The effect of ascorbic acid and bio fertilizers on basil under drought stress

et al. 2024

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Evaluate the effect of ascorbic acid application and coexistence of Mycorrhiza fungus and Azospirillium on basil (Ocimum basilicum L.) under drought stress. This experiment was performed as a split factorial in a randomized complete block design with three replications in the crop year 2017-2018 in Shahriar, Iran. In this experiment, irrigation was the main factor in three levels, including drought stress based on 40-70-100 mm from the evaporation pan of class A. Biofertilizer including growth-promoting bacteria (Azospirillium) and mycorrhiza fungus in four levels, including a(Non-consumption) B (Seeds of growth-promoting bacteria (Azospirillium)) C (Consumption of mycorrhiza fungus as seeds) D (Concomitant use of growth-promoting bacteria Azospirillium with mycorrhiza fungi as seeds) and ascorbic acid in two levels of foliar application, including A (Absence Application of ascorbic acid) and B (Application of ascorbic acid (two days after irrigation treatment)) was considered as a factorial factor. The results showed that the highest biological yield was obtained in drought stress of 40 mm and application of biological fertilizers in the form of mycorrhiza application with an average of 3307.1 kg/ha, which was about 70% more than 100 mm evaporation stress and no application of biological fertilizer. The use of ascorbic acid under drought stress conditions improved by 10%, the essential oil using ascorbic acid evaporated under drought stress conditions of 100 mm. As a general conclusion, the use of ascorbic acid and Mycorrhiza + Azospirillium biological fertilizer improved the quantitative and qualitative characteristics of basil under drought stress.

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Seed priming with ascorbic acid enhances salt tolerance in micro-tom tomato plants by modifying the antioxidant defense system components

Cited by 23 publications

References 71 publications

Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Utilization of Tomato Landraces to Improve Seedling Performance under Salt Stress

The effect of ascorbic acid and bio fertilizers on basil under drought stress

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