Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Abstract

Radish (Raphanus sativus L.) is a vegetable cultivated worldwide because of its large succulent hypocotyls. The priming method initiates metabolic processes at early stages and regulates the metabolic events in seed necessary for germination. This research was conducted to examine the influence of various priming treatments on physiological performance (germination, growth, lipid peroxidation, primary and secondary metabolism) and antioxidant activity of radish seedlings. On the basis of germination and growth characteristics, vigor index, and relative water content in leaves, it was confirmed that priming treatments with 0.01% ascorbic acid (AA) and 1% KNO3 improves the initial stages of radish development. Furthermore, the efficiency of AA as a priming agent was confirmed through the reduction of malondialdehyde (MDA) level compared to unprimed seedlings. On the other hand, hormopriming with indole-3-acetic acid (IAA) significantly increased the concentration of photosynthetic pigments and total soluble leaf proteins compared to non-primed seedlings. The highest content of total phenolic compounds, including flavonoids, were obtained after hormopriming with 1 mM IAA and halopriming with 1% MgSO4. On the basis of the percentage of inhibition of DPPH radicals, it was confirmed that treatments with IAA and AA can improve the antioxidant activity of radish seedlings. This study provides useful information regarding the possibilities of pregerminative metabolic modulation through the seed priming for the biochemical and physiological improvement of radish, and this topic should be further investigated in order to determine the potential use of AA and IAA as suitable priming agents in radish commercial production.

Exploring the use of chamomile (Matricaria chamomilla L.) bioactive compounds to control flixweed (Descurainia sophia L.) in bread wheat (Triticum aestivum L.): Implication for reducing chemical herbicide pollution

“…The radicle length of at least 2 mm was considered as the germination phase. The germination rate was calculated using the following formula ( Kanjevac et al, 2022 ): G 1 , G 2 , and G n are the number of germinated seeds calculated at the first, second and last counts; N 1 , N 2 , and N n are the number of days after sowing to the first, second, and last counts.…”

Seed biopriming as a promising approach for stress tolerance and enhancement of crop productivity: a review

“…Priming with IAA improved antioxidant activity, total phenolic compounds, and photosynthetic pigments in radish (Raphanus sativus L.). 37 Nutripriming Nutripriming is the priming of seeds in a solution of nutrients to induce the metabolic processes in seeds and fulfil their nutritional requirements. 38 Priming with different macro and micronutrients regulates many biochemical and physiological processes during the initial seed germination stages.…”

“…reported improved germination and reduced electrical conductivity, MDA and proline content in black cumin ( Nigella sativa L.) primed with salicylic acid under cadmium stress. Priming with IAA improved antioxidant activity, total phenolic compounds, and photosynthetic pigments in radish ( Raphanus sativus L.) 37 …”

Effect of Aqueous and Alcoholic Extracts of Melissa Officinalis on Germination, Growth and Yield of Cultivated Red Radish (Raphanus Sativus L.)

“…After hormopriming with 1 mM IAA and halopriming with 1 % MgSO4, total phenolic compounds, including flavonoids, were greatest. Based on DPPH radical inhibition, IAA and AA may boost radish seedlings' antioxidant activity (Kanjevac et al, 2022). Seed germination and early seedling development of 10 summer annual species were tested in Petri dishes and pot trials to investigate how aqueous extracts of the fragrant herb Melissa officinalis affected these processes.…”