Seed Moisture Isotherms, Sorption Models, and Longevity

Hay, Fiona R.; Rezaei, Shabnam; Buitink, Julia

doi:10.3389/fpls.2022.891913

Cited by 15 publications

(24 citation statements)

References 113 publications

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“…In our EPPO treatment, the rice seeds were pre-equilibrated at 20°C and 40% RH, at which moisture level, we assume the seeds are still in a glassy state and metabolically inactive ( Sun, 1997 ; Ballesteros et al., 2020 ; Hay et al., 2022b ). In the EPPO system, aging occurs in closed chambers filled with high-pressure dry air and with silica gel pre-equilibrated at the same RH as the seeds to provide a buffer for any change in the seed moisture level due to filling with dry air ( Bartolotta et al., 1991 ).…”

Section: Discussionmentioning

confidence: 99%

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Kodde

Angenent

et al. 2022

Front. Plant Sci.

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Seed aging during storage results in loss of vigor and germination ability due to the accumulation of damage by oxidation reactions. Experimental aging tests, for instance to study genetic variation, aim to mimic natural aging in a shorter timeframe. As the oxidation rate is increased by elevating the temperature, moisture, and oxygen levels, this study aimed to (1) investigate the effect of experimental rice seed aging by an elevated partial pressure of oxygen (EPPO), (2) elucidate the mechanism of dry-EPPO aging and (3) compare aging under dry-EPPO conditions to aging under traditional moist-controlled deterioration (CD) conditions and to long-term ambient storage. Dry seeds from 20 diverse rice accessions were experimentally aged under EPPO (200 times higher oxygen levels), at 50% relative humidity (RH), along with storage under high-pressure nitrogen gas and ambient conditions as controls. While no decline in germination was observed with ambient storage, there was significant aging of the rice seeds under EPPO storage, with considerable variation in the aging rate among the accessions, with an average decline toward 50% survival obtained after around 21 days in EPPO storage and total loss of germination after 56 days. Storage under high-pressure nitrogen gas resulted in a small but significant decline, by an average of 5% germination after 56 days. In a second experiment, seven rice seed lots were stored under EPPO as compared to a moist-CD test and two different long-term ambient storage conditions, i.e., conditioned warehouse seed storage (CWSS) and traditional rice seed storage (TRSS). Untargeted metabolomics (with identification of lipid and volatile compounds profiles) showed a relatively high increase in levels of oxidized lipids and related volatiles under all four storage conditions. These compounds had a high negative correlation with seed viability, indicating oxidation as a main deteriorating process during seed aging. Correlation analysis indicated that EPPO storage at 50% RH is more related to aging under TRSS at 60% and CD-aging at 75% ERH rather than CWSS at 40% ERH. In conclusion, aging rice seeds under EPPO conditions is a suitable experimental aging method for analyzing variation among seed lots or genotypes for longevity under storage.

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Section: Discussionmentioning

confidence: 99%

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Kodde

Angenent

et al. 2022

Front. Plant Sci.

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“…Delimiting the boundaries of this oxidative window is complicated by the external factors of dry storage (or after-rippening), especially the temperature and the relative humidity which can both influence seed cytoplasmic viscosity. As a result, these two factors can affect seed metabolism and physiology, potentially leading to reduced dormancy alleviation, seed aging and viability loss [29][30][31] . Despite these insights, a considerable knowledge gap exists regarding how changes in cytoplasmic viscosity impact dormancy release, oxygen diffusion and subsequent non-enzymatic ROS production via autoxidation reactions (within dehydrated seed tissues).…”

Section: Introductionmentioning

confidence: 99%

Release of Arabidopsis seed dormancy by cold atmospheric plasma relies on cytoplasmic glass transition

August

Dufour

Bailly³

2023

J. Phys. D: Appl. Phys.

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When mature Arabidopsis thaliana seeds are dormant, their germination is prevented in apparently favourable conditions. This primary dormancy can be released during seed dry storage through a process called after-ripening whose duration can last several months. To reduce this delay, cold atmospheric plasmas (CAP) can be used as sources of reactive oxygen species capable of inducing heterogeneous chemical reactions. While CAP are known to stimulate the germination of various seed species, the relationship between CAP treatments and the amorphous solid state of dry seeds remains unexplored. Here, we demonstrate that seed dormancy can be alleviated using a cold plasma of ambient air and that this alleviation can be amplified for seeds with high water-content (typically 30 %DW) or seeds heated at 60 °C during plasma treatment. Differential scanning micro-calorimetry shows that these characteristics control the glassy/rubbery state of the seed cytoplasm. This technique indicates also that a glass transition to the rubbery state strengthens the CAP effects to alleviate seed dormancy. We propose that lower cytoplasmic viscosity can promote the oxidative signaling induced by CAP which, in turn, improves the germination process.

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“…Lipid peroxidation and oxidation of biomolecules have been considered as the major reason of seed deterioration during the aging process [ 5 ]. Seed with higher moisture had a higher water activity that accelerated the rate of lipid peroxidation and Maillard reaction [ 30 , 31 ] thus seed germination is reduced. In hermetic system, seed MC must be lower (2–3%) than that at which seeds are normally packed in non sealed storage.…”

Section: Discussionmentioning

confidence: 99%

Hermetic storage of okra seed maintains seed longevity under changing environment

et al. 2023

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Okra seed is vulnerable to loss of germination and vigor in variable storage conditions. High seed moisture contents (SMC) accelerate seed deterioration during storage thus keeping low seed moisture contents by storing seed in hermetic bags may help to retain seed longevity. Okra seed was equilibrated to four initial moisture levels including 8,10, 12 and 14% SMC. Seed was then packed and stored in traditional storage bags (Paper, cloth, polypropylene and jute bag) and hermetic Super Bag for 12 months under ambient conditions. Seed stored in hermetic Super Bag at 8 and 10% moisture contents maintained higher germination due to low seed moisture contents. Moreover, activities of α-amylases and total soluble sugars were higher while electrical conductivity of seed leachates, malondialdehyde (MDA) and reducing sugar contents were less in the seeds stored in hermetic Super Bag at 8 and 10% SMC as compared to seed stored in traditional storage bags. Hermetic storage at 14% moisture negatively influenced the seed quality. Moisture adsorption isotherms of okra seeds were developed at constant temperature of 25°C and varying levels of relative humidity from 60 to 90%. Moisture isotherms indicated no significant increase in seed moisture contents at 60 and 70% relative humidity (RH) in hermetic bags whereas a minor increase in seed moisture at 80 and 90% RH has been observed for the seeds incubated in hermetic bags. SMC significantly increased in traditional storage bags particularly in jute bag at high RH. In conclusion, storage in hermetic bags, maintain low seed moisture and high seed quality. Okra seed storage in hermetic bags at 8 and 10% SMC maintains seed longevity under ambient storage conditions.

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Seed Moisture Isotherms, Sorption Models, and Longevity

Cited by 15 publications

References 113 publications

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

Release of Arabidopsis seed dormancy by cold atmospheric plasma relies on cytoplasmic glass transition

Hermetic storage of okra seed maintains seed longevity under changing environment

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