Seed germination and seedling growth promoted by a Ceratobasidiaceae clone in Vanda thwaitesii Hook. f., an endangered orchid species endemic to South Western Ghats, India and Sri Lanka

Decruse, S. William; Neethu, RS; Pradeep, N. S.

doi:10.1016/j.sajb.2018.04.002

Cited by 15 publications

(20 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Orchids associating with a limited number of fungi or with fungi that have a restricted distribution (Waud et al, 2016) can be expected to have narrower distribution patterns compared to orchids that associate with a large number of fungi or with fungi that have a broad distribution (Davis et al, 2015;Těšitelová et al, 2015). Better insights into the relationships between orchid seeds and associated fungi may therefore improve our understanding of the various factors that determine the geographic distribution of orchid species and at the same time benefit symbiotic seedling propagation for in situ and ex situ conservation and reintroduction programs (Fracchia et al, 2014(Fracchia et al, , 2016Shao et al, 2017;Decruse et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Co-Cultures of Mycorrhizal Fungi Do Not Increase Germination and Seedling Development in the Epiphytic Orchid Dendrobium nobile

2020

View full text Add to dashboard Cite

Orchids are highly dependent on mycorrhizal fungi for seed germination and subsequent growth to a seedling as they provide essential carbon, water, and mineral nutrients to developing seeds. Although there is mounting evidence that orchid seeds are often colonized by multiple fungi simultaneously, most in vitro germination experiments focus on mycorrhizal monocultures and little is known about how mycorrhizal assemblages affect seed germination and growth of seedlings. In this study, we compared the effects of mycorrhizal monocultures and co-cultures on seed germination and seedling growth of the epiphytic orchid Dendrobium nobile. In situ baiting was used to isolate mycorrhizal fungi from protocorms for germination experiments. Germination experiments were conducted under two light regimes for 90 days. In total, five fungal strains were isolated from protocorms of D. nobile, indicating that the species was not highly specific to its fungal partners. Four strains (JC-01, JC-02, JC-04, and JC-05) belonged to the Serendipitaceae and one (JC-03) to the Tulasnellaceae. In vitro germination experiments showed that germination percentages were higher under light-dark conditions than under complete dark conditions, supporting previous findings that light facilitates germination in epiphytic orchids. While all strains were able to induce protocorm formation and growth into the seedling stage, large differences between fungal strains were observed. Co-cultures did not result in significantly higher seed germination percentages and seedling development than monocultures. Taken together, these results demonstrate that effects of fungal assemblages are not predictable from those of component species, and that more work is needed to better understand the role of fungal assemblages determining seed germination and subsequent growth under natural conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

Co-Cultures of Mycorrhizal Fungi Do Not Increase Germination and Seedling Development in the Epiphytic Orchid Dendrobium nobile

2020

View full text Add to dashboard Cite

show abstract

“…[18]. Endophytic fungi can deliver appropriate carbon sources, amino acids, vitamins, and hormones that are important for seedling and protocorm development [19]. Khamchatra et al [3] also stated that Beauvaria and Fusarium species are endophytic fungi, which we also recovered, that may play a role in the growth and survival of the plants like D. friedericksianum.…”

Section: Discussionmentioning

confidence: 56%

“…The symbiotic plant relationship, specifically in vitro approaches, is adopted because it enables higher growth rates and/or symbiotic seedlings and protocorms progress faster than without this relationship [11]. Although extensive information about the co-culturing expansion is not available, the process was endorsed as an effective process for improving the growth of many orchids [19].…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Endophytic Fungi and Their Pathogenicity Evaluation Against Dendrobium nobile and Dendrobium officinale

Sarsaiya

Jain

Fan

et al. 2020

IJMS

View full text Add to dashboard Cite

Dendrobium are tropical orchid plants that host diverse endophytic fungi. The role of these fungi is not currently well understood in Dendrobium plants. We morphologically and molecularly identified these fungal endophytes, and created an efficient system for evaluating the pathogenicity and symptoms of endophytic fungi on Dendrobium nobile and Dendrobium officinale though in vitro co-culturing. ReThe colony morphological traits of Dendrobium myco-endophytes (DMEs) were recorded for their identification. Molecular identification revealed the presence of Colletotrichum tropicicola, Fusarium keratoplasticum, Fusarium oxysporum, Fusarium solani, and Trichoderma longibrachiatum. The pathogenicity results revealed that T. longibrachiatum produced the least pathogenic effects against D. nobile protocorms. In seedlings, T. longibrachiatum showed the least pathogenic effects against D. officinale seedlings after seven days. C. tropicicola produced highly pathogenic effects against both Dendrobium seedlings. The results of histological examination of infected tissues revealed that F. keratoplasticum and T. longibrachiatum fulfill Koch’s postulates for the existence of endophytes inside the living tissues. The DMEs are cross-transmitted inside the host plant cells, playing an important role in plant host development, resistance, and alkaloids stimulation.

show abstract

“…Though uncommon, studies showing non- Rhizoctonia -like fungi forming OM have increased since the early 2000s [ 20 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Those genera include strong pathogen [ 6 , 30 , 33 , 38 ], saprobes [ 39 , 40 ], ectomycorrhizal fungi [ 41 , 42 ], and ascomycetous fungi [ 20 , 43 ]. Another unique orchid trait is mycoheterotrophy, which involves the utilization of OM symbiosis to obtain carbon, but does not reciprocally provide the fungi any nutrients [ 42 , 44 ].…”

Section: Through the Looking Glass: How Orchids Naturally Establismentioning

confidence: 99%

“…Since in situ studies are complex and impractical [ 67 ], in vitro symbiotic germination studies will contribute to understanding the interaction between orchids and OMF in nature. Several studies also emphasized the importance of mycorrhizal interaction to plant quality during acclimatization, e.g., increase of leaf size, accelerated flowering time, and survival rate [ 10 , 13 , 39 , 90 ]. These parameters are important for a plant growing in its natural habitat due to dynamic conditions [ 91 ].…”

Section: Future Perspectivementioning

confidence: 99%

In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

Pujasatria

Miura

Kaminaka

2020

Plants

View full text Add to dashboard Cite

As one of the largest families of flowering plants, Orchidaceae is well-known for its high diversity and complex life cycles. Interestingly, such exquisite plants originate from minute seeds, going through challenges to germinate and establish in nature. Alternatively, orchid utilization as an economically important plant gradually decreases its natural population, therefore, driving the need for conservation. As with any conservation attempts, broad knowledge is required, including the species’ interaction with other organisms. All orchids establish mycorrhizal symbiosis with certain lineages of fungi to germinate naturally. Since the whole in situ study is considerably complex, in vitro symbiotic germination study is a promising alternative. It serves as a tool for extensive studies at morphophysiological and molecular levels. In addition, it provides insights before reintroduction into its natural habitat. Here we reviewed how mycorrhiza contributes to orchid lifecycles, methods to conduct in vitro study, and how it can be utilized for conservation needs.

show abstract

Seed germination and seedling growth promoted by a Ceratobasidiaceae clone in Vanda thwaitesii Hook. f., an endangered orchid species endemic to South Western Ghats, India and Sri Lanka

Cited by 15 publications

References 40 publications

Co-Cultures of Mycorrhizal Fungi Do Not Increase Germination and Seedling Development in the Epiphytic Orchid Dendrobium nobile

Co-Cultures of Mycorrhizal Fungi Do Not Increase Germination and Seedling Development in the Epiphytic Orchid Dendrobium nobile

Molecular Identification of Endophytic Fungi and Their Pathogenicity Evaluation Against Dendrobium nobile and Dendrobium officinale

In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

Contact Info

Product

Resources

About