Seed biopriming with indigenous endophytic bacteria isolated from Wakatobi rocky soil to promote the growth of onion (Allium ascalonicum L.)

Abstract

The realization of sustainable agriculture requires continuous research which can reduce the use of chemical fertilizers. This research was carry out to obtain the potential indigenous endophytic bacteria isolated from onion planted on rock soil of Wakatobi district in Southeast Sulawesi. The study conducted using a completely randomized design (CRD) consisting of 9 isolates. Germination was recorded daily until 14 days. At the same time, isolates evaluated for their ability to solubilize phosphate, fix N and synthesize IAA. Results showed that the seed treatments using endophytic bacteria significantly increased viability onion seeds, in term of germination increases in germination percentage. Almost all endophytic bacteria isolate tested were able to solubilize phosphate, fix N and synthesize IAA. From this study, Ke03 isolates showed the best performance and were able to increase germination percentage, root length and number of onion roots reaching 145%, 46% and 78% respectively, as compared to controls. The ability of Ke03 isolates to increase onion germination correlated with the ability of this isolate to synthesize IAA (80.33 ppm). Further research is needed to evaluate whether this isolate is effective in increasing the growth and yield of onions in the field.

Seed Biopriming Using Rhizobacterial Isolated Mixture on Increasing Growth and Yield of Shallots (Allium ascalonicum L.)

“…Previous research results indicated that the three rhizobacterial isolates were proven to be able to stimulate plant growth [17], [18]. The role of rhizobacteria as plant growth promoters is related to their ability to dissolve phosphate, fix N and produce growth hormone IAA [19], [5], [20].…”

“…group. and Pseudomonas fluorescens, [5]. This type of bacteria has advantages in addition to promote plant growth, it is also able to protect plants from various plant disease infections.…”

Wakatobi Native Bacteria Inhibit The Colony Growth of Alternaria porri and Fusarium oxyporum of Shallots in in Vitro Study

“…Para petaninya membudidayakan bawang merah dengan cara konvensional, tanpa pupuk kimia dan pestisida sehingga kondisi tanahnya masih alami. Mikroba indigenos (bakteri endofit) yang diisolasi dari tanaman bawang merah di Wakatobi terbukti efektif meningkatkan viabilitas dan vigor benih bawang merah (Sutariati et al 2019). Bakteri endofit sebagai agens biokontrol memiliki kelebihan karena mempunyai kemampuan bertahan terhadap tekanan biotik dan abiotik (Resti et al 2013).…”

Efektivitas Rizobakteri Sebagai PGPR Untuk Pertumbuhan Stek Daun Tanaman Hias Peperomia Turboensis

“…Hasil penelitian Suatariati et al (2018) melaporkan bahwa rizobakteri mampu berperan sebagai pemacu petumbuhan tanaman. Penggunaan rizobakteri telah terbukti efektif meningkatkan perkecambahan (Mudi et al, 2019;Sutariati et al, 2020), pertumbuhan, dan hasil tanaman kedelai (Suarni et al, 2016;Sutariati et al, 2019).…”