Sediment toxicity assessment using zebrafish (Danio rerio) as a model system: Historical review, research gaps and trends

Saiki, Patrícia Thieme Onofri; Mello-Andrade, Francyelli; Gomes, Tânia; Rocha, Thiago Lopes

doi:10.1016/j.scitotenv.2021.148633

Cited by 20 publications

(6 citation statements)

References 99 publications

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“…AChE plays an important role in nerve impulse conduction and is a recognized biomarker of neurotoxicity in toxicology studies (Saiki et al 2021). The enzyme immediately terminates the continuous excitatory effect of neurotransmitters by hydrolyzing acetylcholine, ensuring the normal transmission of nerve signals in the body (Tao et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress, neurotoxicity, and intestinal microbial regulation after a chronic zinc exposure: an experimental study on adult zebrafish (Danio rerio)

Yuan

et al. 2023

Journal of Water Reuse and Desalination

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Zinc is one of the heavy metals present in textile wastewater with high concentrations. However, the chronic toxic effects of zinc on aquatic vertebrates are still ambiguous. Zinc accumulation in zebrafish after chronic zinc exposure and toxic effects on the intestines, muscles, and gills were investigated in this study. The results showed that a significant accumulation of zinc in the intestine, muscle, and gill was observed after 25 d of zinc exposure. The toxic effects of zinc were mainly in the form of zinc-induced oxidative stress in zebrafish, potential neurotoxicity, and changes in intestinal microbes. Significant changes in the levels of superoxide dismutase, catalase, metallothionein, glutathione, and malondialdehyde indicated that zinc damaged the antioxidant system of adult zebrafish. Zinc exposure resulted in a significant decrease in acetylcholinesterase activity and abnormal neural signaling. Furthermore, zinc exposure resulted in increased intestinal microbial richness and decreased the Simpson index in adult zebrafish. At the phylum and genus levels, the predominant microbes in the intestine are altered by zinc. In summary, this study provides an analysis of the toxic effects of chronic zinc exposure on adult zebrafish and the potential mechanisms, which are important for assessing the dual effects of zinc on aquatic organisms.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress, neurotoxicity, and intestinal microbial regulation after a chronic zinc exposure: an experimental study on adult zebrafish (Danio rerio)

Yuan

et al. 2023

Journal of Water Reuse and Desalination

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“…In recent years, zebrafish ( Danio rerio ) have emerged as an ideal model for disease studies and drug screening due to the advantages such as rapid life cycle, low cost and transparency [ 24 ]. Zebrafish present a fully innate immune system with macrophages and neutrophils for host defense and zebrafish infection models have been established for numerous pathogens [ 25 ]. Our previous study showed that microinjection of LPS into the zebrafish yolk, CuSO 4 and tail fin injury can be used to induce the zebrafish acute inflammation [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Liquiritin exhibits anti-acute lung injury activities through suppressing the JNK/Nur77/c-Jun pathway

Zhou

Yang

et al. 2023

Chin Med

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Background Licorice (Glycyrrhiza uralensis Fisch.), a well-known traditional medicine, is traditionally used for the treatment of respiratory disorders, such as cough, sore throat, asthma and bronchitis. We aim to investigate the effects of liquiritin (LQ), the main bioactive compound in licorice against acute lung injury (ALI) and explore the potential mechanism. Methods Lipopolysaccharide (LPS) was used to induce inflammation in RAW264.7 cells and zebrafish. Intratracheal instillation of 3 mg/kg of LPS was used for induction an ALI mice model. The concentrations of IL-6 and TNF-α were tested using the enzyme linked immunosorbent assay. Western blot analysis was used to detect the expression of JNK/Nur77/c-Jun related proteins. Protein levels in bronchoalveolar lavage fluid (BALF) was measured by BCA protein assay. The effect of JNK on Nur77 transcriptional activity was determined by luciferase reporter assay, while electrophoretic mobility shift assay was used to examine the c-Jun DNA binding activity. Results LQ has significant anti-inflammatory effects in zebrafish and RAW264.7 cells. LQ inhibited the expression levels of p-JNK (Thr183/Tyr185), p-Nur77 (Ser351) and p-c-Jun (Ser63), while elevated the Nur77 expression level. Inhibition of JNK by a specific inhibitor or small interfering RNA enhanced the regulatory effect of LQ on Nur77/c-Jun, while JNK agonist abrogated LQ-mediated effects. Moreover, Nur77-luciferase reporter activity was suppressed after JNK overexpression. The effects of LQ on the expression level of c-Jun and the binding activity of c-Jun with DNA were attenuated after Nur77 siRNA treatment. LQ significantly ameliorated LPS-induced ALI with the reduction of lung water content and BALF protein content, the downregulation of TNF-α and IL-6 levels in lung BALF and the suppression of JNK/Nur77/c-Jun signaling, which can be reversed by a specific JNK agonist. Conclusion Our results indicated that LQ exerts significant protective effects against LPS-induced inflammation both in vivo and in vitro via suppressing the activation of JNK, and consequently inhibiting the Nur77/c-Jun signaling pathway. Our study suggests that LQ may be a potential therapeutic candidate for ALI and inflammatory disorders.

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“…Of the 225 articles published until January 2023, 92 were thoroughly reviewed. Each article used in the study was compiled according to the following parameters: (i) publication year; (ii) the type of pollutant; (iii) exposure factors; (iv) toxicity; (v) stage of development; (vi) types of tests; and (vii) ecotoxic effects (Figure 1) [1,11,12]. manufacture in the laboratory; (iv) prone to genetic changes; and (v) if possible, have relatively small and stable genome [7].…”

Section: Methodsmentioning

confidence: 99%

Danio rerio: A Sustainable Model for Monitoring Pollutants in Aquatic Environments

et al. 2023

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Sediment toxicity assessment using zebrafish (Danio rerio) as a model system: Historical review, research gaps and trends

Cited by 20 publications

References 99 publications

Oxidative stress, neurotoxicity, and intestinal microbial regulation after a chronic zinc exposure: an experimental study on adult zebrafish (Danio rerio)

Oxidative stress, neurotoxicity, and intestinal microbial regulation after a chronic zinc exposure: an experimental study on adult zebrafish (Danio rerio)

Liquiritin exhibits anti-acute lung injury activities through suppressing the JNK/Nur77/c-Jun pathway

Danio rerio: A Sustainable Model for Monitoring Pollutants in Aquatic Environments

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