Sedative and hypnotic effects of compound Anshen essential oil inhalation for insomnia

Abstract

BackgroundsThe chemical composition of many essential oils indicates that they have sedative and hypnotic effects, but there is still a lack of systematic studies on the sedative and hypnotic effects of essential oils. In addition, aromatherapy does not seem to have the side effects of many traditional psychotropic substances, which is clearly worthwhile for further clinical and scientific research. The clinical application of essential oils in aromatherapy has received increasing attention, and detailed studies on the pharmacological activities of inhaled essential oils are increasingly needed.Hypothesis/purposeAs insomniacs are usually accompanied by symptoms of depression and anxiety of varying degrees, based on the theory of aromatherapy of Traditional Chinese Medicine, this experiment is to study a Compound Anshen essential oil that is compatible with Lavender essential oil, Sweet Orange essential oil, Sandalwood essential oil and other aromatic medicine essential oils with sedative and hypnotic effects, anti-anxiety and anti-depression effects. To study the sedative and hypnotic effects of Compound Anshen essential oil inhaled and the main chemical components of Compound Anshen essential oil, and to compare and analyze the pharmacodynamics of diazepam, a commonly used drug for insomnia.MethodsThe Open field test and Pentobarbital-induced sleep latency and sleep time experiments were used to analyze and compare the sedative and hypnotic effects of inhaling Compound Anshen essential oil and the administration of diazepam on mice. The changes of 5-HT and GABA in mouse brain were analyzed by Elisa. The main volatile constituents of Compound Anshen essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS).ResultsInhalation of Compound Anshen essential oil can significantly reduce the spontaneous activity of mice, reduce latency of sleeping time and prolong duration of sleeping time. The results of enzyme-linked immunosorbent assay showed that Compound Anshen essential oil can increase the content of 5-HT and GABA in mouse brain. The main volatile chemical constituents of the Compound Anshen essential oil are D-limonene (24.07%), Linalool (21.98%), Linalyl acetate (15.37%), α-Pinene (5.39%), and α-Santalol (4.8%).ConclusionThe study found that the inhalation of Compound Anshen essential oil has sedative and hypnotic effect. This study provides a theoretical basis for further research and development of the sedative and hypnotic effects of Compound Anshen essential oil based on the theory of aromatherapy.

Therapeutic Effect and Mechanisms of Essential Oils in Mood Disorders: Interaction between the Nervous and Respiratory Systems

“…The decrease of symptoms was associated with an increase of plasma 5-HT in the EO-treated group [ 81 ]. In another study, which used compound anshen EO, the inhalation of the EO improved sleep quality and promoted 5-HT increase in experimental mice [ 82 ]. An increase of 5-HT-expressing neurons in the dorsal raphe nucleus was found in animals that inhaled EO from asarum heterotropoides for 3 h [ 45 ], suggesting that the positive effect of EO inhalation may occur in both acute and chronic conditions.…”

Meme Kanseri ve Aromaterapi

“…25 Yapılan çalışmalarda kokuların nörofizyolojik fonksiyonları etkileyip fiziksel ve ruhsal durumu değiştirebileceği ifade edilmiştir. 30,31 Aromaterapi gibi tamamlayıcı tedavi uygulamaları, kanser hastalarının yaşam kalitesini artırmak amacıyla destekleyici tedaviler olarak ortaya çıkmıştır. Son zamanlarda tamamlayıcı tedavilerin kanser tedavilerinde etkili olarak kullanılması uygulamaların popüler olmasını sağlamıştır.…”

Response of Orchid Cut Flowers as Affected by Floral Preservatives on the Postharvest Quality

“…Limonene inhalation has shown antidepressant activity in mice model with chronic unpredictable mild stress . Similarly, inhalation of Anshen oil which consists of 24.07% D-limonene has decreased the sleep onset latency time and increased the duration of sleeping time in mice model of insomnia (Zhong et al, 2019). R-limonene has also shown antidepressant activity in mice model with spared nerve injury (Piccinelli et al, 2015).…”