Secretory virulence factors produced by Staphylococcus aureus isolates obtained from mastitic bovine milk – effect on bovine polymorphonuclear neutrophils

Fijałkowski, Karol; Czernomysy-Furowicz, D.; Irwin, Jane A.; Nawrotek, Paweł; Pobucewicz, A.

doi:10.1016/j.rvsc.2011.08.017

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…For example, surfactant protein A binds to the Fc fragment of immunoglobulin G making S. aureus "invisible" to phagocytic cells. S. aureus also produces toxins that inhibit neutrophil phagocytic activity (30). In the present study the most encouraging results were found in the group receiving both cefuroxime and the herd-specific autovaccine, in which, between days 7 and 35, all animals were free of S. aureus.…”

Section: Discussionsupporting

confidence: 61%

Herd-specific autovaccine and antibiotic treatment in elimination of Staphylococcus aureus mastitis in dairy cattle

Czernomysy-Furowicz¹,

Fijałkowski²,

Silecka³

et al. 2014

Turk J Vet Anim Sci

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The aim of the present study was to compare the effectiveness of cefuroxime, herd-specific autovaccine, and cefuroxime/herdspecific autovaccine treatments in eliminating S. aureus from the milk of 45 cows with subclinical mastitis. Prior to this study, cows with S. aureus mastitis were divided into 3 groups of 15 cows each. Group 1 received cefuroxime, group 2 received the autovaccine, and group 3 received cefuroxime and the autovaccine. The antibiotic was infused into the teat canal, whereas the autovaccine was administrated in the upper udder lymph node region. It was revealed that after day 35 of the treatment S. aureus was not detected in the milk of 40% of the cows treated with antibiotic, 60% of the cows treated with the autovaccine, and 100% of the cows that underwent combined therapy. Further observation of cows selected for the current study showed that in cows treated with both cefuroxime and a herd-specific autovaccine S. aureus mastitis was not recorded for at least 2 years. In conclusion, this investigation revealed that the administration of an anti-S. aureus herd-specific autovaccine into the upper udder lymph node region combined with infusion of an appropriate antibiotic into the teat canal eliminates S. aureus and leads to prolonged udder protection against invasion by these microorganisms.

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Section: Discussionsupporting

confidence: 61%

Herd-specific autovaccine and antibiotic treatment in elimination of Staphylococcus aureus mastitis in dairy cattle

Czernomysy-Furowicz¹,

Fijałkowski²,

Silecka³

et al. 2014

Turk J Vet Anim Sci

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“…Staphylococcus auerus is the prime etiological agent causing mastitis in bovines (Kalorey et al 2007;Kuang et al 2009). Besides the production of exotoxins and surface proteins (Oliveira et al 2011a;Fijalkowski et al 2011), the formation of highly organized multicellular complexes by bacteria, the biofilms, is increasingly being recognized as an important virulence factor in S. aureus (Oliveira et al 2006;Coelho et al 2011). Biofilm facilitates the adherence and colonization of S. auerus on the mammary gland epithelium, also contributing to the evasion of the immunological defense and to the difficulty of pathogen eradication (Melchior et al 2007;Wu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Chavhan

Nagdive

Purohit

et al. 2011

Trop Anim Health Prod

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Biofilm formation in Staphylococcus aureus is considered an important virulence factor in bovine mastitis. Intercellular adhesion gene A (icaA) is a significant genetic determinant that contributes in biofilm formation. The aim of the present study was to determine the presence of the icaA gene in S. aureus isolated from bovine mastitis from seven states of India. A total of 88 out of 150 Staphylococcus aureus strains were found to be positive for biofilm marker icaA gene by PCR. The icaA gene was confirmed by dot blot hybridization in 41 of 150 S. aureus strains tested. Results obtained with dot blot hybridization were comparable to those obtained with PCR. Partial sequences of the icaA gene of the two S. aureus isolates showed deletion of some bases in different positions that might reduce/stop transcription leading to no biofilm formation. PCR was found to be a rapid test but dot blot hybridization was more accurate than PCR for detection of icaA genes. This study showed that detection of biofilm marker the icaA gene in S. aureus would allow the detection of virulence factors present in mastitis and early application of corrective measures.

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“…Other virulence factors could be associated with the intensive inflammatory response, such as enterotoxins [41]. Fijalkowski et al [42] [43] observed that exogenic virulence factors secreted by S. aureus isolates significantly influenced the digestion efficiency and phagocytosis carried out by bovine polymorphonuclear neutrophils in vitro and cytokine gene expression and cytokine secretion. In the present study, the relationship between the presence of these genes and increased production of cytokines was not observed.…”

Section: Discussionmentioning

confidence: 99%

Virulence Factor Profile of <i>Staphylococcus aureus</i> Isolated from Bovine Milk from Brazil

Santos¹,

Martins²,

Rezende³

et al. 2014

FNS

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This study investigates the biofilm formation, presence and distribution of virulence genes and the capacity to induce an inflammatory response in strains of Staphylococcus aureus isolated from milk samples in Bahia, Brazil. A total of 132 samples of raw milk were collected from four dairy farms (designated A to D) located in southwestern Bahia, in the municipality of Vitória da Conquista, from October/2009 to September/2010. After processing of the samples, 94 (71.2%) S. aureus isolates were obtained. These strains were subjected to the antibiogram method MIC (Minimum Inhibitory Concentration). As for the pathogenicity, tests were performed in vitro biofilm formation induced by glucose. Moreover, we performed PCR for their virulence genes: sea (enterotoxin A), seb (B), sec (C), pvl (Panton-Valentine Leukocidin), clfA (Clumping Factor A) and spa (protein A) and analysis of cytokine induction in the inflammatory response of J774 macrophages by exocellular lipoteichoic acid. No isolates were resistant to oxacillin and vancomycin. In biofilm production, 5.31% (5/94) isolates did not produce biofilm, 5.31% (5/94) of the samples were poor producers, 15.96% (15/94) strains were moderate producers, 18.09% (17/94) were producers and 55.32% (55/94) of isolates were strong biofilm producers. One (1.06%) isolate expressed the seb gene, one (1.06%) sec, 18 (19.2%) cflA and 44 (46.8%) had spa. There was no expression of sea and pvl between isolates analyzed. The analysis of cytokine induction in the inflammatory response did not show statistical difference in the levels of IL-6, TNF-α and IL-10 induction. However, there was statistical difference in IL-1 induction between isolates from different farms. Thus, it

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Secretory virulence factors produced by Staphylococcus aureus isolates obtained from mastitic bovine milk – effect on bovine polymorphonuclear neutrophils

Cited by 6 publications

References 39 publications

Herd-specific autovaccine and antibiotic treatment in elimination of Staphylococcus aureus mastitis in dairy cattle

Herd-specific autovaccine and antibiotic treatment in elimination of Staphylococcus aureus mastitis in dairy cattle

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Virulence Factor Profile of <i>Staphylococcus aureus</i> Isolated from Bovine Milk from Brazil

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Secretory virulence factors produced by Staphylococcus aureus isolates obtained from mastitic bovine milk – effect on bovine polymorphonuclear neutrophils

Cited by 6 publications

References 39 publications

Herd-specific autovaccine and antibiotic treatment in elimination of Staphylococcus aureus mastitis in dairy cattle

Herd-specific autovaccine and antibiotic treatment in elimination of Staphylococcus aureus mastitis in dairy cattle

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Virulence Factor Profile of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; Isolated from Bovine Milk from Brazil

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Virulence Factor Profile of <i>Staphylococcus aureus</i> Isolated from Bovine Milk from Brazil