The aim of the present study was to compare the effectiveness of cefuroxime, herd-specific autovaccine, and cefuroxime/herdspecific autovaccine treatments in eliminating S. aureus from the milk of 45 cows with subclinical mastitis. Prior to this study, cows with S. aureus mastitis were divided into 3 groups of 15 cows each. Group 1 received cefuroxime, group 2 received the autovaccine, and group 3 received cefuroxime and the autovaccine. The antibiotic was infused into the teat canal, whereas the autovaccine was administrated in the upper udder lymph node region. It was revealed that after day 35 of the treatment S. aureus was not detected in the milk of 40% of the cows treated with antibiotic, 60% of the cows treated with the autovaccine, and 100% of the cows that underwent combined therapy. Further observation of cows selected for the current study showed that in cows treated with both cefuroxime and a herd-specific autovaccine S. aureus mastitis was not recorded for at least 2 years. In conclusion, this investigation revealed that the administration of an anti-S. aureus herd-specific autovaccine into the upper udder lymph node region combined with infusion of an appropriate antibiotic into the teat canal eliminates S. aureus and leads to prolonged udder protection against invasion by these microorganisms.
The research was conducted on clinically healthy mares (n=40) and foals (n=78) duringY. pseudotuberculosis associated enzootics. The animals were divided into groups: I to IV -mares, IA to IVA -their offsprings, IB to IVB -foals which mothers were not treated with any medicaments. The animals in group I, IA and IB were injected with PBS; in group II, IIA and IIB -with Y. pseudotuberculosis strain-based vaccine, in group III, IIIA and IIIB -with P. acnes strain-based immunostimulator; in group IV, IVA and IVB -with P. acnes strain-based immunostimulator and (5 days after the immunostimulator injection)Y. pseudotuberculosis strain-based vaccine. The presence of antibodies was determined by means of ELISA. The study revealed anti-Yersinia pseudotuberculosis IgG only in 19 mares before, and in 25 mares and 26 foals 3 weeks after vaccination. The mean extinction 3 weeks after vaccination amounted to: II-0.489, IV-2.578, IIA-0.572, IVA-0.974, IIB-0.312, IVB-0.418. The cut-off extinction value was 0.154. The presence of anti-Yersinia pseudotuberculosis IgG before vaccination in the sera of clinically healthy mares may suggest that Y. pseudotuberculosis infection occurs definitely more often than is expected. Vaccination preceded by immunostimulation appeared to be the most efficient method of treatment against yersiniosis.
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