Secretory response to cholera toxin in the porcine jejunum under different types of general anaesthesia

Maltbaek, JS; Ml, Grøndahl; Berggreen, Paul; Nielsen, CG; Thorboll, JE; Skadhauge, Erik; Mb, Hansen

doi:10.1113/expphysiol.1998.sp004135

Cited by 4 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1a). These doses of ST and CT have previously been shown to induce a maximal fluid accumulation in the porcine small intestine (Grøndahl et al., 1998; Maltbæk et al., 1998). Treatment with indomethacin had no effect on the ST‐ (mean concentration 1.1 × 10 11 ± 9.6 × 10 11 cfu in n =32; N =8) and the CT‐induced (56 μg/loop in n =12; N =8) jejunal fluid accumulation, although a minor, non‐significant effect was observed in the CT‐induced fluid accumulation.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Indomethacin on Salmonella Typhimurium‐ and Cholera Toxin‐induced Fluid Accumulation in the Porcine Small Intestine

Unmack¹,

Hansen²,

Grøndahl³

et al. 2001

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

The effect of the cyclooxygenase and prostaglandin E2 (PGE2) synthesis inhibitor, indomethacin, on the secretory responses induced by Salmonella serotype Typhimurium (ST) and cholera toxin (CT), in the porcine small intestine was investigated. ST (10(10) colony-forming units) and CT (56 micrograms) were instilled in tied-off intestinal loops in young anaesthetized pigs receiving intravenous indomethacin in a total dose of 7.5 mg/kg, or saline. The accumulated fluid in the loops and the luminal content of endogenous secretagogues PGE2 and 5-hydroxytryptamine (5-HT) were measured. ST induced fluid accumulation in the jejunum, whereas CT induced fluid accumulation in the jejunum and ileum. Indomethacin had no effect on the secretory responses. Indomethacin had a significant effect on the luminal content of PGE2 in jejunal ST and CT loops, whereas no effect of indomethacin was observed on the luminal content of 5-HT in ST and CT loops. In ST and CT loops, an increased content of PGE2 and 5-HT compared with test loops infused with Ringer's solution was observed. These results indicate that the porcine jejunal secretory response to ST and CT does not involve prostaglandins although indomethacin has an influence on the luminal release of PGE2 but not of 5-HT.

show abstract

Section: Resultsmentioning

confidence: 99%

“…(1998). The fluid accumulation to CT in the porcine small intestine depends on the anaesthetic (Maltbæk et al., 1998). Apart from different effects on the nervous system and the synaptic mechanisms (Griffiths and Norman, 1993), anaesthetics additionally influence the endogenous production of PGE 2 (Denson et al., 1996).…”

Section: Discussionmentioning

confidence: 99%

Effects of Indomethacin on Salmonella Typhimurium‐ and Cholera Toxin‐induced Fluid Accumulation in the Porcine Small Intestine

Unmack¹,

Hansen²,

Grøndahl³

et al. 2001

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

show abstract

Neuronal involvement in the effect of an antisecretory factor-derived peptide on induced secretion in the porcine small intestine

Ml¹,

Sørensen²,

Ma³

et al. 2002

Journal of Comparative Physiology A: Sensory, Neural, and Behav

View full text Add to dashboard Cite

The antisecretory factor is a protein inhibiting enterotoxin-induced intestinal inflammation and hypersecretion. We studied the signaling pathway of three antisecretory factor-derived peptides (A1, A3 and A4) in the proximal and distal porcine small intestine. In vivo (ligated loops), only A3 significantly reduced the cholera toxin-induced fluid accumulation and only in proximal loops. A3 and A4 reduced Escherichia coli heat-labile enterotoxin-induced fluid accumulation in the proximal segment, whereas A1 and A3 reduced the Escherichia coli heat-labile enterotoxin-induced fluid accumulation in the distal segment. The secretory response to intraluminally added 5-hydroxytryptamine was not significantly inhibited by the peptides. The amount of intraluminal 5-hydroxytryptamine accumulated in cholera toxin-stimulated loops from the proximal segment was significantly reduced by A3. In vitro,the effect of A3 on secretagogue-induced increases in short-circuit current was recorded from proximal small intestine by the Ussing chamber technique. A3 decreased the tetrodotoxin sensitive effect of substance P. The in vivo results suggest that the antisecretory effect may involve inhibition of the local release of 5-hydroxytryptamine induced by cholera toxin, but not inhibition of secretory reflexes induced by 5-hydroxytryptamine. The in vitro results suggest that the effect of A3 lies beyond the surface epithelium, and involves mucosal neuronal structures.

show abstract

Effects of nitric oxide in 5-hydroxytryptamine-, cholera toxin-, enterotoxigenic Escherichia coli- and Salmonella Typhimurium-induced secretion in the porcine small intestine

Grøndahl¹,

Unmack²,

Ragnarsdóttir³

et al. 2005

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

Secretory response to cholera toxin in the porcine jejunum under different types of general anaesthesia

Cited by 4 publications

References 18 publications

Effects of Indomethacin on Salmonella Typhimurium‐ and Cholera Toxin‐induced Fluid Accumulation in the Porcine Small Intestine

Effects of Indomethacin on Salmonella Typhimurium‐ and Cholera Toxin‐induced Fluid Accumulation in the Porcine Small Intestine

Neuronal involvement in the effect of an antisecretory factor-derived peptide on induced secretion in the porcine small intestine

Effects of nitric oxide in 5-hydroxytryptamine-, cholera toxin-, enterotoxigenic Escherichia coli- and Salmonella Typhimurium-induced secretion in the porcine small intestine

Contact Info

Product

Resources

About