Secretome of the Biocontrol Agent<i>Metarhizium anisopliae</i>Induced by the Cuticle of the Cotton Pest<i>Dysdercus peruvianus</i>Reveals New Insights into Infection

Beys‐da‐Silva, Walter O.; Santi, Lucélia; Berger, Markus; Calzolari, Diego; Passos, Dario Oliveira; Guimarães, Jorge A.; Moresco, James J.

doi:10.1021/pr401204y

Cited by 34 publications

(37 citation statements)

References 72 publications

(180 reference statements)

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“…This result was also reflected in our proteomic analysis. The identification and validation of known enzymes in the context of the interaction of pathogen/parasite with its specific host supports the use of the experimental design presented here and elsewhere 107 .…”

Section: Resultssupporting

confidence: 68%

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

Buffon¹,

Blasi²,

Adamski³

et al. 2015

J. Proteome Res.

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Infestation of phytophagous mite Schizotetranychus oryzae in rice causes critical yield losses. To better understand this interaction, we employed Multidimensional Protein Identification Technology (MudPIT) approach to identify differentially expressed proteins. We detected 18 unique proteins in control and 872 in infested leaves, respectively, along with 32 proteins more abundant in control leaves. S. oryzae infestation caused decreased abundance of proteins related to photosynthesis (mostly photosystem II-related), carbon assimilation and energy production, chloroplast detoxification, defense, fatty acid and gibberellin synthesis. On the other hand, infestation caused increased abundance of proteins involved in protein modification and degradation, gene expression at the translation level, protein partitioning to different organelles, lipid metabolism, actin cytoskeleton remodeling, and synthesis of jasmonate, amino acid and molecular chaperones. Our results also suggest that S. oryzae infestation promotes cell wall remodeling and interferes with ethylene biosynthesis in rice leaves. Proteomic data were positively correlated with enzymatic assays and RT-qPCR analysis. Our findings describe the protein expression patterns of infested rice leaves, and suggest that the acceptor side of PSII is probably the major damaged target in the photosynthetic apparatus. These data will be useful in future biotechnological approaches aiming to induce phytophagous mite resistance in rice.

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Section: Resultssupporting

confidence: 68%

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

Buffon¹,

Blasi²,

Adamski³

et al. 2015

J. Proteome Res.

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“…Other potential virulence factors active on the insect cuticle were recently identified through a proteomic analysis of the M. anisopliae secretome (76), providing candidate targets for further genomic exploration in L. giganteum. Interestingly, these proteomic analyses also related lipolytic activities on the cuticle with ceramidases (76).…”

Section: Discussionmentioning

confidence: 95%

Transcriptome Analysis of the Entomopathogenic Oomycete Lagenidium giganteum Reveals Putative Virulence Factors

Velasquez

Abiff

Fins

et al. 2014

Appl Environ Microbiol

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A combination of 454 pyrosequencing and Sanger sequencing was used to sample and characterize the transcriptome of the entomopathogenic oomycete Lagenidium giganteum. More than 50,000 high-throughput reads were annotated through homology searches. Several selected reads served as seeds for the amplification and sequencing of full-length transcripts. Phylogenetic analyses inferred from full-length cellulose synthase alignments revealed that L giganteum is nested within the peronosporalean galaxy and as such appears to have evolved from a phytopathogenic ancestor. In agreement with the phylogeny reconstructions, full-length L. giganteum oomycete effector orthologs, corresponding to the cellulose-binding elicitor lectin (CBEL), crinkler (CRN), and elicitin proteins, were characterized by domain organizations similar to those of pathogenicity factors of plantpathogenic oomycetes. Importantly, the L. giganteum effectors provide a basis for detailing the roles of canonical CRN, CBEL, and elicitin proteins in the infectious process of an oomycete known principally as an animal pathogen. Finally, phylogenetic analyses and genome mining identified members of glycoside hydrolase family 5 subfamily 27 (GH5_27) as putative virulence factors active on the host insect cuticle, based in part on the fact that GH5_27 genes are shared by entomopathogenic oomycetes and fungi but are underrepresented in nonentomopathogenic genomes. The genomic resources gathered from the L. giganteum transcriptome analysis strongly suggest that filamentous entomopathogens (oomycetes and fungi) exhibit convergent evolution: they have evolved independently from plant-associated microbes, have retained genes indicative of plant associations, and may share similar cores of virulence factors, such as GH5_27 enzymes, that are absent from the genomes of their plant-pathogenic relatives.

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“…Sorokin 1883 is one of the best known entomopathogenic fungi; it is pathogenic to more than 200 species from different insect orders (Freimoser et al, 2005;Samson et al, 2013) and appropriate for commercial development. This fungus has the ability to directly penetrate the insect cuticle (Schneider et al, 2013) through combinations of mechanical pressure and cuticle-degrading enzymes (Beys-da-Silva et al, 2014). When attaching themselves to the body of a suitable host, conidia produce a germ tube, which through extension and growth give rise to hyphae that penetrate into and grow within the insect and causing its death.…”

Section: Introductionmentioning

confidence: 99%

Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

Barra-Bucarei

Vergara

Cortés

2016

Chilean J. Agric. Res.

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One of the best alternatives to reduce the amount of chemical insecticides released into the environment is biological agents. Metarhizium anisopliae (Metschn.) Sorokin 1883 (Hypocreales: Clavicipitaceae) is an entomopathogenic fungus with great potential as a biological pesticide to biologically control pests. However, the relatively high cost of the substrate needed for its mass production system increases product price and discourages its use. The objective of this study was to optimize the mass production conditions of M. anisopliae for use as a biological control agent using two solid substrates, new parboiled rice (NPR) and recycled parboiled rice (RPR). Conidial production was optimized by the response surface methodology (RSM). The effects of the temperature, time, and molasses variables and the interactions between them (conidia g -1 ) were determined. For the NPR substrate, it was determined that the significant variables were time and temperature, and the interactions were temperature × molasses and temperature × time. For the RPR substrate, the significant variables were temperature and time, and the interactions were time × molasses and temperature × time. Both substrates obtained the highest industrial yields at 25 °C for a period of 20 d. Given that the percentage of molasses was not critical for yields, it is recommended that it be set at 5% to reduce costs. Finally, it was possible to use the RPR substrate from the M. anisopliae produ9ction itself as an alternative to solid substrate; mean industrial performance (conidia g -1 ) was higher than values obtained with NPR and at a lower cost.

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Secretome of the Biocontrol AgentMetarhizium anisopliaeInduced by the Cuticle of the Cotton PestDysdercus peruvianusReveals New Insights into Infection

Cited by 34 publications

References 72 publications

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

Transcriptome Analysis of the Entomopathogenic Oomycete Lagenidium giganteum Reveals Putative Virulence Factors

Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

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