Secretion of Proteinases with Fibrinolytic Activity by Micromycetes of the Genus Aspergillus

Osmolovskiy, A. A.; Звонарева, Е.С.; Kreyer, V. G.; Баранова, Н. А.; Егоров, Н.С.

doi:10.3103/s0096392518010066

Cited by 5 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Micromycete A. terreus 2 is a producer of extracellular proteolytic enzymes capable of activating prekallikrein via its limited proteolysis and possessing plasmin-like activity. Previous studies of A. terreus 2 were the first to show that extracellular proteases produced by micromycetes are able to activate prekallikrein in human blood plasma, and also established that proteases of micromycetes are also capable to hydrolyze both fibrin and fibrinogen [5,9,16,17]. Activation of prekallikrein makes it possible to consider the protease of this micromycete as a potential diagnostic component for quantity determining of the specified protein in the blood plasma of patients, and the fibrinolytic effect may indicate a potential use as a treatment for superficial microthrombosis and bruising.…”

Section: Introductionmentioning

confidence: 99%

Action of Extracellular Protease of Aspergillus terreus on Human Plasma Hemostasis Proteins

Osmolovskiy

Звонарева

Баранова

et al. 2022

Microbiol. Biotechnol. Lett.

View full text Add to dashboard Cite

Proteolytic enzymes secreted by Aspergillus, as pathogenicity factors, affect blood coagulation and fibrinolysis, and therefore the target proteins of their action in the bloodstream are of significant interest. In the present study, the action of the isolated protease of A. terreus 2 on different human plasma proteins was shown. The protease of A. terreus 2 exhibited the highest proteolytic activity against hemoglobin, which was 2.5 times higher than the albuminolytic activity shown in both of the protein substrates used. In addition, the protease has significant ability to hydrolyze both fibrin and fibrinogen. However, the inability of the A. terreus 2 protease to coagulate rabbit blood plasma and coagulate human and bovine fibrinogen indicates the severity of the enzyme's action on human blood coagulation factors. It should be considered as a potential indicator of this isolated protease's participation in fungal pathogenesis. The protease shows no hemolytic activity. Furthermore, its activity is insignificantly inhibited by thrombin inhibitors, and is not inhibited by plasmin inhibitors.

show abstract

Section: Introductionmentioning

confidence: 99%

Action of Extracellular Protease of Aspergillus terreus on Human Plasma Hemostasis Proteins

Osmolovskiy

Звонарева

Баранова

et al. 2022

Microbiol. Biotechnol. Lett.

View full text Add to dashboard Cite

show abstract

Biological activity of extracellular protease preparations of Aspergillus ochraceus micromycete on the Paramecium caudatum model

Shabunin

Востроилова

Хохлова

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The research was carried out to study the biological activity of proteases of filamentous fungi, in particular the recombinant protease of Aspergillus ochraceus BMKF41040 micromycete, on a biological model of a unicellular organism - a culture of Paramecium caudatum ciliates. The test object Paramecium caudatum is characterized by a short life cycle, rapid reproduction, it is a convenient model for studying the response of an organism to the effects of preparations. The experiment consisted of 3 stages: rapid assessment of the biological activity of the recombinant protease of Aspergillus ochraceus BMKF41040 micromycete on the culture of infusoria in the stationary growth phase; the assessment of the biological effect of the studied object on the mechanisms of adaptation and cell resistance under the damaging effect of an unfavorable factor; the assessment of the biological activity of the studied objects by the reproduction intensity of ciliates. The results of the studies have shown that the recombinant protease of Aspergillus ochraceus BMKF41040 micromycete does not have a negative and stimulating effect on ciliates and does not affect their reproduction rate. It exhibits moderate biological activity in the test with the action of an unfavorable factor.

show abstract

The Properties of Extracellular Proteinase, an Activator of Blood Plasma Prekallikrein Produced by Aspergillus terreus 2 Micromycete

Zvonareva,

Osmolovskiy,

Baranova

et al. 2023

Appl Biochem Microbiol

View full text Add to dashboard Cite

Secretion of Proteinases with Fibrinolytic Activity by Micromycetes of the Genus Aspergillus

Cited by 5 publications

References 13 publications

Action of Extracellular Protease of Aspergillus terreus on Human Plasma Hemostasis Proteins

Action of Extracellular Protease of Aspergillus terreus on Human Plasma Hemostasis Proteins

Biological activity of extracellular protease preparations of Aspergillus ochraceus micromycete on the Paramecium caudatum model

The Properties of Extracellular Proteinase, an Activator of Blood Plasma Prekallikrein Produced by Aspergillus terreus 2 Micromycete

Contact Info

Product

Resources

About