Secretion of Protein by the Submandibular Glands of the Rat, Mouse, and Hamster in Response to Various Parasympatho- and Sympatho-mimetic Drugs

Abe, Kimio

doi:10.1177/00220345820610121701

Cited by 40 publications

(6 citation statements)

References 16 publications

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“…In agreement with other studies (Wallace and Partlow, 1976;Martinez and Cassity, 1982;Abe and Dawes, 1982;Jones and Wilson, 1985), saliva samples elicited by PILO contained, on the average, less protein than did those elicited by IPR. However, in contrast to our observations, the study that compared the protein concentrations of EPI-and IPR-stimulated saliva samples found that the protein concentration was higher in the EPI samples (Abe and Dawes, 1982). Comparing the two studies suggests the possibility that there is an inverse relationship between treatment dosages of EPI and IPR and protein concentration in saliva.…”

Section: Resultssupporting

confidence: 92%

“…In selected patients, xerostomia has been effectively treated with the secretagogue, pilocarpine (Fox et al, 1986). However, saliva samples produced by systemically administered pilocarpine are generally low in total protein and glycoprotein content (Wallace and Partlow, 1976;Martinez and Cassity, 1982;Abe and Dawes, 1982;Jones and Wilson, 1985). The effect of pilocarpine stimulation on the concentration of specific mucins in saliva is not known.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Various Secretagogues on the Mucin Content of Pure Submandibular Salivas

Denny

Yim

1987

J Dent Res

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The concentrations of a specific mucin and total protein were compared in pure submandibular saliva samples elicited by pilocarpine (PILO), epinephrine (EPI), and isoproterenol (IPR) in mice. At the dosages employed, IPR-stimulated samples contained, on the average, the highest concentrations of protein and mucin. EPI samples contained intermediate levels, and PILO the lowest concentrations. The average mucin-to-protein ratio in the PILO saliva samples was intermediate between the IPR and EPI samples. Non-stimulated saliva samples showed a broader range of mucin to total protein than did the stimulated saliva sets. Within the PILO saliva samples, there was a very strong positive correlation between the mucin content of saliva and the mucin content in the pre-secretory gland prior to stimulation. The mucin content of EPI and IPR samples also showed strong positive relationships with the content of mucin in the gland. The mucin and protein in EPI saliva samples were significantly correlated in at least one of the collections, whereas in the IPR samples, there was a much weaker relationship. With the exception of the third sequential collection of EPI saliva, all nine of the other EPI, IPR, and PILO collection sets showed only a weakly negative or no correlation between flow rate and saliva mucin or protein content. This is in sharp contrast with the strongly negative correlation which was noted between saliva protein or mucin contents and flow rate when all of the data were combined. These observations suggest that the nature of the stimulant is a very important determinant of overall flow rates, saliva protein, and mucin concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Effects of Various Secretagogues on the Mucin Content of Pure Submandibular Salivas

Denny

Yim

1987

J Dent Res

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“…2) and proteins (Table 2) by ad ministration of both doses of metaraminol than pretreatments with only one type of antagonist. It is well-known that activation of j3-adrenoceptors causes the secretion of a small volume of saliva that is rich in protein (15) and mucin (16), whereas activation of a-adrenoceptors causes the secretion of a moderate volume of saliva with little protein (15). Considering these facts and our results described above, it appears that although metaraminol stimulates both a and /3 adrenoceptors, the prominent receptor me diating the secretion of fluid and proteins are (3-adrenoceptors in the case of small doses and a-adrenoceptors in the case of large doses.…”

Section: Discussionmentioning

confidence: 99%

Effects of metaraminol on the secretion of fluid and glycoproteins from the rat submandibular gland.

1989

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Abstract-The actions of metaraminol on the secretion of fluid and glycoproteins from rat submandibular glands were investigated using phentolamine, propranolol and reserpine. Metaraminol at doses from 1 to 8 mg/kg (i.p.) increased the salivation and the amounts of protein in submandibular saliva in a dose-dependent manner. The salivation induced by metaraminol at 2 mg/kg was inhibited strongly by pre treatment with propranolol, whereas the salivation induced by metaraminol at 8 mg/ kg was inhibited strongly by phentolamine.Reserpine inhibited the secretion of fluid caused by both doses of metaraminol. The electrophoretic profiles of saliva evoked by metaraminol at 2 mg/kg revealed two main bands of glycoprotein, I and IV, which originated from the acinus, and the intensities of these bands were de creased by treatment with propranolol, whereas the major band in saliva induced by 8 mg/kg of metaraminol was glycoprotein III, which originated from the granular tubules. The intensity of band III was decreased by pretreatment with phen tolamine. These results suggest that metaraminol, at small doses, stimulates mainly the /3-adrenoceptor in the acinus, whereas at large doses, it prominently stimulates the a-adrenoceptors in the granular tubules, although metaraminol at small and large doses is able to stimulate a and 8-adrenoceptors in rat sub mandibular gland.

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“…Early qualitative studies, in which proteins were separated using native polyacrylamide gels, suggested that the types and proportions of proteins secreted after stimulation with a-or fJ-adrenoceptor or muscarinic cholinoceptor agonists were the same in rat parotid gland (Abe & Dawes, 1978) and mouse and hamster mandibular gland (Abe & Dawes, 1982) but not in the rat mandibular gland (Abe & Dawes, 1978;Abe, Yoneda, Fujita, Yokota & Dawes, 1980). Later studies using SDS gradient gel electrophoresis suggested that isoprenaline-induced rat mandibular saliva contained larger quantities of high molecular weight proteins than saliva evoked by phenylephrine or methacholine (Jones & Wilson, 1985).…”

Section: Salivar Y Secretion Protein Secretionmentioning

confidence: 99%

Effects of acetylcholine, isoprenaline and forskolin on electrolyte and protein composition of rabbit mandibular saliva.

Case

Howorth

Padfield

1988

The Journal of Physiology

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SUMMARY1. The major purpose of this study was to investigate cellular regulation of the ductal transport processes in salivary glands which act to modify the electrolyte composition of primary saliva and cause it to become hypotonic. This was achieved using an isolated mandibular gland preparation by observing the effect of different stimuli on the electrolyte composition of saliva secreted at the same flow rate, on the assumption that these stimuli do not influence primary saliva composition. The effects of the same stimuli on the volume of primary fluid secretion and on protein secretion were also observed. Proteins were measured in total and as individual components after their separation by high-performance liquid chromatography.2. Acetylcholine was used as a 'Ca2+-mobilizing' agonist (i.e. one which both elevates intracellular Ca21 concentration and activates protein kinase C). Isoprenaline was initially used to elevate intracellular cyclic AMP concentration but was subsequently abandoned in favour of forskolin.3. Acetylcholine was a very potent stimulus of primary fluid secretion. By contrast, isoprenaline and forskolin were essentially without effect, even when superimposed on acetylcholine stimulation. 4. As judged by saliva electrolyte composition, increasing the concentration of acetylcholine enhanced ductal absorption of Na+ and Cl-and secretion of K+ (and presumably HCO3. Forskolin had the opposite effect: when superimposed on submaximal acetylcholine stimulation it caused saliva concentrations of Na+ and Cl-to remain high and K+ low (i.e. it inhibited ductal transport processes). The inhibitory effect of forskolin on ductal transport could be overcome by increasing the concentration of acetylcholine, and vice versa.5. Acetylcholine, isoprenaline and forskolin each increased salivary protein secretion, although the kinetics of secretion differed. The spectrum of proteins secreted in response to the three stimuli was the same. The relative proportions of the individual proteins was influenced by the strength of stimulation (i.e. the proportions at high total protein output differed from those at low total protein output) but not apparently by the nature of the stimulus.6. Thus, the three major secretory processes in the rabbit mandibular salivary gland respond differently to the two major signal transduction mechanisms. For R. M. CASE, A. J. HO WORTH AND P. J. PADFIELD primary fluid secretion, Ca2" is stimulatory and cyclic AMP almost without effect; for ductal transport, Ca2" is stimulatory and cyclic AMP inhibitory; and for protein secretion both Ca2+ and cyclic AMP are stimulatory.

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Secretion of Protein by the Submandibular Glands of the Rat, Mouse, and Hamster in Response to Various Parasympatho- and Sympatho-mimetic Drugs

Cited by 40 publications

References 16 publications

The Effects of Various Secretagogues on the Mucin Content of Pure Submandibular Salivas

The Effects of Various Secretagogues on the Mucin Content of Pure Submandibular Salivas

Effects of metaraminol on the secretion of fluid and glycoproteins from the rat submandibular gland.

Effects of acetylcholine, isoprenaline and forskolin on electrolyte and protein composition of rabbit mandibular saliva.

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