beta1,4-N-acetylgalactosaminyltransferase III (beta4GalNAc-T3), which was recently cloned and identified, exhibits GalNAc transferase activity toward a GlcNAcbeta residue with beta1,4-linkage, forming the N,N'-diacetyllactosediamine, GalNAcbeta1,4GlcNAc (LacdiNAc or LDN). Though LacdiNAc has not been found in the gastric mucosa, a large amount of transcript was detected in our previous study. To increase our knowledge of beta4GalNAc-T3 expression and its product LacdiNAc, we examined the exact localization of beta4GalNAc-T3 in human gastric mucosa using a newly developed antibody, monoclonal antibody (mAb) K1356. This antibody specifically detected the enzyme that transfected the beta4GalNAc-T3 gene into MKN45 cells, and the terminal betaGalNAc epitope yielded on the cell surface was recognized by a lectin, Wisteria floribunda agglutinin (WFA). beta4GalNAc-T3 was localized in the supra-nuclear region of surface mucous cells in gastric mucosa, and WFA positively stained the mucins secreted by the cells. In contrast, in the cells of the glandular compartment in the fundic glands and a few cells in the pyloric glands, beta4GalNAc-T3 was observed in the basolateral position of the nucleus, where no WFA reactivity was detected. The anti-Tn (GalNAcalpha-O-Ser/Thr) antibody staining did not overlap with the WFA staining. By measuring the binding activity of WFA using automated frontal affinity chromatography (FAC), we found WFA to bind most strongly LacdiNAc among the sugar chains examined. Neither beta4GalNAc-T3 nor WFA-positive staining was detected in intestinal metaplastic cells. These results suggest that the supra-nuclear expression of beta4GalNAc-T3 is essential for the formation of LacdiNAc on the surface mucous cells and that LacdiNAc and beta4GalNAc-T3 are novel differentiation markers of surface mucous cells in the gastric mucosa.
Summary: For the purpose of studying the relative position of the mylohyoid line, 156 Japanese mandibles (62 dentulous, 94 edentulous) were measured and comparisons made between specimens. Resorption of bone above the level of the mylohyoid line was recognized but no obvious changes were noted below the level of the mylohyoid line, except in rare cases. A straight pattern of the mylohyoid line was observed most frequently, with the segment of the mylohyoid line between the first and second molars being straight. The mean ratio between the total height of the mandible and the height of the mylohyoid line at the level of mesiolingual cusp of the lower first molar was 1:0.65 in males and 1:0.66 in females. These results will be useful in the fabrication of dental prostheses because the level of the occlusal plane can be estimated for edentulous patients using the proportional data for the the mylohyoid line's position.
A position-sensitive device (PSD) of MOS is presented which uses for the first time the dependence of the phase of lateral photovoltage on the position of light incident upon the device. The MOS-type PSD presented here has the following advantages over the previously-presented lateral photodiodes: (1) Its sensitivity can be changed electrically by the gate-voltage. (2) No compensation is required for the change in irradiation intensity. A resolution of 1 µm has been demonstrated for an AN-SiO2-Si PSD to measure the position of light incidence, and better than submicrons should be possible by improving the phase-measurement.
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