Secretion of progesterone, estradiol-17β, PGE, PGF2α, and pregnancy-specific protein B by 90-day intact and ovariectomized pregnant ewes☆

Weems, Y.S.; Bridges, Phillip J.; LeaMaster, Brad R.; Sasser, R.G.; Vincent, D.L.; Weems, C.W.

doi:10.1016/s0090-6980(99)00034-9

Cited by 15 publications

(8 citation statements)

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“…The regulation of PGE secretion by ovine CL (88−90 dpc) may also be via PSPB, which increased PGE and P 4 secretion [127]. During in vivo studies, plasma PSPB and oestradiol 17β concentration was increased in ovariectomised (OVX) pregnant ewes (90 dpc), but oestradiol increased before PSPB [128]. In vitro studies with placenta slices collected seven days later from the same OVX ewes revealed that secretion of P 4 , PGE, oestradiol 17β and PSPB was increased by 2.7-, 3.6-, 2.2-and 2.4-fold, respectively, when compared to secretion by placenta slices of intact pregnant ewes [128].…”

Section: Potential Function(s) Of the Pag Familymentioning

confidence: 99%

“…During in vivo studies, plasma PSPB and oestradiol 17β concentration was increased in ovariectomised (OVX) pregnant ewes (90 dpc), but oestradiol increased before PSPB [128]. In vitro studies with placenta slices collected seven days later from the same OVX ewes revealed that secretion of P 4 , PGE, oestradiol 17β and PSPB was increased by 2.7-, 3.6-, 2.2-and 2.4-fold, respectively, when compared to secretion by placenta slices of intact pregnant ewes [128]. The authors concluded that oestrogens regulate chorionic secretion of PSPB, which can influence placental secretion of PGE 2 that affect placental secretion of P 4 during mid-pregnancy in sheep.…”

Section: Potential Function(s) Of the Pag Familymentioning

confidence: 99%

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Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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-This review presents a broad overview of chorionic glycoproteins encoded by the Pregnancy-Associated Glycoprotein (PAG) gene family and also serves to illustrate how the recent discovery of the PAG family has contributed to our general knowledge of genome evolution, placental transcription and placental protein expression. The complex and large PAG family is restricted to the Artiodactyla order, although single PAG-like genes have also been identified in species outside the Artiodactyla. The PAGs are members of the aspartic proteinase (AP) superfamily. Unexpectedly, however, some members of the PAG family possess amino acid substitutions within and around the active site that likely render them unable to act as proteinases. This paper summarises the available information regarding biodiversity of PAG gene expression based on cDNA cloning, mRNA localisation studies and the structural organisation of the PAG genes with a particular emphasis on PAG promoters. It also compares available data regarding PAG protein purifications, sequencing and their N-glycodiversity. Finally, it discusses the scientific relevance, possible functional roles of the PAGs and describes possible profitable applications related to the detection of PAG proteins in the blood of pregnant domestic and wild species. chorionic glycoproteins / eutherian PAG family / diagnostic PAG markers

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Section: Potential Function(s) Of the Pag Familymentioning

confidence: 99%

Section: Potential Function(s) Of the Pag Familymentioning

confidence: 99%

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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“…PAGs become detectable in maternal circulation around the time of attachment of the fetal placenta when the trophoblast binucleate cells migrate and fuse to the maternal endometrium forming the feto-maternal syncytium [6]. A few studies have postulated their potential role in pregnancy maintenance [7,8] and immune modulation [9], but their precise function remains elusive [10].…”

Section: Introductionmentioning

confidence: 99%

Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep

Roberts

May²,

Veiga-López

2017

Theriogenology

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The ruminant placenta functions as an endocrine and paracrine organ secreting proteins, growth factors, and steroid hormones, including pregnancy-associated glycoproteins (PAG), proteins with elusive function, which are readily detectable in maternal serum after placental attachment. In sheep, circulating progesterone beyond gestational Day 50 is predominantly of placental origin. The relationship among placental secreted factors, including progesterone, remains uncertain in sheep. The first aim of the study was to determine the relationship between gestational PAG profiles-using two commercial ELISA assays-progesterone, and fetal growth during pregnancy. The second aim of the study was to assess the presence and clearance of PAG in neonatal lambs and peripartum ewes in commercial crossbred sheep. Maternal serum samples were collected during mid-pregnancy and assayed for PAG1, pregnancy-specific protein B (PSPB), and progesterone. Maternal serum samples were also collected weekly starting 1-month prepartum until 10 weeks postpartum. Serum samples from newborns were collected at birth until Day 12 after birth and assayed for PAG1. Circulating maternal PAG1 concentrations steadily increased throughout mid-gestation, whereas PSPB exhibited a bimodal pattern of secretion. A strong positive correlation was observed between progesterone and PAG1 (r = 0.779, P < 0.0001), but not between PSPB and progesterone. No relationship was found between placental factors (PAG1, PSPB, and progesterone) and fetal size. PAG1 concentrations were lower before and after parturition in singleton compared with twin pregnancies (P < 0.05). Maternal PAG1 concentrations began declining at parturition and continued to decline until 10 weeks after parturition (P < 0.05). In newborns, PAG1 concentrations continuously declined in both singleton and twins regardless of sex (P < 0.05) and cleared from newborn serum by 12 days after birth. Our findings reported for the first time how different assays used to determine circulating PAG concentrations display different gestational profiles in sheep and how it allows the differentiation between singleton and multiple pregnancies. In conclusion, the strong correlation between PAG1 and progesterone during gestation reports that PAG1 can be effectively used as a marker of placental function.

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“…These pregnancy-associated glycoproteins can be detected in the maternal circulation during pregnancy. In bovine and ovine luteal cells, pregnancy-associated glycoproteins may indirectly regulate luteal progesterone production by the stimulation of prostaglandin E (PGE) synthesis (Del Vecchio et al, 1995;Weems et al, 1999). In bovine endometrium these glycoproteins induce the release of granulocyte chemotactic protein-2 (Austin et al, 1999).…”

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confidence: 99%

Binucleate trophoblast giant cells in the water buffalo (Bubalus bubalis) placenta

Carvalho¹,

Klisch²,

Miglino³

et al. 2005

Journal of Morphology

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The binucleate trophoblast giant cells (BNC) of the water buffalo, Bubalus bubalis, placenta were studied, with emphasis on the synthesis of BNC-specific proteins. Placentomal tissues of 27 water buffalos (2-10 months of pregnancy) were processed for light and electron microscopy. The frequency of BNCs was 20% of the trophoblastic cells in 2-3-month placentas and increased to 27% in the later stages. Ultrastructurally, binucleate cells displayed a prominent granular endoplasmic reticulum and Golgi apparatus, typical of cells involved with protein synthesis and exportation. The buffalo BNCs contained periodic acid-Schiff (PAS)-positive granules and reacted with antisera against bovine placental lactogen, prolactin-related protein-I, and pregnancy-associated glycoproteins. Lectin histochemistry with Dolichos biflorus agglutinin, Vicia villosa agglutinin, and Phaseolus vulgaris leucoagglutinin showed specific staining of BNCs. Different stages of BNC migration and fusion with uterine epithelial cells were observed. Trinucleate feto-maternal hybrid cells were the typical outcome of cell fusions. These cells underwent degeneration, with typical morphological features of apoptosis. The results revealed a strong homology between water buffalo and cattle BNCs concerning cell morphology, protein expression, glycosylation pattern, and characteristics of cell migration and fusion.

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Secretion of progesterone, estradiol-17β, PGE, PGF2α, and pregnancy-specific protein B by 90-day intact and ovariectomized pregnant ewes☆

Cited by 15 publications

References 24 publications

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep

Binucleate trophoblast giant cells in the water buffalo (Bubalus bubalis) placenta

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