Secretion of a latent neutral proteinase that degrades cartilage proteoglycans by skin and synovial fibroblasts in culture

Huybrechts-Godin, G; Vaes, Gilbert

doi:10.1016/0014-5793(78)81182-x

Cited by 23 publications

(8 citation statements)

References 13 publications

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“…The culture media, serum (heat-inactivated by a 30 min incubation at 56°C), the other special chemicals and the animals (male rabbits of the Termonde White Strain, aged 1-2 months) were from the same suppliers as mentioned in our previous studies (Hauser & Vaes, 1978a,b, 1979Huybrechts-Godin & Vaes, 1978). Microtest II culture plates were from Falcon Plastics (Los Angeles, CA, U.S.A.), and Multiwell plates from Costar (Cambridge, MA, U.S.A.).…”

Section: Animals and Materialsmentioning

confidence: 99%

“…Rabbit fibroblasts, growing out from skin explants, were collected after 3 weeks of culture, washed and resuspended in serum-free basal culture medium as previously described (Huybrechts-Godin & Vaes, 1978). Unless otherwise indicated, these experiments were done with fibroblasts collected after their first passage.…”

Section: Cuiltures Of Macrophages Andfibroblastsmentioning

confidence: 99%

“…In previous studies, we have shown that rabbit macrophages or fibroblasts, when cultivated together with pieces of cartilage in a serum-free medium, are both independently capable of degrading the proteoglycan of cartilage, by secreting a metal-dependent neutral proteinase active on the peptide core of the proteoglycan subunits (Vaes et al, 1977;Hauser & Vaes, 1978a;Huybrechts-Godin & Vaes, 1978). Moreover, soluble factors released by rabbit spleen cells upon stimulation with either mitogen or antigen markedly enhance the secretion of the proteinase by the macrophages (Hauser & Vaes, 1979).…”

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confidence: 99%

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Macrophage-fibroblast interactions in collagenase production and cartilage degradation

Huybrechts-Godin¹,

Hauser²,

Vaes³

1979

Biochemical Journal

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Rabbit bone-marrow macrophages and fibroblasts were cultured, independently or together, with pieces of 35S-labelled cartilage or at the surface of dried [14C]collagen gels. Each type of cell, cultivated alone, rapidly degraded the proteoglycan of cartilage, but only the fibroblasts degraded collagen. The co-culture of both types of cell had no consistent effect on the rate of proteoglycan degradation, but it stimulated the rate of collagen degradation. In parallel, the accumulation of collagenase in the culture fluid was enhanced but not that of neutral proteinase. Coinditioned media from macrophage cultures added to cultures of fibroblasts had the same effect as the living macrophages in stimulating the production of collagenase. Their action was itself enhanced when the macrophages had been activated by concanavalin A-stimulated spleen-cell factors. These data suggest that fibroblasts may act as effector cells in producing collagenase and degrading collagen in response to soluble factors released by macrophages under the control of lymphocyte factors.

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Section: Animals and Materialsmentioning

confidence: 99%

Section: Cuiltures Of Macrophages Andfibroblastsmentioning

confidence: 99%

mentioning

confidence: 99%

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Macrophage-fibroblast interactions in collagenase production and cartilage degradation

Huybrechts-Godin¹,

Hauser²,

Vaes³

1979

Biochemical Journal

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“…Fibroblasts from rabbit skin explants were isolated and cultured as previously described [6]. Peritoneal macrophages were obtained from male rabbits (3-4 kg, of the Termonde White strain) that had been injected intraperitoneally with 30 ml of sterile light mineral oil (Merck, Darmstadt, FRG) 4 days earlier.…”

Section: Methodsmentioning

confidence: 99%

Macrophages stimulate the activation of plasminogen by fibroblasts

Laub

Vaes

1982

FEBS Letters

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“…In addition to collagenase rheumatoid synovial tissues release other proteases including three neutral proteases [37,40,98] and cathepsin D [2]. Of most interest to this review is the neutral protease present in synovial membrane as well as in polymorphonuclear leukocytes.…”

Section: Non-immunoglobulin Products Of the Synoviummentioning

confidence: 99%

Protein synthesis in rheumatoid synovial tissue

1981

Springer Semin Immunopathol

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1. Function of synoviocytes and other cells in the synovium A. Histologic Considerations 1. Electron microscopic studies 2. In vivo and in vitro phagocytosis studies 3. Fluorescent antibody staining B. Culture techniques 1. Problems posed by study of isolated cells 2. Long-term explant cultures 3. Advantages of short-term incubations of synovial fragments 4. Isolation of immunoglobulins C. Non-Immunoglobulin Products of the Synovium 1. Products of normal synovium 2. Alterations induced by rheumatoid arthritis II. The Local Immune response in Rheumatoid Synovitis A. Evidence for Active Immune Stimulation 1. Meditators of cellular immunity in synovial fluid 2. Effect of synovectomy 3. Type and amount of immunoglobulin produced B. Local Commitment of Antibody Response 1. Effect of exogenous immunization 2. Rheumatoid factors. 3. Pepsin agglutinators C. 1. Relative enrichment for IgG-3 subclass 2. Increase in lambda-light chain composition III. Pathogenetic Considerations in Rheumatoid Arthritis A. Comparison of Rheumatoid versus Experimental Immune Synovitis 1. Chronic synovitis as a local immune response. 2. Role of cartilage complexes in substaining chronic synovitis B. Significance of the Restriction in the Immunoglobulin Response in Rheumatoid Arthritis 1. Analogy with other disease states in man 2. Common antigen in RA?

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Secretion of a latent neutral proteinase that degrades cartilage proteoglycans by skin and synovial fibroblasts in culture

Cited by 23 publications

References 13 publications

Macrophage-fibroblast interactions in collagenase production and cartilage degradation

Macrophage-fibroblast interactions in collagenase production and cartilage degradation

Macrophages stimulate the activation of plasminogen by fibroblasts

Protein synthesis in rheumatoid synovial tissue

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