Secretion by Micromycetes of Proteases with Activity Similar to the Activity of the Hemostatic System Proteins

Lukianova, Anna A.; Kornienko, E. I.; Vigand, P. A.; Kreyer, V. G.; Кураков, А. В.; Osmolovskiy, A. A.

doi:10.3103/s009639252001006x

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…DJ-4 was 3.97 and 2.67-fold higher than subtilisin Carlsberg and subtilisin BPN, correspondingly [37]. For some filamentous fungi, it was shown that the ratio of overall proteolytic (non-specific) activity to fibrinolytic activity is one of the effective fibrin hydrolysis criteria and it is within the values 0.18-0.57 [186].…”

Section: Physicochemical Characterisation Of Microbial Fibrinolytic Enzymesmentioning

confidence: 94%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

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Cardiac disorders such as acute myocardial infarction, embolism and stroke are primarily attributed to excessive fibrin accumulation in the blood vessels, usually consequential in thrombosis. Numerous methodologies including the use of anti-coagulants, anti-platelet drugs, surgical operations and fibrinolytic enzymes are employed for the dissolution of fibrin clots and hence ameliorate thrombosis. Microbial fibrinolytic enzymes have attracted much more attention in the management of cardiovascular disorders than typical anti-thrombotic strategies because of the undesirable after-effects and high expense of the latter. Fibrinolytic enzymes such as plasminogen activators and plasmin-like proteins hydrolyse thrombi with high efficacy with no significant after-effects and can be cost effectively produced on a large scale with a short generation time. However, the hunt for novel fibrinolytic enzymes necessitates complex purification stages, physiochemical and structural-functional attributes, which provide an insight into their mechanism of action. Besides, strain improvement and molecular technologies such as cloning, overexpression and the construction of genetically modified strains for the enhanced production of fibrinolytic enzymes significantly improve their thrombolytic potential. In addition, the unconventional applicability of some fibrinolytic enzymes paves their way for protein hydrolysis in addition to fibrin/thrombi, blood pressure regulation, anti-microbials, detergent additives for blood stain removal, preventing dental caries, anti-inflammatory and mucolytic expectorant agents. Therefore, this review article encompasses the production, biochemical/structure-function properties, thrombolytic potential and other surplus applications of microbial fibrinolytic enzymes.

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Section: Physicochemical Characterisation Of Microbial Fibrinolytic Enzymesmentioning

confidence: 94%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

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“…Extracellular proteolytic enzymes of micromycetes can act on haemostatic system proteins as activators or direct fibrinolytic agents [3,4]. The proteases of Sarocladium strictum 203 (VKM F-4845D) showed a direct fibrinolytic activity and a plasminogen-activating effect (indirect fibrinolytic activity) [5]. In this regard, the study of the properties of proteases from this producer is essential.…”

Section: Introductionmentioning

confidence: 99%

Acute Toxicity, Immunotoxicity and Allergenicity of Protease Complex Obtained from Micromycete Sarocladium strictum

et al. 2021

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The different effects on animals of the thrombolytic protease complex of the new producer S. strictum 203 were studied. The tests of acute toxicity, immunotoxicity and allergenicity should conclude that the studied proteolytic complex is safe for medical usage. For the intravenous and the intraperitoneal routes of administration, the maximum tolerated dose and the median lethal dose were not determined.

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Characteristics and Properties of the Complex of Proteolytic Enzymes of the Thrombolytic Action of the Micromycete Sarocladium strictum

Kornienko

Osmolovskiy

Kreyer

et al. 2021

Appl Biochem Microbiol

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Secretion by Micromycetes of Proteases with Activity Similar to the Activity of the Hemostatic System Proteins

Cited by 6 publications

References 18 publications

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

Acute Toxicity, Immunotoxicity and Allergenicity of Protease Complex Obtained from Micromycete Sarocladium strictum

Characteristics and Properties of the Complex of Proteolytic Enzymes of the Thrombolytic Action of the Micromycete Sarocladium strictum

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