Characteristics and Properties of the Complex of Proteolytic Enzymes of the Thrombolytic Action of the Micromycete Sarocladium strictum

Kornienko, E. I.; Osmolovskiy, A. A.; Kreyer, V. G.; Баранова, Н. А.; Kotova, Irina; Егоров, Н.С.

doi:10.1134/s0003683821010129

Cited by 11 publications

(10 citation statements)

References 26 publications

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“…It is well known that proteolytic enzymes of Aspergillus have a high potential for use as therapeutic agents as components of thrombolytic drugs and in the diagnosis of diseases of the hemostasis system as protein activators of proenzymes [ 33 , 34 , 35 ]. The obtained data allow us to judge the possibility of proteases of studied strains used as direct thrombolytics capable of cleaving blood clots in a few hours.…”

Section: Resultsmentioning

confidence: 99%

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Osmolovskiy

Schmidt

Orekhova

et al. 2021

Life

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In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active in cleaving both globular (albuminolysis) and fibrillar (fibrin) proteins, and, to varying degrees, they could coagulate the plasma of humans and animals (due to proteolysis of factors of the blood coagulation cascade) but were not able to coagulate fibrinogen. The proteases of both Aspergillus fully hydrolyzed thrombi in 120–180 min. Micromycetes did not show hemolytic activity but were able to break down hemoglobin.

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Section: Resultsmentioning

confidence: 99%

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Osmolovskiy

Schmidt

Orekhova

et al. 2021

Life

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“…Many micromycetes are well known as producers of proteases targeting proteins of the haemostatic system. So, in addition to the fibrinolytic properties of such proteases, the ability of some to activate a number of blood coagulation factors, such as plasminogen [ 47 , 48 ], protein C [ 11 ], prothrombin [ 49 ], prekallikrein [ 50 ], and factor X [ 51 ], is known. Proteolytic enzymes of these micromycetes are capable of cleaving a characteristic range of chromogenic peptide substrates of proteins of the haemostasis system and demonstrate differences in the activity with respect to globular and fibrillar proteins.…”

Section: Discussionmentioning

confidence: 99%

Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete Aspergillus ochraceus Capable of Activating Protein C of Blood Plasma

et al. 2021

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Micromycetes are known to secrete numerous enzymes of biotechnological and medical potential. Fibrinolytic protease-activator of protein C (PAPC) of blood plasma from micromycete Aspergillus ochraceus VKM-F4104D was obtained in recombinant form utilising the bacterial expression system. This enzyme, which belongs to the proteinase-K-like proteases, is similar to the proteases encoded in the genomes of Aspergillus fumigatus ATCC MYA-4609, A. oryzae ATCC 42149 and A. flavus 28. Mature PAPC-4104 is 282 amino acids long, preceded by the 101-amino acid propeptide necessary for proper folding and maturation. The recombinant protease was identical to the native enzyme from micromycete in terms of its biological properties, including an ability to hydrolyse substrates of activated protein C (pGlu-Pro-Arg-pNA) and factor Xa (Z-D-Arg-Gly-Arg-pNA) in conjugant reactions with human blood plasma. Therefore, recombinant PAPC-4104 can potentially be used in medicine, veterinary science, diagnostics, and other applications.

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“…Proteases with plasminogen activating activity were found in representatives of micromycetes of different systematic and ecological-trophic groups: Arthrobotrys longa [60], Tolypocladium inflatum [61], Rhizopus microsporus var. tuberosus [62], Neurospora sitophila [63] and Sarocladium strictum [64].…”

Section: Microbial Fibrinolytic Enzymes: Production Status and Diversitymentioning

confidence: 99%

“…Another method for in-laboratory purification of fibrinolytic enzymes is isoelectrofocusing. The method was used for obtaining pure enzymes for their characterisation and further special studies [20,30,32,64,99]. Isoelectrofocusing allows one not only to isolate enzymes, but also to get a primary idea of their properties and compare fibrinolytic enzymes from different producers.…”

Section: Recovery and Purification Of Fibrinolytic Enzymesmentioning

confidence: 99%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

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Cardiac disorders such as acute myocardial infarction, embolism and stroke are primarily attributed to excessive fibrin accumulation in the blood vessels, usually consequential in thrombosis. Numerous methodologies including the use of anti-coagulants, anti-platelet drugs, surgical operations and fibrinolytic enzymes are employed for the dissolution of fibrin clots and hence ameliorate thrombosis. Microbial fibrinolytic enzymes have attracted much more attention in the management of cardiovascular disorders than typical anti-thrombotic strategies because of the undesirable after-effects and high expense of the latter. Fibrinolytic enzymes such as plasminogen activators and plasmin-like proteins hydrolyse thrombi with high efficacy with no significant after-effects and can be cost effectively produced on a large scale with a short generation time. However, the hunt for novel fibrinolytic enzymes necessitates complex purification stages, physiochemical and structural-functional attributes, which provide an insight into their mechanism of action. Besides, strain improvement and molecular technologies such as cloning, overexpression and the construction of genetically modified strains for the enhanced production of fibrinolytic enzymes significantly improve their thrombolytic potential. In addition, the unconventional applicability of some fibrinolytic enzymes paves their way for protein hydrolysis in addition to fibrin/thrombi, blood pressure regulation, anti-microbials, detergent additives for blood stain removal, preventing dental caries, anti-inflammatory and mucolytic expectorant agents. Therefore, this review article encompasses the production, biochemical/structure-function properties, thrombolytic potential and other surplus applications of microbial fibrinolytic enzymes.

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Characteristics and Properties of the Complex of Proteolytic Enzymes of the Thrombolytic Action of the Micromycete Sarocladium strictum

Cited by 11 publications

References 26 publications

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins

Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete Aspergillus ochraceus Capable of Activating Protein C of Blood Plasma

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

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