Secretion and functional expression of Mycobacterium bovis antigens MPB70 and MPB83 in lactic acid bacteria

Stedman, Anna; Chambers, Mark A.; Gutierrez-Merino, Jorge

doi:10.1016/j.tube.2019.05.007

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…97 LAB are also ideal candidates for use as safe delivery vectors expressing therapeutic recombinant proteins. 98 Oral administration of probiotics has also been suggested as a convenient and safe way to improve the efficacy of conventional vaccines within communities. 99 All procedures with human blood samples have been approved by the Surrey University Animals Ethics Committee in accordance with the Institutional Policy on the Donation and Use of Human Specimens in Teaching and Research and the national guidelines under which the institution operates.…”

Section: Resultsmentioning

confidence: 99%

“…This would help identify the optimal conditions in which they could work, as recommended by the 2016 FAO report on probiotics in animal nutrition . LAB are also ideal candidates for use as safe delivery vectors expressing therapeutic recombinant proteins . Oral administration of probiotics has also been suggested as a convenient and safe way to improve the efficacy of conventional vaccines within communities …”

Section: Resultsmentioning

confidence: 99%

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Gut commensal bacteria show beneficial properties as wildlife probiotics

Stedman

Vliet

Chambers

et al. 2020

Annals of the New York Academy of Sciences

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Probiotics are noninvasive, environmentally friendly alternatives for reducing infectious diseases in wildlife species.Our aim in the present study was to evaluate the potential of gut commensals such as lactic acid bacteria (LAB) as wildlife probiotics. The LAB selected for our analyses were isolated from European badgers (Meles meles), a wildlife reservoir of bovine tuberculosis, and comprised four different genera: Enterococcus, Weissella, Pediococcus, and Lactobacillus. The enterococci displayed a phenotype and genotype that included the production of antibacterial peptides and stimulation of antiviral responses, as well as the presence of virulence and antibiotic resistance genes; Weissella showed antimycobacterial activity owing to their ability to produce lactate and ethanol; and lactobacilli and pediococci modulated proinflammatory phagocytic responses that associate with protection against pathogens, responses that coincide with the presence of immunomodulatory markers in their genomes. Although both lactobacilli and pediococci showed resistance to antibiotics, this was naturally acquired, and almost all isolates demonstrated a phylogenetic relationship with isolates from food and healthy animals. Our results show that LAB display probiotic benefits that depend on the genus, and that lactobacilli and pediococci are probably the most obvious candidates as probiotics against infectious diseases in wildlife because of their food-grade status and ability to modulate protective innate immune responses. . P. acidilactici E24, P. lolii F7, P. acidilactici I32, P. acidilactici M17, Weissella cibaria A23, W. paramesenteroides A37, and W. paramesenteroides N43. All displayed extracellular activity against Mycobacterium smegmatis at a low pH and reduced the viability and survival rate of M. bovis BCG when competing together in the same broth for 48 hours. 17 In the present study, further species analysis was carried out using the published Genbank genomes from E. faecalis, L. plantarum, L. reuterii, pediococci, and weissella with feature frequency profiling (FFP). 23 This technique allows both cross-species and cross-genus comparisons between genome sequences to confirm bacterial identification. The isolates previously identified as

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Gut commensal bacteria show beneficial properties as wildlife probiotics

Stedman

Vliet

Chambers

et al. 2020

Annals of the New York Academy of Sciences

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“…Additionally, L. lactis was used as expression host alternative to E. coli due to following advantageous properties i) generally recognized as safe (GRAS) microorganism ii) lack of outer membrane (iii) insignificant extracellular proteolysis activity (iv) free of endotoxins (v) no lipo-polysaccharide contamination (vii) accommodates cysteine-rich proteins (vii) accessibility of both inducible and constitutive genetic control systems (viii) able to express prone-toaggregate and/or difficult-to-purify proteins (ix) presentation to the host immune system in the context of micro-particles to avoids the immunotolerance, which is normally provoked by oral delivery of soluble antigens (x) exhibits similar codon bias to P. falciparum, which makes it efficient protein expression and secretion system to outer surface that could easily interact with host immune system [113,[141][142][143]. In recent years, several wet lab studies have confirmed the utilization of L. lactis as an expression host to produce properly folded, pure and stable chimeric and/or single antigenic proteins of many pathogens that elicited high levels of functional antibodies/cytokines including P. falciparum [144][145][146][147][148], Mycobacterium bovis [149], Mycobacterium tuberculosis [150], Helicobacter pylori [151], Polish avian H5H1 influenza [152], cancer [153] and Staphylococcus aureus [154]. Moreover, L. lactismediated delivery of DNA vaccines also lead to the expression of post-translationally modified antigens by host cells resulting in presentation of conformationally restricted epitopes to the immune system for induction of both cellular and humoral immune responses [112].…”

Section: Codon Optimization In Silico Cloning and Expression Of Pv1a And Pv3bmentioning

confidence: 99%

A cutting-edge immunoinformatics approach for design of multi-epitope oral vaccine against dreadful human malaria

Pritam

Singh

Swaroop

et al. 2020

International Journal of Biological Macromolecules

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Human malaria is a pathogenic disease mainly caused by Plasmodium falciparum, which was responsible for about 405,000 deaths globally in the year 2018. To date, several vaccine candidates have been evaluated for prevention, which failed to produce optimal output at various preclinical/clinical stages. This study is based on designing of polypeptide vaccines (PVs) against human malaria that cover almost all stages of life-cycle of Plasmodium and for the same 5 genome derived predicted antigenic proteins (GDPAP) have been used. For the development of a multi-immune inducer, 15 PVs were initially designed using T-cell epitope ensemble, which covered N99% human population as well as linear B-cell epitopes with or without adjuvants. The immune simulation of PVs showed higher levels of T-cell and B-cell activities compared to positive and negative vaccine controls. Furthermore, in silico cloning of PVs and codon optimization followed by enhanced expression within Lactococcus lactis host system was also explored. Although, the study has sound theoretical and in silico findings, the in vitro/in vivo evaluation seems imperative to warrant the immunogenicity and safety of PVs towards management of P. falciparum infection in the future.

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“…Inhibition of the FnBPs-Fn interaction would signi cantly abolish bacterial binding and invasion of host cells, implying that this pathway is crucial for Mycobacterium pathogenesis, so further research is needed [8]. MPB83 is a bacterial surface protein expressed more by M. bovis than by M. tuberculosis, so MPB83 has a promising diagnostic potential compared to other proteins that can bind to integrins [10], [11].…”

Section: Introductionmentioning

confidence: 99%

Molecular Docking MPB83 Protein on β2, β3, and α5β1 Integrins: Pathogenesis of Bovine Tuberculosis in Humans

Rahmani

Rachmania

Mufida

2023

Preprint

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Tuberculosis is a chronic infectious disease caused by the Mycobacterium tuberculosis complex (MTBC). Two MTBC species that causes infection in human are Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis). Bovine tuberculosis (bTB) is a zoonotic disease with 2.55 times higher mortality rate compared to tuberculosis due to M. tuberculosis. Mycobacterium infect humans through the bonding between adhesin molecules on the bacteria surface and fibronectin receptors on the human body such as β2, β3, and α5β1 integrins. β2, β3, and α5β1 integrins are responsible for migrating bacterial activity and strengthening cell adhesion. MPB83 is a bacterial surface protein expressed more by M. bovis than by M. tuberculosis. This study aims to examine the binding of MPB83 protein to β2, β3, and α5β1 integrins as potential pathogenesis of bTB in humans by molecular docking method. Molecular docking was carried out using ClusPro to determine the interaction between the MPB83 protein and β2, β3, and α5β1 integrins based on binding energy and binding interaction model. The results showed that in silico, bonds occur between MPB83 protein and β2, β3, and α5β1 integrins which can potential for the pathogenesis of bTB in humans.

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Secretion and functional expression of Mycobacterium bovis antigens MPB70 and MPB83 in lactic acid bacteria

Cited by 5 publications

References 38 publications

Gut commensal bacteria show beneficial properties as wildlife probiotics

Gut commensal bacteria show beneficial properties as wildlife probiotics

A cutting-edge immunoinformatics approach for design of multi-epitope oral vaccine against dreadful human malaria

Molecular Docking MPB83 Protein on β2, β3, and α5β1 Integrins: Pathogenesis of Bovine Tuberculosis in Humans

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