Secondary Metabolites Production by Actinomycetes and their Antifungal Activity

Dewi, Tirta Kumala; Agustiani, Dwi; Antonius, Sarjiya

doi:10.18502/kls.v3i4.713

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…This could be evidenced by the production of fungichromin, a polyene antifungal compound detected in extracts of infrutescene of Protea. Dewi et al (2017) also proved the antifungal activity of metabolites of Streptomyces spp. against Fusarium oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 90%

Unraveling the tripartite interaction of volatile compounds of Streptomyces rochei with grain mold pathogens infecting sorghum

et al. 2022

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Sorghum is a major grain crop used in traditional meals and health drinks, and as an efficient fuel. However, its productivity, value, germination, and usability are affected by grain mold, which is a severe problem in sorghum production systems, which reduces the yield of harvested grains for consumer use. The organic approach to the management of the disease is essential and will increase consumer demand. Bioactive molecules like mVOC (volatile organic compound) identification are used to unravel the molecules responsible for antifungal activity. The Streptomyces rochei strain (ASH) has been reported to be a potential antagonist to many pathogens, with high levels of VOCs. The present study aimed to study the inhibitory effect of S. rochei on sorghum grain mold pathogens using a dual culture technique and via the production of microbial volatile organic compounds (mVOCs). mVOCs inhibited the mycelial growth of Fusarium moniliforme by 63.75 and Curvularia lunata by 68.52%. mVOCs suppressed mycelial growth and inhibited the production of spores by altering the structure of mycelia in tripartite plate assay. About 45 mVOCs were profiled when Streptomyces rochei interacted with these two pathogens. In the present study, several compounds were upregulated or downregulated by S. rochei, including 2-methyl-1-butanol, methanoazulene, and cedrene. S. rochei emitted novel terpenoid compounds with peak areas, such as myrcene (1.14%), cymene (6.41%), and ç-terpinene (7.32%) upon interaction with F. moniliforme and C. lunata. The peak area of some of the compounds, including furan 2-methyl (0.70%), benzene (1.84%), 1-butanol, 2-methyl-(8.25%), and myrcene (1.12)%, was increased during tripartite interaction with F. moniliforme and C. lunata, which resulted in furan 2-methyl (6.60%), benzene (4.43%), butanol, 2-methyl (18.67%), and myrcene (1.14%). These metabolites were implicated in the sesquiterpenoid and alkane biosynthetic pathways and the oxalic acid degradation pathway. The present study shows how S. rochei exhibits hyperparasitism, competition, and antibiosis via mVOCs. In addition to their antimicrobial functions, these metabolites could also enhance plant growth.

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Section: Discussionmentioning

confidence: 90%

Unraveling the tripartite interaction of volatile compounds of Streptomyces rochei with grain mold pathogens infecting sorghum

et al. 2022

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“…Each filtrate was blotted on Silica gel 60 F264 plate size 10 cm×5 cm (Whatman). Dichloromethane and methanol (9: 1) (v/v) was used as solvent (Dewi et al, 2017). TLC plates were removed from the chamber immediately before the solvent reached 1 cm from the top of the plates.…”

Section: Thin Layer Chromatography (Tlc) Analysismentioning

confidence: 99%

Antifungal Potency of Secondary Metabolites Produced by Endophytic Bacteria against Pathogenic Fungi Pyricularia oryzae Cav.

Widiantini

Qadryani

Hartati

et al. 2019

JPTI

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Blast disease caused by Pyricularia oryzae Cav. is one of the most important diseases on rice. One of the alternative controlling methods in P. oryzae is biological control through the utilization of secondary metabolites produced by endophytic bacteria. The study aimed to determine the antifungal potency of secondary metabolites produced by rice endophytic bacteria against P. oryzae. The experiment was conducted using 9 endophytic bacteria isolated rice (Os1, Os2, Os3, Os4, Os5, Os6, Os7, Os8, and Os10). Each isolates were grown in ISP2 liquid media and the secondary metabolites compounds were extracted using two different solvents; methanol and ethyl acetate : methanol (4:1) (v/v). The effect of secondary metabolites was tested using agar well diffusion method. The results demonstrated that the secondary metabolites extracted by both solvents have antifungal effect on the growth of P. oryzae. The highest growth inhibition was shown by secondary metabolites extracted by ethyl acetate : methanol (4:1) from Os1 (42%) and Os3 (39%). Antifungal activity of the secondary metabolites was indicated by the formation of clear zone. HPLC (High Performance Liquid Chromatography) analysis showed the differences of peaks and retention time between secondary metabolites produced by Os1 and Os3 which has antifungal activity and secondary metabolites produced by Os10 that did not show the antifungal activity.

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“…Most antibiotics developed in the 21st century are based on the Streptomyces secondary metabolites [ 19 , 20 ]. Many Streptomyces have antifungal properties [ 21 , 22 ]. A number of strains have biocontrol potential for Magnaporthe oryzae ( Pyricularia oryzae ), a fungus causing rice blast [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity and Biosynthetic Potential of New Streptomyces sp. MW-W600-10 Strain Isolated from Coal Mine Water

Siupka

Hansen

Schier

et al. 2021

IJMS

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Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus Streptomyces are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new Streptomyces sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens Fusarium culmorum DSM62188 and Nigrospora oryzae roseF7. Furthermore, we analyzed the strain’s biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the Streptomyces sp. S-2 strain isolated from black soot against F. culmorum DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential.

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Secondary Metabolites Production by Actinomycetes and their Antifungal Activity

Cited by 12 publications

References 15 publications

Unraveling the tripartite interaction of volatile compounds of Streptomyces rochei with grain mold pathogens infecting sorghum

Unraveling the tripartite interaction of volatile compounds of Streptomyces rochei with grain mold pathogens infecting sorghum

Antifungal Potency of Secondary Metabolites Produced by Endophytic Bacteria against Pathogenic Fungi Pyricularia oryzae Cav.

Antifungal Activity and Biosynthetic Potential of New Streptomyces sp. MW-W600-10 Strain Isolated from Coal Mine Water

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