Secondary metabolites of Hypericum species from the Drosanthe and Olympia sections

Çırak, Cüneyt; Radušienė, Jolita; Jakštas, Valdas; Ivanauskas, Liudas; Yayla, Fatih; Seyis, Fatih; Camas, Necdet

doi:10.1016/j.sajb.2015.09.022

Cited by 18 publications

(12 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Drosanthe. 25 As an exception, we detected caffeic acid u n c o r r e c t e d p r o o f in low amounts whereas this compound was not found in the other members of the section. 25 Our results provide additional data for the chemotaxonomy of Hypericum genus.…”

Section: U N C O R R E C T E D P R O O Fcontrasting

confidence: 53%

“…20,21 In the present study, it was found that the considerable amount of the phenolic compounds in H. pseudolaeve consisted of the flavonoids, corroborating the previous reports on other Hypericum species. [22][23][24][25][26] Wound healing and anti-depressant activities of the members of this genus have been associated with phenolic compounds such as hyperoside and epicatechin in some of the previous studies. 11,27 Therefore, phenolic content is important for the biological activies of Hypericum sp.…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

“…In general, the secondary metabolites identified in H. pseudolaeve in the present study are in agreement with the previously published literature records. 11,23,25,[33][34][35][36][37][38] Chemical composition of H. pseudolaeve from Turkey was studied by HPLC recently. 23 The authors gave yield (%) values for each compound and did not specify the calculation, making their results not comparable with ours.…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

“…Secondary metabolites such as quercetin, quercitrin, hyperoside and hypericin were considered as useful biomarkers for chemotaxonomic analyses. 25 Chemical profiling can provide additional data for taxonomic classifications based on morphology and genetics. Our results are in concordance with previously published data reporting the chemical constituents of the members belonging to the sect.…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

See 3 more Smart Citations

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

İğci¹,

Aytaç²

2020

tjps

View full text Add to dashboard Cite

GİRİŞ ve AMAÇ: Bu çalışma, Hypericum pseudolaeve Robson türünün iki farklı yöntemle elde edilmiş metanol ve su ekstrelerinin in vitro yara iyileştirici etkisi ile sitotoksisitesi, antioksidan aktivitesi ve seçilmiş fitokimyasallarını araştırmayı amaçlamıştır. YÖNTEM ve GEREÇLER: Toplam fenolik ve flavonoit içerikleri spektrofotometri tabanlı yöntemler kullanılarak ölçülmüştür. Ekstrelerin L929 fare fibroblast hücreleri üzerindeki sitotoksik etkileri MTT deneyi ile değerlendirilmiştir. Ayrıca, bitki uygulanmış fibroblast hücrelerinin göçü, bir in vitro yara iyileşme modeli olan hücre çizik yöntemi ile değerlendirilmiştir. Ek olarak, türün kimyasal içeriği Yüksek Performanslı Sıvı Kromatografisi (YPSK) ile belirlenmiştir. BULGULAR: Sitotoksisite deneyinin sonuçları, metanol ve su ekstrelerinin, 500 µg/mL konsantrasyonuna kadar kullanıldığında fibroblast hücreleri üzerinde herhangi bir sitotoksik etkiye sahip olmadığını göstermiştir. Fibroblast göçü 62 µg/mL konsantrasyonunda su ekstreleri uygulandığında negatif kontrole göre anlamlı derecede artış göstermiştir. Ekstreler iyi derecede antioksidan aktivite göstermiştir ve YPSK analizi ile 16 madde tespit edilmiştir. En fazla bulunan bileşik epikateşin olarak belirlenmiştir. TARTIŞMA ve SONUÇ: Bu çalışma Hypericum pseudolaeve ekstrelerinin, yara iyileşme potansiyeline sahip olduğunu ve çeşitli önemli antioksidan fenolikler içerdiğini göstermiştir. Bu türle ilgili aktif bileşiklerin izole edilmesi ve tanımlamasını amaçlayan daha fazla araştırma yapılmalıdır. Anahtar Kelimeler: Yara iyileşmesi, bitki ekstresi, antioksidan, fenolikler, YPSK INTRODUCTION: This study was aimed to investigate the in vitro wound healing effects of the methanolic and aqueous extracts of Hypericum pseudolaeve Robson obtained by two different methods as well as its cytotoxicity, antioxidant activity and selected phytochemical constituents. METHODS: Total phenolic and flavonoid contents were measured using spectrophotometry based methods. Cytotoxic effects of the extracts on L929 mouse fibroblast cells were evaluated by MTT assay. Moreover, migration and spreading of the treated fibroblast cells were assessed by cell scratch assay as an in vitro wound healing model. In addition, the chemical content of the species was determined by High Performance Liquid Chromatography (HPLC). RESULTS: The results of cytotoxicity assay indicated that the methanolic and aqueous extract did not have any cytotoxic effect on fibroblast cells at concentrations up to 500 µg/mL. Fibroblast migration was significantly increased by 62 µg/mL concentration of the aqueous extracts compared to negative control. Extracts showed good antioxidant activity and 16 phytochemical compounds were detected by HPLC, with the highest amount for u n c o r r e c t e d p r o o f 2 epicatechin. DISCUSSION AND CONCLUSION: Our results showed that H. pseudolaeve extracts have wound healing potential and contain several important antioxidant phenolic compounds. This species deserve further investigation aiming to isolate and identify the activ...

show abstract

Section: U N C O R R E C T E D P R O O Fcontrasting

confidence: 53%

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

See 2 more Smart Citations

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

İğci¹,

Aytaç²

2020

tjps

View full text Add to dashboard Cite

show abstract

“…Hypericin and pseudohypericin were detected only in flowers and leaves had the highest accumulation level of chlorogenic acid in H. scabrum L. and H. bupleuroides Gris. (Cirak et al, 2016). In wild and greenhouse-grown H. triquetrifolium Turra plants, flowers accumulated the highest level of hyperoside, quercetin and quercitrin; however, leaves produced higher amount of chlorogenic acid and isoquercetin (Cirak et al, 2013 a).…”

Section: Resultsmentioning

confidence: 99%

Secondary metabolites in Hypericum species and their distribution in different plant parts

Odabas

Radušienė

Ivanauskas

et al. 2016

Zemdirbyste-Agriculture

Self Cite

View full text Add to dashboard Cite

The genus Hypericum (Hypericaceae) has attracted remarkable scientific interest as its members accumulate significant amounts of various bioactive compounds. In the current study, we investigated accumulation of several bioactive compounds in various parts of Hypericum hircinum L. subsp. majus (Ainton) N. Robson, H. pallens Banks et Sol., H. russeggeri (Fenzl) R. Keller and H. lanuginosum Lam. The plant materials were harvested at flowering, dissected into different tissues and subsequently subjected to high performance liquid chromatography (HPLC) analyses. Accumulation level of the investigated compounds varied greatly depending on species and plant parts. Among different plant tissues, flowers were found to be superior to leaves with respect to accumulation of the chemicals tested except for neochlorogenic, caffeic and 2,4-dihydroxybenzoic acids which were accumulated mainly in leaves. H. hircinum did not produce quercitrin or avicularin, H. lanuginosum did not accumulate hyperforins and caffeic acid, rutin was detected only in H. pallens. For the first time, chemical profiles of these Turkish species of Hypericum were reported and the results are discussed from a phytochemical point of view. The present data could be helpful in selecting the future targets for phytochemical and biological studies on Hypericum genus as well as enriching our current knowledge about Hypericum chemistry.

show abstract

Phenolic Compound Contents of Hypericum Species from Turkey

Asan

2020

Propagation and Genetic Manipulation of Plants

View full text Add to dashboard Cite

Secondary metabolites of Hypericum species from the Drosanthe and Olympia sections

Cited by 18 publications

References 47 publications

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

Secondary metabolites in Hypericum species and their distribution in different plant parts

Phenolic Compound Contents of Hypericum Species from Turkey

Contact Info

Product

Resources

About