Secondary Metabolites of an Endophytic Fungus Phomopsis castaneae-mollissimae

Qian, Yi-Xin; Kang, Ji-Chuan; Luo, Yikai; He, Junyu; Wang, Lu; Li, Qi-Rui

doi:10.1007/s10600-018-2340-0

Cited by 6 publications

(4 citation statements)

References 4 publications

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“…13 C NMR (125 MHz, Methanol- d 4 ): δ C 197.8 (C-4), 168.3 (C-7), 165.5 (C-5), 164.9 (C-9), 159.0 (C-4), 131.1 (C-1’), 129.0 (C-2’, C-6’), 116.3 (C-3’, C-5’), 103.4 (C-10), 97.0 (C-6), 96.1 (C-8), 80.5 (C-2), 44.0 (C-3). The NMR data of 1 correspond well to those of naringenin 51 .…”

Section: Methodssupporting

confidence: 60%

A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin

Zhang

Zhou

et al. 2022

Nat Commun

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Biosynthesis of the flavonoid naringenin in plants and bacteria is commonly catalysed by a type III polyketide synthase (PKS) using one p-coumaroyl-CoA and three malonyl-CoA molecules as substrates. Here, we report a fungal non-ribosomal peptide synthetase -polyketide synthase (NRPS-PKS) hybrid FnsA for the naringenin formation. Feeding experiments with isotope-labelled precursors demonstrate that FnsA accepts not only p-coumaric acid (p-CA), but also p-hydroxybenzoic acid (p-HBA) as starter units, with three or four malonyl-CoA molecules for elongation, respectively. In vitro assays and MS/MS analysis prove that both p-CA and p-HBA are firstly activated by the adenylation domain of FnsA. Phylogenetic analysis reveals that the PKS portion of FnsA shares high sequence homology with type I PKSs. Refactoring the biosynthetic pathway in yeast with the involvement of fnsA provides an alternative approach for the production of flavonoids such as isorhamnetin and acacetin.

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Section: Methodssupporting

confidence: 60%

A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin

Zhang

Zhou

et al. 2022

Nat Commun

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“…Curcumol ( 196 ), isolated from P . castaneae-mollissimae GQH87 derived from medicinal plant Artemisia annua , showed cytotoxicity against MCF-7, HepG2, and A549 with IC 50 values of 25.73, 65.18, and 178.32 μg/mL, respectively [ 87 ]. The cultivation of fungus Phomopsis sp.…”

Section: Bioactive Secondary Metabolites From Phomopsis mentioning

confidence: 99%

“…Oleanolic acid ( 209 ) was extracted from P . castaneae-mollissimae GQH87, which showed cytotoxicity against MCF-7, HepG2, and A549 with IC 50 values of 16.61, 39.53, and 40.08 μg/mL, respectively [ 87 ]. The structures of triterpenoids ( 207 – 209 ) are shown in Figure 8 .…”

Section: Bioactive Secondary Metabolites From Phomopsis mentioning

confidence: 99%

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Bioactive Secondary Metabolites of the Genus Diaporthe and Anamorph Phomopsis from Terrestrial and Marine Habitats and Endophytes: 2010–2019

Wang

et al. 2021

Microorganisms

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The genus Diaporthe and its anamorph Phomopsis are distributed worldwide in many ecosystems. They are regarded as potential sources for producing diverse bioactive metabolites. Most species are attributed to plant pathogens, non-pathogenic endophytes, or saprobes in terrestrial host plants. They colonize in the early parasitic tissue of plants, provide a variety of nutrients in the cycle of parasitism and saprophytism, and participate in the basic metabolic process of plants. In the past ten years, many studies have been focused on the discovery of new species and biological secondary metabolites from this genus. In this review, we summarize a total of 335 bioactive secondary metabolites isolated from 26 known species and various unidentified species of Diaporthe and Phomopsis during 2010–2019. Overall, there are 106 bioactive compounds derived from Diaporthe and 246 from Phomopsis, while 17 compounds are found in both of them. They are classified into polyketides, terpenoids, steroids, macrolides, ten-membered lactones, alkaloids, flavonoids, and fatty acids. Polyketides constitute the main chemical population, accounting for 64%. Meanwhile, their bioactivities mainly involve cytotoxic, antifungal, antibacterial, antiviral, antioxidant, anti-inflammatory, anti-algae, phytotoxic, and enzyme inhibitory activities. Diaporthe and Phomopsis exhibit their potent talents in the discovery of small molecules for drug candidates.

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Endophytic Fungi and Their Impact on Agroecosystems

Khan

2020

Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation

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Secondary Metabolites of an Endophytic Fungus Phomopsis castaneae-mollissimae

Cited by 6 publications

References 4 publications

A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin

A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin

Bioactive Secondary Metabolites of the Genus Diaporthe and Anamorph Phomopsis from Terrestrial and Marine Habitats and Endophytes: 2010–2019

Endophytic Fungi and Their Impact on Agroecosystems

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