Bioactive Secondary Metabolites of the Genus Diaporthe and Anamorph Phomopsis from Terrestrial and Marine Habitats and Endophytes: 2010–2019

Xu, Tang-Chang; Lu, Yihan; Wang, Junfei; Song, Zhiqiang; Hou, Yachen; Liu, Sisi; Liu, Chuansheng; Wu, Shao‐Hua

doi:10.3390/microorganisms9020217

Cited by 46 publications

(51 citation statements)

References 171 publications

(126 reference statements)

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“…The genus Diaporthe is a rich source of bioactive secondary metabolites, including volatile and non-volatile compounds, which have been shown to be effective against plant pathogens ( Singh et al, 2011 ; Noriler et al, 2019 ; Savi et al, 2020 ; Xu et al, 2021 ). This genus has also been recognized as a producer of several new compounds of great interest in biotechnology ( Nicoletti and Fiorentino, 2015 ; Santos et al, 2016 ; Tahir et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.

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Section: Discussionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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“…As examined in some of the earlier reviews [1,2] there are several ways of getting new scaffolds and classes of antimicrobial bioactive compounds. In the domain of natural products, one of the most demonstrated ways is studying less explored species and genera of microbes [3][4][5]. Investigating unexplored ecological units on the globe synergizes with the concept of investigating the least or not explored species of microbes.…”

Section: Introductionmentioning

confidence: 99%

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Deshmukh

Dufossé²,

Chhipa

et al. 2022

JoF

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Antibiotic resistance is becoming a burning issue due to the frequent use of antibiotics for curing common bacterial infections, indicating that we are running out of effective antibiotics. This has been more obvious during recent corona pandemics. Similarly, enhancement of antimicrobial resistance (AMR) is strengthening the pathogenicity and virulence of infectious microbes. Endophytes have shown expression of various new many bioactive compounds with significant biological activities. Specifically, in endophytic fungi, bioactive metabolites with unique skeletons have been identified which could be helpful in the prevention of increasing antimicrobial resistance. The major classes of metabolites reported include anthraquinone, sesquiterpenoid, chromone, xanthone, phenols, quinones, quinolone, piperazine, coumarins and cyclic peptides. In the present review, we reported 451 bioactive metabolites isolated from various groups of endophytic fungi from January 2015 to April 2021 along with their antibacterial profiling, chemical structures and mode of action. In addition, we also discussed various methods including epigenetic modifications, co-culture, and OSMAC to induce silent gene clusters for the production of noble bioactive compounds in endophytic fungi.

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“…Diaporthe spp. are distributed widely in many plants and have been regarded as potential sources for producing diverse bioactive metabolites ( Xu et al, 2021 ). Most of the compounds derived from this genus are polyketides, exhibiting various bioactivities, including cytotoxic ( Carvalho et al, 2018 ), antifungal ( Luo et al, 2018 ), antibacterial ( Niaz et al, 2021 ), antiviral ( Yang et al, 2020 ), antioxidant ( Da Rosa et al, 2020 ), and anti-inflammatory activities ( Fan et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1

Zhang

Liu

et al. 2022

Front. Microbiol.

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Endophytic fungi of medicinal plants are important sources of active natural products. In this study, 26 fungi were isolated from Artemisia argyi, which were belonging to eight genera, namely, Alternaria, Fusarium, Chaetomium, Phoma, Diaporthe, Trichoderma, Gibberella, and Colletotrichum. The antimicrobial activities of all fungal extracts were tested by using the cup-plate method against Staphylococcus aureus, Salmonella enteritidis, and Fusarium graminearum. The results demonstrated that 25 extracts (96%) exhibited inhibitory activity against at least one of the tested pathogenic microorganisms. The strain Diaporthe sp. AC1, which showed good antimicrobial activity and high yield of crude extract from fermentation, was selected for the study of secondary metabolites. The crude extract of strain AC1 was purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC, and finally, a new compound phomopsolide G (1), together with three known phomopsolides (2–4) and four other known compounds (5–8), was obtained. The structures of the compounds were elucidated by NMR and/or HR-MS spectroscopy. Microdilution method and MTT colorimetry were used to determine the bioactivity of the compounds. The study demonstrated that the new compound 1 had moderate antifungal activity against F. graminearum, Fusarium moniliforme, and Botrytis cinerea and weak antibacterial activity against Staphylococcus aureus. Compound 1 also showed weak cytotoxicity against HepG2, A549, and MDA-MB-231, with IC50 values of 89.91, 107.65, and 53.97 μM. Additionally, other compounds also exhibited antimicrobial and/or cytotoxic activities. The findings provided the basis for searching drug and agricultural lead compounds from A. argyi-associated fungi resources.

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Bioactive Secondary Metabolites of the Genus Diaporthe and Anamorph Phomopsis from Terrestrial and Marine Habitats and Endophytes: 2010–2019

Cited by 46 publications

References 171 publications

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1

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