Secondary Metabolites From Micromonospora Sp. (G044)

Tuan, Cao Duc; Hương, Đoàn Thị Mai; Ngan, Truong Bich; Quyên, Vũ Thị; Minh, Le Quoc; Murphy, Brian T.; Minh, Châu Văn; Cường, Phạm Văn

doi:10.15625/2525-2518/55/3/8641

Cited by 3 publications

(5 citation statements)

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“…Bacillus, Pseudovibrio, Ruegeria, Vibrio, and Streptomyces, which were not detected or were detected at very low relative abundance in the same sponge specimens by direct Illumina MiSeq amplicon sequencing analysis (Chapter 2). These results are in line with other efforts in cultivation of Vietnamese sponge-associated microbes for the discovery of novel natural compounds where especially the genera Bacillus and Streptomyces were frequently recovered (Tuan et al, 2017;Cao et al, 2019;Vu et al, 2019;Cao et al, 2020;Quang et al, 2020;Trinh et al, 2020;Quang et al, 2021a;Quang et al, 2021b;Kim et al, 2022;Trinh et al, 2022). At the OTU level, only six OTUs out of 921 OTUs detected by MiSeq sequencing of 16S rRNA gene amplicons were recovered by cultivation, but the abundance of the shared OTUs was distinct for the two methods (Chapter 3).…”

Section: Little Overlap Of Vietnamese Sponge Prokaryotes Captured By ...supporting

confidence: 86%

“…Only one isolated strain with strong antimicrobial activity was selected because of the time-consuming procedures, limiting the usefulness of this approach for the remaining bioactive strains from this study, but also in general. Indeed, the efforts to discover novel natural compounds from Vietnamese sponge-associated microbes in recent studies have resulted in the isolation of just a few compounds, of which the majority was already known (Tuan et al, 2017;Cao et al, 2019;Vu et al, 2019;Cao et al, 2020;Quang et al, 2020;Trinh et al, 2020;Quang et al, 2021a;Quang et al, 2021b;Kim et al, 2022;Trinh et al, 2022). Therefore, alternative approaches are urgently needed to discover new compounds, including the development of new cultivation methods enhancing the cultivation of novel microbes.…”

Section: Re-isolation Of Secondary Metabolites By Traditional Cultiva...mentioning

confidence: 99%

“…Compared to studies on sponge taxonomy and sponge-derived natural products, Vietnamese sponge-associated microbiota and their natural products have only been studied in the last few years and remain largely unexplored. Cultivation-dependent investigations focused on the isolation of sponge-associated microbes that produce bioactive compounds (Tuan et al, 2017;Cao et al, 2019;Vu et al, 2019;Cao et al, 2020;Quang et al, 2020;Trinh et al, 2020;Quang et al, 2021a;Quang et al, 2021b;Kim et al, 2022;Trinh et al, 2022), resulting in the isolation of nearly 80 compounds with a few new antimicrobial and cytotoxic compounds. Apart from cultivation studies, investigations of Vietnamese sponge microbiota by cultivation-independent methods have yielded several interesting insights into Vietnamese sponge microbiomes such as host-specificity, the influence of environmental quality on their microbiomes, as well as the role of seawater in bacteria recruitment and microbiome stability in sponges (Swierts et al, 2018;Turon et al, 2018;Turon et al, 2019a;Turon et al, 2019b;Cleary et al, 2020;Turon and Uriz, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of prokaryotic communities associated with sponges in Vietnam and their secondary metabolite biosynthetic potential

Ton That Huu Dat

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Sponge-associated prokaryotic diversity has been studied from a wide range of marine environments across the globe. However, for certain regions, e.g. Vietnam, Thailand, Cambodia and Singapore, an overview of the sponge-associated prokaryotic communities is still pending. In this study we characterized the prokaryotic communities from 27 specimens, comprising 18 marine sponge species, sampled from the central coastal region of Vietnam. Illumina MiSeq sequencing of 16S rRNA gene fragments was used to investigate spongeassociated bacterial and archaeal diversity. Overall, 14 bacterial phyla and one archaeal phylum were identified among all 27 samples. The phylum Proteobacteria was present in all sponges and the most prevalent phylum in 15 out of 18 sponge species, albeit with pronounced differences at the class level. In contrast, Chloroflexi was the most abundant phylum in Halichondria sp., whereas Spirastrella sp. and Dactylospongia sp. were dominated by Actinobacteria. Several bacterial phyla such as Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Deferribacteres, Gemmatimonadetes, and Nitrospirae were found in two-thirds of the sponge species. Moreover, the phylum Thaumarchaeota (Archaea), which is known to comprise nitrifying archaea, was highly abundant among the majority of the 18 investigated sponge species. Altogether, this study demonstrates that the diversity of prokaryotic communities associated with Vietnamese sponges is comparable to sponge-prokaryotic assemblages from well-documented regions. Furthermore, the phylogenetically divergent sponges hosted species-specific prokaryotic communities, thus demonstrating the influence of host identity on the composition and diversity of the associated communities. Therefore, this high-throughput 16S rRNA gene amplicon analysis of Vietnamese sponge-prokaryotic communities provides a foundation for future studies on sponge symbiont function and sponge-derived bioactive compounds from this region.

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Section: Little Overlap Of Vietnamese Sponge Prokaryotes Captured By ...supporting

confidence: 86%

Section: Re-isolation Of Secondary Metabolites By Traditional Cultiva...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of prokaryotic communities associated with sponges in Vietnam and their secondary metabolite biosynthetic potential

Ton That Huu Dat

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“…In the 1 H-NMR spectrum of 5, the proton signals were close to those of 4, except for the presence of one methylene group in the aliphatic region of 4 replaced by an oxymethine group in 5. Comparison of NMR data with the reference data and R f value of commercially available uridine in the laboratory through using co-TLC method revealed the structure of 5 as uridine [15].…”

Section: Resultsmentioning

confidence: 92%

Chemical Constituents of Marine-Derived Actinomycete Streptomyces fradiae G650

Doan¹,

Pham²,

Nguyen³

et al. 2022

Vietnam J. Sci. Technol.

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In our continuing search for bioactive molecules, we collected a Streptomyces fradiae strain cultured from a prickly pen shell sample obtained at Van Phong, Khanh Hoa province, Vietnam. The ethyl acetate extract of Streptomyces fradiae G650 exhibited the strong inhibition against a panel of gram-positive bacteria including Enterococcus faecalis ATCC 29212, Staphylococcus aurerus ATCC 25923, Bacillus cereus ATCC 14579, and the yeast Candida albicans with the MIC values of 8, 32, 32, 8 µg/mL, respectively. As a result, from the marine-derived actinomycete Streptomyces fradiae G650, six compounds were isolated, including15α-hydroxyneoline (1), cyclo-(Leu-Tyr) (2), cyclo-(Pro-Gly) (3), 2'-deoxyuridine (4), uridine (5), and adenine (6). These compounds were structurally characterized via 1D and 2D NMR spectroscopic, mass spectrometric analyses and by comparison of the spectral data with those of previously reported data. To the best of our knowledge, 15α-hydroxyneoline (1) was reported to be isolated from microorganism for the first time.

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“…It has also been isolated from a Micromonospora spp . strain associated with the marine sponge Tethya aurantium ( Tuan et al, 2017 ) as well as from Microbispora aerata from Antarctica ( Bratchkova and Ivanova, 2011 ). In Bacillus spp .…”

Section: Discussionmentioning

confidence: 99%