Secondary Metabolites from Astragalus lycius and Their Cytotoxic Activities

Abstract

O-β-D-glucopyranoside (8) by spectroscopic methods including 1 Hand 13 C-NMR and HR-MS experiments, and by comparison with literature values. Compounds 1-7 are reported for the first time from Astragalus taxa. All of the compounds were tested for their cytotoxic activities against a number of cancer cell lines. Among them, only 6 exhibited significant activity against human colon carcinoma (HT-29) at 2.69 M concentration.

Biological Effects of Saponin Fractions from Astragalus verrucosus in Tumor and Non-tumor Human cells

“…Annexin-V/PI double staining demonstrated that SF(3) tumor growth inhibitory effect on HCT116 cells was associated to the induction of apoptotic cell death. In fact, as observed in Figure 3A, 15.84 µg/mL (twice the GI 50 ) of SF (3) induced early and late apoptosis. Western blot analysis showed that the SF (3)-induced apoptosis in HCT116 cells was associated with an increase in p53 protein, up-regulation of Bax (a p53 downstream pro-apoptotic target of the Bcl-2 family), as well as occurrence of PARP cleavage (a result of caspase-3 activation) (Figure 3B).…”

“…Other than cell membrane permeabilization, these compounds have shown cholesterol-binding, antiviral, immunomodulatory, antimicrobial, cytostatic, and antitumor activity [1,[11][12][13]. Different studies reported the ability of saponins, in particular triterpenoid saponins, to inhibit the proliferation of several tumor cell lines through induction of cell cycle arrest and/or apoptosis [1,5,9,[11][12][13][14][15]. Moreover, it has been shown that saponins can also have nongenotoxic and non-cytotoxic effects in human lymphocytes [16,17].…”

Evaluation of cytotoxic and apoptotic effects of the extracts and phenolic compounds of Astragalus globosus Vahl and Astragalus breviflorus DC

“…Astragalus species have saponins, flavonoids, phenylpropanoids, alkaloids, steroids, and polysaccharides and show anti-inflammatory, immunoregulatory, anti-tumour, antioxidative, antidiabetic, antiviral, and hepatoprotective activities ( Li et al, 2014 , Yang et al, 2013 ). In the literature, many studies have shown that Astragalus species and their active compounds exhibit cytotoxic effects on colon carcinoma (HCT-116, HT-29), hepatocellular carcinoma (HEPG2), myeloid leukaemia (K 562), lymphocytic leukaemia (SKW-3), osteosarcoma (MG63), and breast cancer (MCF-7, MDA-MB-231) cell lines ( Ibrahim et al, 2013 , Horo et al, 2016 , Salem et al, 2020 , Ionkova et al, 2010 ). Astragalus globosus Vahl (syn.…”

New phenol and chromone derivatives from the endolichenic fungusDaldiniaspecies and their antiviral activities

“…The known metabolites were identied as 2-phenylethyl-b-Dglucopyranoside (4), 21 stachyline C (5), 22 3-methoxy-4-hydroxyphenylethanol (6), 23 3-hydroxy-4-methoxy-phenylethanol (7), 24 p-hydroxyphenethyl alcohol (8), 25 (5R,7S)-5,7-dihydroxy-2-methyl-5,6,7,8-tetrahydro-4H-chromen-4-one (11), 20 (5R,7S)-5,7-dihydroxy-2-propyl-5,6,7,8-tetrahydro-4H-chromen-4-one (12) 20 by comparison of their NMR and MS data with those reported. Compounds 1-12 were evaluated for anti-inuenza A virus (IAV), anti-Zika virus (ZIKV), and antibacterial activities.…”