Secondary ion mass spectrometry

Goldsmith, Jack G.; Linton, Richard W.

doi:10.1016/b978-012371020-8/50017-2

Cited by 2 publications

(1 citation statement)

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“…At the cellular scale, secondary ion mass spectrometry (SIMS) provides a means of detecting and tracking these labeled molecules that are, in a biological sense, indistinguishable from unlabeled molecules. Cellular-scale analysis is a relatively recent development of the SIMS technique, but significant advancements in both static (surface) and dynamic (bulk) SIMS technologies now permit a wide range of applications to biological and biomedical research (see Chandra et al, 2000;Clerc et al, 1997;Goldsmith et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Subcellular imaging of isotopically labeled carbon compounds in a biological sample by ion microprobe (NanoSIMS)

Clode

Stern

Marshall

2007

Microscopy Res & Technique

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Here we demonstrate the technique of nanoscale secondary ion mass spectrometry, utilizing the Cameca NanoSIMS50 ion microprobe, to detect and image the metabolism of an isotopically labeled compound (NaH(13)CO(3)) in a biological sample. In particular, we have designed and verified protocols for imaging the subcellular distribution and determining the relative abundance of labeled (13)C, within the coral Galaxea fascicularis. Analyses were conducted on 1-mum thick sections of resin-embedded material, using both scanned (mapping) and static (spot analysis) Cs(+) primary ion beam of approximately 100 nm diameter. Using these samples we establish that NanoSIMS has adequate mass resolution to reliably distinguish (13)C from potential isobaric interference by (12)C(1)H and that data extracted from ion maps are comparable to those acquired by spot analyses. Independent of the method of acquisition, ratioing of (13)C to the naturally abundant (12)C is essential if meaningful data, which can be statistically compared to standard and control samples, are to be obtained. These results highlight the potential of NanoSIMS for intracellular tracking of a variety of organic and inorganic compounds labeled with stable isotopes of C, N, O, S, P, and halogens.

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Section: Introductionmentioning

confidence: 99%