The key ingredient of this paper is the universal central extension of the generalized orthosymplectic Lie superalgebra osp m|2n (R, − ) coordinatized by a unital associative superalgebra (R, − ) with superinvolution. Such a universal central extension will be constructed via a Steinberg orthosymplectic Lie superalgebra coordinated by (R, − ). The research on the universal central extension of osp m|2n (R, − ) will yield an identification between the second homology group of the generalized orthosymplectic Lie superalgebra osp m|2n (R, − ) and the first Z/2Z-graded skew-dihedral homology group of (R, − ) for (m, n) = (2, 1), (1, 1). The universal central extensions of osp 2|2 (R, − ) and osp 1|2 (R, − ) will also be treated separately.MSC(2010): 17B05, 19D55.