Second harmonic imaging of plants tissues and cell implosion using two‐photon process in ZnO nanoparticles

Urban, Ben E.; Neogi, Purnima B.; Butler, Sween; Fujita, Yasuhisa; Neogi, Arup

doi:10.1002/jbio.201100076

Cited by 17 publications

(12 citation statements)

References 29 publications

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“…No significant phototoxicity was obtained with the excitation of a 1064 nm picosecond laser. 187 However, the heating effect caused cell implosion in plant tissues when the laser light wavelength shifted to 710 nm, which was followed by TPA. This wavelength selection is necessary to avoid damage to biological tissues.…”

Section: Lanthanide-doped Upconverting Nps (Ucnps)mentioning

confidence: 99%

Revisiting the classification of NIR-absorbing/emitting nanomaterials for in vivo bioapplications

et al. 2016

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With the development of nonlinear optics and new imaging methods, near-infrared (NIR) light can excite contrast agents to probe biological specimens both functionally and structurally with a deeper imaging depth and a higher spatial resolution than linear optical approaches. There is considerable and growing interest in how biological specimens respond to NIR light. Moreover, the visible absorption band of most functional nanomaterials becomes NIR-excitable through multiphoton processes, thus allowing multifunctional imaging and combined therapy with noble metal and magnetic nanoparticles both in vitro and in vivo. A groundbreaking example is the use of different laser techniques to excite single-type NIR-absorbing/emitting nanomaterials to produce multiphoton emission by femtosecond lasers using either a remote control system for photodynamic therapy or photo-induced chemical bond dissociation. These techniques provided superior anatomical resolution and detection sensitivity for in vivo tumor-targeted imaging than those offered by conventional methods. Here we summarize the most recent progress in the development of smart NIR-absorbing/emitting nanomaterials for in vivo bioapplications.

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Section: Lanthanide-doped Upconverting Nps (Ucnps)mentioning

confidence: 99%

Revisiting the classification of NIR-absorbing/emitting nanomaterials for in vivo bioapplications

et al. 2016

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“…As shown in Figure 3, the SHG emission spectra from the ZnO NPs can be observed with tunable wavelengths of the incident laser [89], and the SHG efficiency is unaffected by the dispersion of ZnO NPs in water [80]. The SHG signal from ZnO NPs is spectrally well-defined and is dependent on the full-width at half maxima of the incident laser pulse.…”

Section: Biological Imaging By Zno Npsmentioning

confidence: 99%

Section: Biological Imaging By Zno Npsmentioning

confidence: 99%

Photoluminescent ZnO Nanoparticles and Their Biological Applications

2015

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During the past decades, numerous achievements concerning luminescent zinc oxide nanoparticles (ZnO NPs) have been reported due to their improved luminescence and good biocompatibility. The photoluminescence of ZnO NPs usually contains two parts, the exciton-related ultraviolet (UV) emission and the defect-related visible emission. With respect to the visible emission, many routes have been developed to synthesize and functionalize ZnO NPs for the applications in detecting metal ions and biomolecules, biological fluorescence imaging, nonlinear multiphoton imaging, and fluorescence lifetime imaging. As the biological applications of ZnO NPs develop rapidly, the toxicity of ZnO NPs has attracted more and more attention because ZnO can produce the reactive oxygen species (ROS) and release Zn2+ ions. Just as a coin has two sides, both the drug delivery and the antibacterial effects of ZnO NPs become attractive at the same time. Hence, in this review, we will focus on the progress in the synthetic methods, luminescent properties, and biological applications of ZnO NPs.

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“…Among these properties, the optical properties of ZnO nanowires or NRs have been the focus of many studies because this kind of nanoobjects can be easily synthesized and has potential applications in making optoelectronic devices. In recent years, the nonlinear optical properties of ZnO nanoobjects have attracted much attention owing to their possible applications in the field of biophotonics [23][24][25]. Under the excitation of a femtosecond (fs) laser in the near infrared region, upconverted signals such as second harmonic generation (SHG) [26] and two-photon-induced luminescence (TPL) [27][28][29] can be generated in ZnO nanoobjects, making them attractive and promising candidates for bioimaging.…”

Section: Introductionmentioning

confidence: 99%

Competition between second harmonic generation and two-photon-induced luminescence in single, double and multiple ZnO nanorods

Dai¹,

Zeng²,

Lan³

et al. 2013

Opt. Express

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The nonlinear optical properties of single, double and multiple ZnO nanorods (NRs) were investigated by using a focused femtosecond (fs) laser beam. The excitation wavelength of the fs laser was intentionally chosen to be 754 nm at which the energy of two photons is slightly larger than that of the exciton ground state but smaller than the bandgap energy of ZnO. Second harmonic generation (SHG) or/and two-photon-induced luminescence (TPL) were observed and their dependences on excitation density were examined. For single ZnO NRs, only SHG was observed even at the highest excitation density we used in the experiments. The situation was changed when the joint point of two ZnO NRs perpendicular to each other was excited. In this case, TPL could be detected at low excitation densities and it increased rapidly with increasing excitation density. At the highest excitation density of ~15 MW/cm(2), the intensity of the TPL became comparable to that of the SHG. For an ensemble of ZnO NRs packed closely, a rapid increase of TPL with a slope of more than 7.0 and a gradual saturation of SHG with a slope of ~0.34 were found at high excitation densities. Consequently, the nonlinear response spectrum was eventually dominated by the TPL at high excitation densities and the SHG appeared to be very weak. We interpret this phenomenon by considering both the difference in electric field distribution and the effect of heat accumulation. It is suggested that the electric field enhancement in double and multiple NRs plays a crucial role in determining the nonlinear response of the NRs. In addition, the reduction in the bandgap energy induced by the heat accumulation effect also leads to the significant change in nonlinear response. This explanation is supported by the calculation of the electric field distribution using the discrete dipole approximation method and the simulation of temperature rise in different ZnO NRs based on the finite element method.

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Second harmonic imaging of plants tissues and cell implosion using two‐photon process in ZnO nanoparticles

Cited by 17 publications

References 29 publications

Revisiting the classification of NIR-absorbing/emitting nanomaterials for in vivo bioapplications

Revisiting the classification of NIR-absorbing/emitting nanomaterials for in vivo bioapplications

Photoluminescent ZnO Nanoparticles and Their Biological Applications

Competition between second harmonic generation and two-photon-induced luminescence in single, double and multiple ZnO nanorods

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