Seçilen Bitkilerin DMSO Özütlerinin Antibiyotik Dirençli Klinik İzolatlara Karşı Antibakteriyel Aktivitesi

Abstract

In this study, we aimed to find out new herbal materials that are able to inhibit the growth of the P. aeruginosa and E.coli clinical isolates that has antibiotic resistance. Clinical isolates used in this research are E. coli (n=1) and P.aeruginosa (n=1). Antibiotic susceptibility profiles of E. coli and P. aeruginosa were determined using e-test. Plants were collected in Trabzon region of Turkey are Calendula officinalis, Hypericum perforatum and Glycyrrhiza glabra. DMSO were used as solvent and solid-liquid extraction was employed. Micro-dilution method was preferred fo the determination of the minimum inhibitory concentration (MIC). MIC results were obtained through observation of turbidities. According to E-test results, while P. aeruginosa was resistant to piperacillin, piperacillin/tazobactam, meropenem and ceftazidime, E. coli was resistant to piperacillin, cefotaxime and ceftazidime. DMSO extract of Calendula officinalis showed very strong activity against PA1 with the best MIC (5 mg/mL). DMSO extract of three plant had lower MIC values (5-10 mg/ml) for EC1 and PA1 than ampicillin. In future studies antibacterial activity of different solvents extracts of these plants and other plants against antibiotic resistant clinical isolates will be examined. Natural products from plants are promising in fighting with antibiotic-resistant bacteria.

Chemical Analysis and Antibacterial Activity of Glycyrrhiza glabra roots

“…However, the ethanolic extract of G. glabra contained tannins and terpenoids, table 1. [22,23,24] in which G. glabra extract showed positive inhibitory antibacterial activity against Gram-positive and Gram-negative species such as S. aureus, P. aeruginosa and E. coli Chabuck et al [25]According to our study, G. Glabra exhibited activity against tested bacterial isolates with inhibition zone ranging 18-25mm; highest inhibition was seen against E. coli (25mm) and P. aerogenosa (24 mm). In contrast, Sultana et al [26] recorded antimicrobial efficacy of the G. Glabra extract against S. aureus and E.coli , but not P. aeruginosa, while, Soulef et al [27] show that G. glabra L extract had an antibacterial effect against S. aureus and P. aeruginosa, but not the E. coli.…”